LOCUS       sequence-374268-        4018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Component of optically-induced motor dimerization system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Lemma LM, Varghese M, Ross TD, Thomson M, Baskaran A, Dogic Z
  TITLE     Spatio-temporal patterning of extensile active stresses in 
            microtubule-based active fluids.
  JOURNAL   PNAS Nexus. 2023 Apr 12;2(5):pgad130. doi: 
            10.1093/pnasnexus/pgad130. eCollection 2023 May.
  PUBMED    37168671
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PNAS 
            Nexus."; date: "2023-04-12"; volume: "2(5):pgad130. doi"; pages: " 
            10.1093/pnasnexus/pgad130. eCollection 2023 May"
COMMENT     Sequence Label: K365-iLid
FEATURES             Location/Qualifiers
     source          1..4018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1154..1591
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     CDS             1616..1633
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(1715..2506,2507..2575))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2338..2357
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2576..2680)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2748..2766
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2895..3483
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3384..3403
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3637..3654
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3889..3911
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3976..3995
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3976..3994
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaataatttt gtttaacttt aagaaggaga tatacatatg tccgcggaac gagagattcc
       61 cgccgaggac agcatcaaag tggtctgccg attccgaccg ctgaacgaca gcgaagagaa
      121 ggccggctcc aagttcgtgg tcaagttccc caacaatgtg gaggagaact gcatatccat
      181 agcgggcaag gtgtatttgt tcgacaaggt cttcaaaccg aatgcatccc aggaaaaggt
      241 ctacaatgag gcggccaagt ccattgttac ggatgtcctg gccgggtaca atggaacgat
      301 attcgcatat ggtcagacgt cctccggaaa aacgcatacg atggagggcg tgatcgggga
      361 ctccgtaaaa cagggtatca taccacgtat cgtcaacgac attttcaatc acatctacgc
      421 gatggaggtg aacctggagt ttcacatcaa ggtctcctac tacgagatct acatggacaa
      481 gattcgagat ctgttggacg tctccaaggt gaaccttagt gtgcacgagg ataagaaccg
      541 ggtgccgtac gtcaagggcg ctacggaacg gttcgtctcg tcgccggagg atgttttcga
      601 ggtgatcgag gagggcaaat ccaatcgtca catcgctgtg acaaacatga acgagcattc
      661 ttcgcgatcc cactcagtat tccttatcaa tgtgaagcag gagaacctgg agaaccagaa
      721 gaaactatcc ggcaaactct acctggtgga tttggccggt tccgagaagg tttccaagac
      781 tggagcggag ggaaccgttc ttgatgaagc caagaacatc aacaagtcgc tgtcggcctt
      841 gggcaacgta atttctgccc tggcggacgg aaacaaaacg cacatcccct accgtgattc
      901 caagctaacg cgcatcctgc aggagtcgct gggaggcaac gcacgcacaa ccatcgtcat
      961 ctgctgctct ccagccagtt tcaacgaatc tgaaacgaag tcaacgctgg acttcggtcg
     1021 tagagccaag acagtgaaga acgtggtctg cgttaacgag gagcttactg ccgaggaatg
     1081 gaagcgacgc tatgaaaagg agaaggaaaa gaacgcccga ctaaagggta agggtagtgg
     1141 tagtggggag tttctggcaa ccacactgga acggatcgag aaaaatttcg tgattactga
     1201 tccgagactg cctgacaacc caatcatttt tgcgagcgat tccttcctgc agctgacaga
     1261 atattctcgg gaagagatcc tggggcgcaa ttgccgtttt ctgcagggac ccgagacaga
     1321 ccgtgccact gttcggaaaa tcagagatgc tattgacaac cagactgaag tgaccgttca
     1381 gctgatcaat tataccaaga gcggcaagaa gttctggaac gtgttccacc tgcagccgat
     1441 gcgcgattat aagggcgacg tccagtactt cattggcgtg cagctggatg gcaccgaacg
     1501 tcttcatggc gccgctgagc gtgaggcggt ctgcctgatc aaaaagacag cctttcagat
     1561 tgctgaggca gcgaacgacg aaaattactt tggaagcggg tctgagacta gtgggcacca
     1621 ccatcaccat cactgacaag cttatcgatg ataagctgtc aaacatgaga attaaatcaa
     1681 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1741 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1801 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     1861 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1921 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1981 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2041 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2101 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2161 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     2221 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     2281 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     2341 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     2401 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     2461 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     2521 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     2581 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     2641 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     2701 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2761 cgaggccctt tcgtcttcaa gaataaaagg atctaggtga agatcctttt tgataatctc
     2821 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2881 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2941 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3001 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     3061 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3121 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3181 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3241 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3301 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3361 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3421 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3481 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3541 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3601 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3661 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3721 ggaagatctt ccggaagatc ttcctatggt gcactctcag tacaatctgc tctgatgcgc
     3781 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     3841 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3901 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcccagcgat tcgaacttct
     3961 gatagacttc gaaattaata cgactcacta tagggagacc acaacggttt ccctctag
//
