LOCUS       sequence-374167-       16481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1
            in a bacterial and insect expression system. Internal_Reference: 
            SMC1326.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16481)
  TITLE     ASAP - PI3KC3-C1 related projects
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGBdest-GST-PI3Kc1
FEATURES             Location/Qualifiers
     source          1..16481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(27..560)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(749..754,755..771,772..777))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        965..1056
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1112..1765
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1723..1745
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1772..1795
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3325..3342
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3486..3620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3792..3883
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     RBS             5324..5332
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6653..6670
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6814..6948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7120..7211
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             11394..11411
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    11555..11689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        11861..11952
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             13411..13428
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    13572..13706
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  13866..14031
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     primer_bind     complement(14302..14321)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(14387..15202)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(14387..15202)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     15110..15129
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     15194..15213
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      15324..15912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15813..15832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16066..16083
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  16217..16441
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 aagccgatct cggcttgaac gaattgttag gtggcggtac ttgggtcgat atcaaagtgc
       61 atcacttctt cccgtatgcc caactttgta tagagagcca ctgcgggatc gtcaccgtaa
      121 tctgcttgca cgtagatcac ataagcacca agcgcgttgg cctcatgctt gaggagattg
      181 atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag atcatagata
      241 tagatctcac tacgcggctg ctcaaacttg ggcagaacgt aagccgcgag agcgccaaca
      301 accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct tactacggag caagttcccg
      361 aggtaatcgg agtccggctg atgttgggag taggtggcta cgtctccgaa ctcacgaccg
      421 aaaagatcaa gagcagcccg catggatttg acttggtcag ggccgagcct acatgtgcga
      481 atgatgccca tacttgagcc acctaacttt gttttagggc gactgccctg ctgcgtaaca
      541 tcgttgctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
      601 acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc
      661 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
      721 gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt atgtcaactg
      781 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
      841 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcacctg tattcagtcg
      901 actggtacca acactgcgtt taaacactag tatcgattcg cgacctactc cggaatatta
      961 atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt
     1021 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc
