LOCUS       sequence-374279-       10910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of AKT1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10910)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-AKT1_IDG-K
FEATURES             Location/Qualifiers
     source          1..10910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2013..2034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2049..2054,2055..2072,2073..2079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2087..2103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2092..2114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2111..2127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2111..2127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2146..2166
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2148..2166
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2194..2420
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2421..2601
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2648..2773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3266..3499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3684..3728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3995..4111
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4145..4165
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4165..5376
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5213..5232
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5380..5400)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5401..5410
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5413..6183
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6190..6231
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6232..6256
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6262..7701
                     /codon_start=1
                     /gene="AKT1"
                     /product="human serine/threonine protein kinase activated 
                     by growth factors"
                     /label=Akt1
                     /translation="MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDV
                     DQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQ
                     TVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGK
                     VILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDR
                     LCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLM
                     LDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMY
                     EMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDA
                     KEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSM
                     ECVDSERRPHFPQFSYSASGTA"
     protein_bind    complement(7709..7733)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7763..8351
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7816..7836)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8234..8245)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8365..8864
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8400..8423)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8797..8816
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8885..9484
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8885..8904)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9381..9401
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9622..9855
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9927..10048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9964..9983)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10018..10037
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10088..10223
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10243..10262)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10244..10262)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10272..10289)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10272..10288)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10281..10303)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10430..10885
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10517..10536)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10727..10748
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     1921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     1981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2101 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     2161 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     2221 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     2281 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     2341 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     2401 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     2461 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2521 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2581 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     2641 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     2701 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     2761 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     2821 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     2881 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     2941 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     3001 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     3061 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     3121 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     3181 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     3241 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     3301 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     3361 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     3421 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     3481 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     3541 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     3601 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     3661 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     3721 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     3781 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     3841 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     3901 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     3961 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
     4021 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4081 tacaaaaaca aattacaaaa ttcaaaattt tatcgtccag tgtggtggaa ttctgcagat
     4141 atcacaactt tgtatagaaa agttggcctc cgcgccgggt tttggcgcct cccgcgggcg
     4201 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc
     4261 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     4321 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     4381 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     4441 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     4501 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     4561 tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     4621 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     4681 ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga
     4741 agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc
     4801 aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg
     4861 gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     4921 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     4981 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     5041 tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg
     5101 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     5161 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     5221 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     5281 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc
     5341 gctaaattct ggccgttttt ggcttttttg ttagacggcc aacttttgta tacaaagtgg
     5401 gccaccatgg gaatcccgct gctgaacgct aaacagattc tgggacagct ggacggcggg
     5461 agcgtggcag tcctgcctgt ggtcgactcc accaatcagt acctgctgga tcgaatcggc
     5521 gagctgaaga gtggggatgc ttgcattgca gaatatcagc aggcagggag aggaagcaga
     5581 gggaggaaat ggttctctcc ttttggagct aacctgtacc tgagtatgtt ttggcgcctg
     5641 aagcggggac cagcagcaat cggcctgggc ccggtcatcg gaattgtcat ggcagaagcg
     5701 ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc ccaatgacct gtatctgcag
     5761 gatagaaagc tggcaggcat cctggtggag ctggccggaa taacaggcga tgctgcacag
     5821 atcgtcattg gcgccgggat taacgtggct atgaggcgcg tggaggaaag cgtggtcaat
     5881 cagggctgga tcacactgca ggaagcaggg attaacctgg acaggaatac tctggccgct
     5941 atgctgatcc gagagctgcg ggcagccctg gaactgttcg agcaggaagg cctggctcca
     6001 tatctgtcac ggtgggagaa gctggataac ttcatcaata gacccgtgaa gctgatcatt
     6061 ggggacaaag agattttcgg gattagccgg gggattgata aacagggagc cctgctgctg
     6121 gaacaggacg gagttatcaa accctggatg ggcggagaaa tcagtctgcg gtctgccgaa
     6181 aaggctagcg gcaagcccat ccccaacccc ctgctgggcc tggacagcac cacaagtttg
     6241 tacaaaaaag caggctccac catgagcgac gtggctattg tgaaggaggg ttggctgcac
     6301 aaacgagggg agtacatcaa gacctggcgg ccacgctact tcctcctcaa gaatgatggc
     6361 accttcattg gctacaagga gcggccgcag gatgtggacc aacgtgaggc tcccctcaac
     6421 aacttctctg tggcgcagtg ccagctgatg aagacggagc ggccccggcc caacaccttc
     6481 atcatccgct gcctgcagtg gaccactgtc atcgaacgca ccttccatgt ggagactcct
     6541 gaggagcggg aggagtggac aaccgccatc cagactgtgg ctgacggcct caagaagcag
     6601 gaggaggagg agatggactt ccggtcgggc tcacccagtg acaactcagg ggctgaagag
     6661 atggaggtgt ccctggccaa gcccaagcac cgcgtgacca tgaacgagtt tgagtacctg
     6721 aagctgctgg gcaagggcac tttcggcaag gtgatcctgg tgaaggagaa ggccacaggc
     6781 cgctactacg ccatgaagat cctcaagaag gaagtcatcg tggccaagga cgaggtggcc
     6841 cacacactca ccgagaaccg cgtcctgcag aactccaggc accccttcct cacagccctg
     6901 aagtactctt tccagaccca cgaccgcctc tgctttgtca tggagtacgc caacgggggc
     6961 gagctgttct tccacctgtc ccgggagcgt gtgttctccg aggaccgggc ccgcttctat
     7021 ggcgctgaga ttgtgtcagc cctggactac ctgcactcgg agaagaacgt ggtgtaccgg
     7081 gacctcaagc tggagaacct catgctggac aaggacgggc acattaagat cacagacttc
     7141 gggctgtgca aggaggggat caaggacggt gccaccatga agaccttttg cggcacacct
     7201 gagtacctgg cccccgaggt gctggaggac aatgactacg gccgtgcagt ggactggtgg
     7261 gggctgggcg tggtcatgta cgagatgatg tgcggtcgcc tgcccttcta caaccaggac
     7321 catgagaagc tttttgagct catcctcatg gaggagatcc gcttcccgcg cacgcttggt
     7381 cccgaggcca agtccttgct ttcagggctg ctcaagaagg accccaagca gaggcttggc
     7441 gggggctccg aggacgccaa ggagatcatg cagcatcgct tctttgccgg tatcgtgtgg
     7501 cagcacgtgt acgagaagaa gctcagccca cccttcaagc cccaggtcac gtcggagact
     7561 gacaccaggt attttgatga ggagttcacg gcccagatga tcaccatcac accacctgac
     7621 caagatgaca gcatggagtg tgtggacagc gagcgcaggc cccacttccc ccagttctcc
     7681 tactcggcca gcggcacggc ctgaatccac ccagctttct tgtacaaagt ggtgatatcc
     7741 agcacagtgg cggccgctcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     7801 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     7861 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7921 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7981 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     8041 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     8101 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     8161 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     8221 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     8281 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     8341 cgcctccccg cctggaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     8401 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     8461 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     8521 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     8581 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     8641 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     8701 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     8761 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     8821 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     8881 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     8941 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     9001 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     9061 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     9121 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     9181 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     9241 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     9301 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9361 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9421 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9481 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
     9541 tcgagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     9601 ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc
     9661 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9721 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9781 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9841 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     9901 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     9961 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    10021 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
    10081 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10141 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    10201 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
    10261 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
    10321 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
    10381 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
    10441 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    10501 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    10561 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    10621 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    10681 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    10741 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    10801 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    10861 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
//
