LOCUS       sequence-37430-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2308 (=HL 716 + pHL 814)
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(217..303)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    493..511
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    518..536
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    759..777
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    784..802
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gactgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
       61 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      121 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      181 tcgccgcagc cgaacgccct aggtctaggg cggcggattt gtcctactca ggagagcgtt
      241 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      301 ttgatgcctc tagcacgcgt accatgggcc ctttgagcct ggcttatcgc cgggcttttt
      361 tatggcaaaa aaaagcggat cctggagatc cgcaaaagtt cacgttggct ttagttattc
      421 gagttgagaa actctcgaaa cgggcagtga cttcaagggt taaaagaggt gccgctccgt
      481 ttcccgggag tatctctatc actgataggg atgtcaatct ctatcactga tagggagaat
      541 tcactgctca caagaaaaaa ggcacgtcat ctgacgtgcc ttttttattt gtactaccct
      601 gtacgattaa agcttctgga taaggattat ccaattctaa aaaaaaagcc cggacgactg
      661 ttcgggcttg tctttttata tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa
      721 taaaatctgt gacaataaag gcatataacc cgggagtatc tctatcactg atagggatgt
      781 caatctctat cactgatagg gaggatcctg agcggataca ta
//