     1081 accatcgggc gacggatccc ggtccgaaac catgtcccct atactaggtt attggaaaat
     1141 taagggcctt gtgcaaccca ctcgacttct tttggaatat cttgaagaaa aatatgaaga
     1201 gcatttgtat gagcgcgatg aaggtgataa atggcgaaac aaaaagtttg aattgggttt
     1261 ggagtttccc aatcttcctt attatattga tggtgatgtt aaattaacac agtctatggc
     1321 catcatacgt tatatagctg acaagcacaa catgttgggt ggttgtccaa aagagcgtgc
     1381 agagatttca atgcttgaag gagcggtttt ggatattaga tacggtgttt cgagaattgc
     1441 atatagtaaa gactttgaaa ctctcaaagt tgattttctt agcaagctac ctgaaatgct
     1501 gaaaatgttc gaagatcgtt tatgtcataa aacatattta aatggtgatc atgtaaccca
     1561 tcctgacttc atgttgtatg acgctcttga tgttgtttta tacatggacc caatgtgcct
     1621 ggatgcgttc ccaaaattag tttgttttaa aaaacgtatt gaagctatcc cacaaattga
     1681 taagtacttg aaatccagca agtatatagc atggcctttg cagggctggc aagccacgtt
     1741 tggtggtggc gaccatcctc caaaatcgga tctggaagtt ctgttccagg ggcccatggc
     1801 atcgccaagc ggaaaaggag caagagccct ggaagcaccc ggctgcggac caagaccact
     1861 cgcccgtgac ctcgttgata gcgtggacga cgctgagggt ctgtacgtgg ccgtcgaacg
     1921 ttgccctctg tgcaacacca ctcgccgtag gctgacctgc gctaagtgcg tgcagagcgg
     1981 tgacttcgtc tacttcgacg gacgcgaccg cgagcgtttc atcgacaaga aggaaaggct
     2041 gtccagactg aagagcaagc aggaggagtt ccagaaggag gtcctgaagg ctatggaggg
     2101 caagtggatc actgaccagc tgcgctggaa gatcatgtct tgcaagatgc gtatcgagca
     2161 gctgaagcag accatctgca agggcaacga ggaaatggag aagaactctg aaggactgct
     2221 gaagactaag gaaaagaacc agaagctgta ctcacgcgct cagcgtcacc aggagaagaa
     2281 ggaaaagatc cagaggcaca acagaaagct gggtgacctg gtggagaaga agaccatcga
     2341 cctgcgcagc cactacgaac gtctggccaa cctgagacgc tcccacatcc tggagctgac
     2401 cagcgtcatc ttccccatcg aggaagtgaa gactggcgtc agggacccag ctgacgtgtc
     2461 cagcgagagc gactctgcca tgacctcttc aactgtctcc aagctggctg aagcccgtag
     2521 gaccacttac ctgagcggac gctgggtgtg cgacgaccac aacggagaca cctcaatctc
     2581 catcactggt ccctggatct ccctgccaaa caacggagac tactctgctt actactcatg
     2641 ggtcgaggaa aagaagacca ctcagggtcc tgacatggag cagtctaacc ccgcttacac
     2701 catctcagct gccctgtgct acgccactca gctggtgaac atcctgtctc acatcctgga
     2761 cgtcaacctg ccaaagaagc tgtgcaactc cgagttctgc ggcgaaaacc tgagcaagca
     2821 gaagttcacc cgcgctgtga agaagctgaa cgccaacatc ctgtacctgt gcttctcaca
     2881 gcacgtcaac ctggaccagc tgcaacctct gcacactctg cgtaacctga tgtacctggt
     2941 gtctccatcc agcgagcacc tgggaaggtc aggtccattc gaagtcagag ctgacctgga
     3001 ggaaagcatg gagttcgtgg accctggcgt cgccggagag tctgacgaat caggtgacga
     3061 gagggtgtcc gacgaggaaa ccgacctggg cactgactgg gaaaacctgc caagccctag
     3121 attctgcgac atccccagcc agtctgtgga agtctcacaa tctcagagca cccaggcctc
     3181 ccctcccatc gcttcttcat ccgccggtgg catgatctcg tcagccgcag catccgtcac
     3241 ttcgtggttc aaggcataca ctggacatag gtaacgccat taacctgatg ttctggggaa
     3301 tataagcttg cggccgcact cgagcaccac caccaccacc actgacatgc ggtaccaagc
     3361 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
     3421 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     3481 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3541 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3601 tatcttatca tgtctggatc tgatcactgc ttgagcctag aagatccggc tgctaacaaa
     3661 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactatc ataaccccta
     3721 gggtataccc atctaattgg actagtttaa acactagtat cgattcgcga cctactccgg
     3781 aatattaata gatcatggag ataattaaaa tgataaccat ctcgcaaata aataagtatt
     3841 ttactgtttt cgtaacagtt ttgtaataaa aaaacctata aatattccgg attattcata
     3901 ccgtcccacc atcgggcgac ggatcccggt ccgaaaccat gggcgaagcg gagaaattcc
     3961 actacatcta ctcttgcgac ttggacatca acgttcaact caaaatcggt tcactcgaag
     4021 gtaaacgcga gcagaagtcc tacaaggccg tgctggaaga ccctatgctg aagttctcag
     4081 gtctgtacca ggaaacctgc tccgacctgt acgtgacttg ccaggtcttc gctgagggca
