LOCUS       sequence-374272-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Clostridium beijerinckii pfl ZTP riboswitch with sfGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Bushhouse DZ, Lucks JB
  TITLE     Tuning strand displacement kinetics enables programmable ZTP 
            riboswitch dynamic range in vivo.
  JOURNAL   Nucleic Acids Res. 2023 Mar 2:gkad110. doi: 10.1093/nar/gkad110.
  PUBMED    36864761
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Mar 2:gkad110. doi: 10.1093/nar/gkad110."
COMMENT     Sequence Label: pJBL3907
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(58..77)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        716..750
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(934..962)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
       61 gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt
      121 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgctg acactcagtt
      181 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc
      241 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac
      301 cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt
      361 taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg
      421 ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg
      481 ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat
      541 cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
      601 agccccatac gatataagtt gtaattctca tgtttgacag cttatcatcg ataagcttcc
      661 gatggcgcgc cgagaggctt tacactttat gcttccggct gaattctaaa gatctttgac
      721 agctagctca gtcctaggta taatactagt atattagata ttagtcatat gactgacgga
      781 agtggagtta ccacatgaag tatgactagg catattatct tatatgccac aaaaagccga
      841 ccgtctgggc aaaaaaagcc tggattgcgt cggctttttt atatggaaaa ggaggaagga
      901 tctatgagca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      961 ggtgatgtta atgggcacaa aatttcctcc tgtggatacc
//
