LOCUS       sequence-374262-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Synthetic OFF-logic Clostridium beijerinckii pfl ZTP riboswitch with
            sfGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Bushhouse DZ, Lucks JB
  TITLE     Tuning strand displacement kinetics enables programmable ZTP 
            riboswitch dynamic range in vivo.
  JOURNAL   Nucleic Acids Res. 2023 Mar 2:gkad110. doi: 10.1093/nar/gkad110.
  PUBMED    36864761
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Mar 2:gkad110. doi: 10.1093/nar/gkad110."
COMMENT     Sequence Label: pJBL6392
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(237..265)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     862..882
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gccccagggg cttgaacgct ggctcgtcct aggtataata ctagtatatt agatattagt
       61 catatgactg acggaagtgg agttaccaca tgaagtatga ctaggcatat tatcttatat
      121 gccacaaaaa gccgaccgtc tgggcaaaaa aagcctggat tgcgtcggcg gtccaggttt
      181 tttatatgga aaaggaggaa ggatctatga gcaaaggaga agaacttttc actggagttg
      241 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtccgtggag
      301 agggtgaagg tgatgctaca aacggaaaac tcacccttaa atttatttgc actactggaa
      361 aactacctgt tccgtggcca acacttgtca ctactctgac ctatggtgtt caatgctttt
      421 cccgttatcc ggatcacatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
      481 atgtacagga acgcactata tctttcaaag atgacgggac ctacaagacg cgtgctgaag
      541 tcaagtttga aggtgatacc cttgttaatc gtatcgagtt aaagggtatt gattttaaag
      601 aagatggaaa cattcttgga cacaaactcg agtacaactt taactcacac aatgtataca
      661 tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattcgc cacaacgttg
      721 aagatggttc cgttcaacta gcagaccatt atcaacaaaa tactccaatt gggcgatggc
      781 cctgtccttt taccagacaa cccattacct gtcgacacaa tctgtccttt cgaaagatcc
      841 caacgaaaag cgtgaccaca tggtccttct tgagtttgta actgctgctg ggattacaca
      901 tggcatggat gagcttctaa caaataagga tctgaagctt gggcccgaac aaaaaactca
      961 tctcagaaga ggatctgaat agcgccgtcg accatcatcc
//