     4141 agcctctggc tctgcccgtg cgcaccagct acaaggcttt ctctactcgt tggaactgga
     4201 acgagtggct gaagctgccc gtcaagtacc ccgacctgcc acgtaacgct caggtggccc
     4261 tgaccatctg ggacgtgtac ggtcccggca aggccgtgcc agtcggtggc accactgtgt
     4321 cactgttcgg caagtacgga atgttccgcc agggaatgca cgacctgaaa gtgtggccaa
     4381 acgtcgaggc tgacggctcc gaaccaacca agactcctgg acgtacctcc agcactctgt
     4441 cagaggacca gatgtccagg ctggctaagc tgaccaaggc ccacagacag ggtcacatgg
     4501 tgaaggtgga ctggctggac cgcctgactt tccgtgagat cgaaatgatc aacgagagcg
     4561 aaaagcgctc ttcaaacttc atgtacctga tggtggagtt ccgttgcgtc aagtgcgacg
     4621 acaaggaata cggtatcgtg tactacgaga aggacggcga cgaatccagc cctatcctga
     4681 cctctttcga gctggtgaag gtcccagacc ctcagatgag catggagaac ctggtcgaat
     4741 ctaagcacca caagctggct cgctccctgc gtagcggtcc atctgaccac gacctgaagc
     4801 caaacgctgc cactagggac cagctgaaca tcatcgtgag ctaccctccc accaagcagc
     4861 tgacttacga ggaacaggac ctggtctgga agttcagata ctacctgacc aaccaggaga
     4921 aggctctgac taagttcctg aagtgcgtga actgggacct gccacaggag gctaagcagg
     4981 ccctggaact gctgggcaag tggaagccaa tggacgtcga ggactctctg gaactgctgt
     5041 cttcacacta caccaaccca actgtgcgcc gttacgctgt cgccaggctg agacaggccg
     5101 acgacgaaga cctgctgatg tacctgctgc aactggtgca ggctctgaag tacgagaact
     5161 tcgacgacat caagaacgga ctggaaccta ccaagaagga cagccagtcc agcgtgtctg
     5221 agaacgtctc taactcaggt atcaactcag ccgaaatcga ctcttcacag atcatcacct
     5281 cacccctgcc atccgtgtcc agcccacctc ccgcttccaa gactaaggag gtccccgacg
     5341 gcgagaacct ggaacaggac ctgtgcacct tcctgatcag ccgcgcttgc aagaactcta
     5401 ctctggccaa ctacctgtac tggtacgtga tcgtcgagtg cgaagaccag gacacccagc
     5461 agcgtgaccc aaagactcac gaaatgtacc tgaacgtgat gaggagattc tcccaggctc
     5521 tgctgaaggg agacaagtca gtgagggtca tgagatccct gctggctgcc cagcagacct
     5581 tcgtggaccg cctggtccac ctgatgaagg ccgtccagag ggagagcgga aaccgcaaga
     5641 agaagaacga acgtctgcaa gctctgctgg gtgacaacga gaagatgaac ctgtctgacg
     5701 tggagctgat ccctctgccc ctggaacctc aggtcaagat caggggtatc atccctgaaa
     5761 ccgctactct gttcaagtca gctctgatgc cagcccagct gttcttcaag actgaagacg
     5821 gtggcaagta ccctgtgatc ttcaagcacg gcgacgacct gaggcaggac cagctgatcc
     5881 tgcaaatcat ctccctgatg gacaagctgc tgagaaagga gaacctggac ctgaagctga
     5941 ccccttacaa ggtgctggcc accagcacta agcacggctt catgcagttc atccagtctg
     6001 tgcccgtcgc tgaggtcctg gacactgaag gaagcatcca gaacttcttc cgtaagtacg
     6061 ctcctagcga gaacggtccc aacggcatct ctgccgaagt gatggacacc tacgtcaagt
     6121 cttgcgctgg ctactgcgtg atcacttaca tcctgggagt cggtgacagg cacctggaca
     6181 acctgctgct gaccaagact ggaaagctgt tccacatcga cttcggatac atcctgggtc
     6241 gcgaccctaa gcctctgcca cctcccatga agctgaacaa ggagatggtg gagggtatgg
     6301 gcggaaccca gtcagagcag taccaggagt tcaggaagca gtgctacact gccttcctgc
     6361 acctgcgccg ttactcaaac ctgatcctga acctgttctc cctgatggtg gacgctaaca
     6421 tcccagacat cgccctggag cctgacaaga ccgtgaagaa ggtgcaagac aagttcagac
     6481 tggacctgtc cgacgaggaa gctgtgcact acatgcagtc actgatcgac gaatccgtcc
     6541 acgcactctt cgcagcggtc gttgagcaga ttcataagtt cgcacaatac tggagaaaat
     6601 aacgccatta acctgatgtt ctggggaata taagcttgcg gccgcactcg agcaccacca
     6661 ccaccaccac tgacatgcgg taccaagctt gtcgagaagt actagaggat cataatcagc
     6721 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     6781 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     6841 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6901 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt
     6961 gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     7021 cgctgagcaa taactatcat aacccctagg gtatacccat ctaattggcc tggtttaaac
     7081 actagtatcg attcgcgacc tactccggaa tattaataga tcatggagat aattaaaatg
