LOCUS       sequence-374261-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Clostridium beijerinckii pfl ZTP riboswitch A26∆ OFF control with 
            sfGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Bushhouse DZ, Lucks JB
  TITLE     Tuning strand displacement kinetics enables programmable ZTP 
            riboswitch dynamic range in vivo.
  JOURNAL   Nucleic Acids Res. 2023 Mar 2:gkad110. doi: 10.1093/nar/gkad110.
  PUBMED    36864761
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Mar 2:gkad110. doi: 10.1093/nar/gkad110."
COMMENT     Sequence Label: pJBL6090
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(58..77)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        717..751
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(934..962)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
       61 gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt
      121 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt
      181 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
      241 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca
      301 ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg
      361 ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg
      421 gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc
      481 gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa
      541 tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt
      601 cagccccata cgatataagt tgtaattctc atgtttgaca gcttatcatc gataagcttc
      661 cgatggcgcg ccgagaggct ttacacttta tgcttccggc tgaattctaa agatctttga
      721 cagctagctc agtcctaggt ataatactag tatattagat attagtcata tgactgcgga
      781 agtggagtta ccacatgaag tatgactagg catattatct tatatgccac aaaaagccga
      841 ccgtctgggc aaaaaaagcc tggattgcgt cggctttttt atatggaaaa ggaggaagga
      901 tctatgagca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      961 ggtgatgtta atgggcacaa attttcttcc ggtaaaaggt
//
