LOCUS       sequence-374273-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Synthetic OFF-logic Clostridium beijerinckii pfl ZTP riboswitch with
            sfGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Bushhouse DZ, Lucks JB
  TITLE     Tuning strand displacement kinetics enables programmable ZTP 
            riboswitch dynamic range in vivo.
  JOURNAL   Nucleic Acids Res. 2023 Mar 2:gkad110. doi: 10.1093/nar/gkad110.
  PUBMED    36864761
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Mar 2:gkad110. doi: 10.1093/nar/gkad110."
COMMENT     Sequence Label: pJBL6392
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(48..67)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        706..740
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(932..960)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 atcacgaatc tgacgctcaa atcagtgtgc gaacccgaca ggactataaa gataccaggc
       61 gtttcccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
      121 attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc
      181 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt
      241 atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc
      301 agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa
      361 ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt
      421 tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc
      481 aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat
      541 atttctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac
      601 gatataagtt gtaattctca tgtttgacag cttatcatcg ataagcttcc gatggcgcgc
      661 cgagaggctt tacactttat gcttccggct gaattctaaa gatctttgac agctagctca
      721 gtcctaggta taatactagt atattagata ttagtcatat gactgacgga agtggagtta
      781 ccacatgaag tatgactagg catattatct tatatgccac aaaaagccga ccgtctgggc
      841 aaaaaaagcc tggattgcgt cggcggtcca ggttttttat atggaaaagg aggaaggatc
      901 tatgagcaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      961 tgatgttaat gggcacaaat tttcttcccc tatgatgttg
//