     7141 ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa
     7201 aacctataaa tattccggat tattcatacc gtcccaccat cgggcgacgg atcccggtcc
     7261 gaaaccatgg gtaaccagtt ggcaggcatc gccccctcgc agattttgag cgttgagtcc
     7321 tacttctccg acatccacga tttcgaatac gacaagtctc tgggctcaac cagattcttc
     7381 aaggtggcta gagccaagca ccgcgaagga ctggtggtcg tgaaggtctt cgccatccag
     7441 gaccctaccc tgcccctgac ttcttacaag caggagctgg aggaactgaa gatccgcctg
     7501 aactcagctc agaactgcct gccattccag aaggcctccg agaaggctag cgaaaaggct
     7561 gccatgctgt tccgtcagta cgtgagggac aacctgtacg acagaatctc tacccgccct
     7621 ttcctgaaca acatcgaaaa gagatggatc gctttccaga tcctgactgc cgtggaccag
     7681 gctcacaagt caggagtccg ccacggtgac atcaagaccg agaacgtcat ggtgacttct
     7741 tggaactggg tgctgctgac cgacttcgcc tcattcaagc caacttacct gcctgaagac
     7801 aaccccgctg acttcaacta cttcttcgac acctcccgcc gtaggacttg ctacatcgcc
     7861 cccgagagat tcgtggacgg tggcatgttc gctaccgagc tggaatacat gcgcgaccca
     7921 tccactcctc tggtggacct gaactccaac cagcgtacca ggggtgaact gaagagggct
     7981 atggacatct tctccgctgg ttgcgtgatc gctgagctgt tcactgaagg cgtccccctg
     8041 ttcgacctgt cacagctgct ggcttaccgt aacggccact tcttcccaga gcaggtgctg
     8101 aacaagatcg aggaccactc catcagggaa ctggtcaccc agatgatcca ccgtgaacca
     8161 gacaagaggc tggaggccga agactacctg aagcagcagc gcggcaacgc tttcccagaa
     8221 atcttctaca ccttcctgca accttacatg gctcagttcg ccaaggagac tttcctgagc
     8281 gccgacgaac gcatcctggt catccgcaag gacctgggta acatcatcca caacctgtgc
     8341 ggccacgacc tgcctgaaaa ggctgagggc gaacccaagg agaacggact ggtcatcctg
     8401 gtgtccgtga tcacctcatg cctgcaaact ctgaagtact gcgactccaa gctggctgcc
     8461 ctggaactga tcctgcacct ggctcctaga ctgagcgtgg agatcctgct ggaccgcatc
     8521 accccctacc tgctgcactt ctccaacgac agcgtcccaa gagtgcgcgc tgaggccctg
     8581 cgtaccctga ctaaggtcct ggccctggtc aaggaagtgc ccaggaacga catcaacatc
     8641 taccctgagt acatcctgcc cggaatcgct cacctggccc aggacgacgc taccatcgtg
     8701 cgtctggctt acgccgagaa catcgctctg ctggccgaaa ctgctctgag gttcctggag
     8761 ctggtgcagc tgaagaacct gaacatggaa aacgacccta acaacgagga aatcgacgag
     8821 gtcacccacc ccaacggtaa ctacgacact gagctgcaag ctctgcacga aatggtgcag
     8881 cagaaggtcg tgaccctgct gtctgaccca gaaaacatcg tcaagcagac cctgatggag
     8941 aacggaatca ctcgtctgtg cgtgttcttc ggtaggcaga aggccaacga cgtcctgctg
     9001 tcccacatga tcactttcct gaacgacaag aacgactggc acctgcgtgg cgctttcttc
     9061 gacagcatcg tcggtgtggc tgcctacgtg ggctggcagt ccagctctat cctgaagccc
     9121 ctgctgcaac agggtctgtc cgacgccgag gagttcgtca tcgtgaaggc cctgtacgct
     9181 ctgacctgca tgtgccagct gggtctgctg caaaagccac acgtgtacga gttcgcctcc
     9241 gacatcgctc cattcctgtg ccaccctaac ctgtggatca gatacggcgc tgtgggattc
     9301 atcaccgtcg tggcccgcca gatcagcact gctgacgtct actgcaagct gatgccatac
     9361 ctggaccctt acatcaccca gcctatcatc cagatcgaaa gaaagctggt gctgctgtcc
     9421 gtcctgaagg agcccgtgtc tcgttcaatc ttcgactacg ctctgaggtc taaggacatc
     9481 acttcactgt tccgccacct gcacatgcgt cagaagaaga ggaacggatc tctgcctgac
     9541 tgccctcccc cagaggaccc agctatcgct cagctgctga agaagctgct gtcacagggt
     9601 atgaccgagg aagaggaaga caagctgctg gccctgaagg acttcatgat gaagtccaac
     9661 aaggctaagg ccaacatcgt ggaccagagc cacctgcacg actcatccca gaagggtgtc
     9721 atcgacctgg ctgccctggg tatcaccggc agacaggtgg acctggtgaa gactaagcag
     9781 gagcctgacg acaagcgtgc taggaagcac gtgaagcagg actccaacgt caacgaggaa
     9841 tggaagtcca tgttcggcag cctggaccct cccaacatgc ctcaggccct gccaaagggc
     9901 agcgaccagg aagtgatcca gaccggaaag ccacctcgtt ctgagtcctc cgctggtatc
     9961 tgcgtccctc tgtcaacttc atcccaggtc cccgaggtga ccactgtcca gaacaagaag
    10021 cccgtgatcc cagtcctgtc ctccaccatc ctgccaagca cttaccagat ccgtatcacc
    10081 acttgcaaga ccgagctgca acagctgatc cagcagaaga gagagcagtg caacgccgaa
    10141 cgcatcgcta agcagatgat ggagaacgcc gagtgggaat ccaagccccc acctcccgga
    10201 tggaggccta agggtctgct ggtggcccac ctgcacgaac acaagagcgc tgtgaacaga
    10261 atccgcgtct ctgacgagca ctcactgttc gctacctgct ctaacgacgg cactgtcaag
    10321 atttggaact cccagaagat ggagggaaag accactacca ctcgctccat cctgacctac
    10381 agcagaatcg gaggtcgcgt gaagaccctg actttctgcc agggctccca ctacctggct
    10441 atcgccagcg acaacggagc cgtccagctg ctgggtatcg aggcttccaa gctgcctaag
    10501 tcacccaaga tccaccctct gcaatctcgt atcctggacc agaaggagga cggatgcgtc
    10561 gtggacatgc accacttcaa ctccggtgct cagagcgtgc tggcttacgc cactgtgaac
    10621 ggctcactgg tcggatggga cctgaggtca tccagcaacg cctggaccct gaagcacgac
    10681 ctgaagtccg gactgatcac tagcttcgct gtggacatcc accagtgctg gctgtgcatc
    10741 ggcacctcct ccggcactat ggcctgctgg gacatgcgtt tccagctgcc aatctccagc
    10801 cactgccacc cttcccgtgc taggatcaga cgcctgtcta tgcaccccct gtaccagtca
    10861 tgggtcatcg ctgccgtcca gggtaacaac gaagtgtcta tgtgggacat ggaaaccggc
    10921 gaccgtaggt tcactctgtg ggcctcttca gctccacctc tgtcagagct gcaaccctct
    10981 ccacactcag tccacggaat ctactgctcc cccgccgacg gtaacccaat cctgctgacc
    11041 gctggcagcg acatgaagat ccgtttctgg gacctggcct acccagagag gtcttacgtc
    11101 gtggctggtt caacctccag cccttccgtg agctactacc gcaagatcat cgagggaact
    11161 gaagtcgtgc aggaaatcca gaacaagcag aaggtcggtc cttctgacga cactcctaga
    11221 aggggaccag agagcctgcc agtgggacac cacgacatca tcaccgacgt cgctactttc
    11281 cagactacgc aaggattcat tgtgacagca agcagggatg gtatcgttaa agtctggaaa
    11341 taacgccatt aacctgatgt tctggggaat ataagcttgc ggccgcactc gagcaccacc
    11401 accaccacca ctgacatgcg gtaccaagct tgtcgagaag tactagagga tcataatcag
    11461 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
    11521 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
    11581 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    11641 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct
    11701 tgagcctaga agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
    11761 ccgctgagca ataactatca taacccctag ggtataccca tctaattggc ggagtttaaa
    11821 cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga taattaaaat
    11881 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
    11941 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgacg gatcccggtc
    12001 cgaaaccatg gagggttcta agaccagcaa caactcgact atgcaagtgt ccttcgtgtg
    12061 tcagagatgt agccagcctt tgaaattgga tacgagcttc aagatcctgg acagggtcac
    12121 catccaggag ctgactgctc ctctgctgac cactgctcag gccaagcccg gtgaaaccca
    12181 ggaggaagag actaacagcg gcgaagagcc cttcatcgaa acccctcgcc aggacggagt
    12241 gtctcgccgt ttcatccctc ccgctagaat gatgtctacc gagtcagcca actccttcac
    12301 tctgatcggc gaggccagcg acggtggtac tatggagaac ctgtctagga gactgaaggt
    12361 cactggcgac ctgttcgaca tcatgtcagg acagaccgac gtggaccacc ccctgtgcga
    12421 agagtgcacc gacactctgc tggaccagct ggacacccag ctgaacgtca ctgagaacga
    12481 atgccagaac tacaagcgtt gcctggagat cctggaacag atgaacgagg acgactccga
    12541 acagctgcaa atggagctga aggaactggc tctggaagag gaacgcctga tccaggagct
    12601 ggaagacgtg gagaagaacc gtaagatcgt cgctgagaac ctggaaaagg tgcaggctga
    12661 ggccgaacgc ctggaccagg aggaagccca gtaccagagg gagtactcag agttcaagcg
    12721 ccagcagctg gagctggacg acgaactgaa gtccgtcgag aaccagatgc gttacgctca
    12781 gactcagctg gacaagctga agaagaccaa cgtgttcaac gccactttcc acatctggca
    12841 cagcggacag ttcggcacca tcaacaactt caggctgggt agactgccat ctgtgcctgt
    12901 cgagtggaac gaaatcaacg ctgcctgggg ccagactgtc ctgctgctgc acgctctggc
    12961 caacaagatg ggactgaagt tccagcgcta ccgtctggtg ccatacggta accacagcta
    13021 cctggagtct ctgaccgaca agtcaaagga actgcctctg tactgctccg gaggtctgcg
    13081 cttcttctgg gacaacaagt tcgaccacgc tatggtggcc ttcctggact gcgtccagca
    13141 gttcaaggag gaagtcgaga agggcgaaac tcgcttctgc ctgccttacc gtatggacgt
    13201 ggagaaggga aagatcgaag acaccggcgg atcaggtggc tcctacagca tcaagaccca
    13261 gttcaactcc gaggaacagt ggactaaggc cctgaagttc atgctcacaa acctgaagtg
    13321 gggattggcg tgggtgtcat cgcagttcta caacaaataa cgccattaac ctgatgttct
    13381 ggggaatata agcttgcggc cgcactcgag caccaccacc accaccactg acatgcggta
    13441 ccaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt
    13501 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
    13561 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    13621 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    13681 tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctagaaga tccggctgct
    13741 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actatcataa
    13801 cccctagggt atacccatct aattggggta ggctgagtag gcaagatgtt ctggtatgtt
    13861 taaacaacca gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat
    13921 tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt
    13981 aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat
    14041 ttttcttcct gttatgtttt taatcaaaca tcctgccaac tccatgtgac aaaccgtcat
    14101 cttcggctac tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa
    14161 tgtttgtaat tgactgaata tcaacgctta tttgcagcct gaatggcgaa tgggacgcgc
    14221 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
    14281 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
    14341 ccaaacgtac tgcggtaaag ctcatcagcg tggtcgtgaa gaattcttag aaaaactcat
    14401 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
    14461 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
    14521 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
    14581 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
    14641 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
    14701 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
    14761 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
    14821 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
    14881 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
    14941 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
    15001 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
    15061 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
    15121 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg
    15181 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
    15241 ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    15301 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    15361 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    15421 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
    15481 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    15541 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    15601 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    15661 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    15721 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    15781 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    15841 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    15901 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    15961 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    16021 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    16081 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
    16141 caccgcatag accagccgcg taacctggca aaatcggtta cggttgagta ataaatggat
    16201 gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa atagatctaa
    16261 actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc agtccagtta
    16321 tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc attttctgaa
    16381 gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatggtaa agactatatt
    16441 cgcggcgttg tgacaattta ccgaacaact ccgcggccgg g
//
