LOCUS       sequence-374317-        7876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent human androgen receptor (fused to EGFP) with deleted 
            polyglutamine region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Stenoien DL, Cummings CJ, Adams HP, Mancini MG, Patel K, DeMartino 
            GN, Marcelli M, Weigel NL, Mancini MA
  TITLE     Polyglutamine-expanded androgen receptors form aggregates that 
            sequester heat shock proteins, proteasome components and SRC-1, and 
            are suppressed by the HDJ-2 chaperone.
  JOURNAL   Hum Mol Genet. 1999 May . 8(5):731-41.
  PUBMED    10196362
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 1999 May . 8(5):731-41."
COMMENT     Sequence Label: pEGFP-C1-AR Q0
FEATURES             Location/Qualifiers
     source          1..7876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4664..4785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4701..4720)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4755..4774
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4792..5247)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4929..4950)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5141..5160
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5274..5378
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5380..5737
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5380..5571
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5403..5423)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5588..5723
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5772..6566
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5772..6566
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5826..5845)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6436..6455
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6754..6773)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6798..6845
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7174..7762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7663..7682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1381 atggaagtgc agttagggct gggaagggtc taccctcggc cgccgtccaa gacctaccga
     1441 ggagctttcc agaatctgtt ccagagcgtg cgcgaagtga tccagaaccc gggccccagg
     1501 cacccagagg ccgcgagcgc agcacctccc ggcgccagtt tgctgctgct cgagactagc
     1561 cccaggcagc agcagcagca gcagggtgag gatggttctc cccaagccca tcgtagaggc
     1621 cccacaggct acctggtcct ggatgaggaa cagcaacctt cacagccgca gtcggccctg
     1681 gagtgccacc ccgagagagg ttgcgtccca gagcctggag ccgccgtggc cgccagcaag
     1741 gggctgccgc agcagctgcc agcacctccg gacgaggatg actcagctgc cccatccacg
     1801 ttgtccctgc tgggccccac tttccccggc ttaagcagct gctccgctga ccttaaagac
     1861 atcctgagcg aggccagcac catgcaactc cttcagcaac agcagcagga agcagtatcc
     1921 gaaggcagca gcagcgggag agcgagggag gcctcggggg ctcccacttc ctccaaggac
     1981 aattacttag ggggcacttc gaccatttct gacaacgcca aggagttgtg taaggcagtg
     2041 tcggtgtcca tgggcctggg tgtggaggcg ttggagcatc tgagtccagg ggaacagctt
     2101 cggggggatt gcatgtacgc cccacttttg ggagttccac ccgctgtgcg tcccactcct
     2161 tgtgccccat tggccgaatg caaaggttct ctgctagacg acagcgcagg caagagcact
     2221 gaagatactg ctgagtattc ccctttcaag ggaggttaca ccaaagggct agaaggcgag
     2281 agcctaggct gctctggcag cgctgcagca gggagctccg ggacacttga actgccgtct
     2341 accctgtctc tctacaagtc cggagcactg gacgaggcag ctgcgtacca gagtcgcgac
     2401 tactacaact ttccactggc tctggccgga ccgccgcccc ctccgccgcc tccccatccc
     2461 cacgctcgca tcaagctgga gaacccgctg gactacggca gcgcctgggc ggctgcggcg
     2521 gcgcagtgcc gctatgggga cctggcgagc ctgcatggcg cgggtgcagc gggacccggt
     2581 tctgggtcac cctcagccgc cgcttcctca tcctggcaca ctctcttcac agccgaagaa
     2641 ggccagttgt atggaccgtg tggtggtggt gggggtggtg gcggcggcgg cggcggcggc
     2701 ggcggcggcg gcggcggcgg cggcggcggc gaggcgggag ctgtagcccc ctacggctac
     2761 actcggcccc ctcaggggct ggcgggccag gaaagcgact tcaccgcacc tgatgtgtgg
     2821 taccctggcg gcatggtgag cagagtgccc tatcccagtc ccacttgtgt caaaagcgaa
     2881 atgggcccct ggatggatag ctactccgga ccttacgggg acatgcgttt ggagactgcc
     2941 agggaccatg ttttgcccat tgactattac tttccacccc agaagacctg cctgatctgt
     3001 ggagatgaag cttctgggtg tcactatgga gctctcacat gtggaagctg caaggtcttc
     3061 ttcaaaagag ccgctgaagg gaaacagaag tacctgtgcg ccagcagaaa tgattgcact
     3121 attgataaat tccgaaggaa aaattgtcca tcttgtcgtc ttcggaaatg ttatgaagca
     3181 gggatgactc tgggagcccg gaagctgaag aaacttggta atctgaaact acaggaggaa
     3241 ggagaggctt ccagcaccac cagccccact gaggagacaa cccagaagct gacagtgtca
     3301 cacattgaag gctatgaatg tcagcccatc tttctgaatg tcctggaagc cattgagcca
     3361 ggtgtagtgt gtgctggaca cgacaacaac cagcccgact cctttgcagc cttgctctct
     3421 agcctcaatg aactgggaga gagacagctt gtacacgtgg tcaagtgggc caaggccttg
     3481 cctggcttcc gcaacttaca cgtggacgac cagatggctg tcattcagta ctcctggatg
     3541 gggctcatgg tgtttgccat gggctggcga tccttcacca atgtcaactc caggatgctc
     3601 tacttcgccc ctgatctggt tttcaatgag taccgcatgc acaagtcccg gatgtacagc
     3661 cagtgtgtcc gaatgaggca cctctctcaa gagtttggat ggctccaaat caccccccag
     3721 gaattcctgt gcatgaaagc actgctactc ttcagcatta ttccagtgga tgggctgaaa
     3781 aatcaaaaat tctttgatga acttcgaatg aactacatca aggaactcga tcgtatcatt
     3841 gcatgcaaaa gaaaaaatcc cacatcctgc tcaagacgct tctaccagct caccaagctc
     3901 ctggactccg tgcagcctat tgcgagagag ctgcatcagt tcacttttga cctgctaatc
     3961 aagtcacaca tggtgagcgt ggactttccg gaaatgatgg cagagatcat ctctgtgcaa
     4021 gtgcccaaga tcctttctgg gaaagtcaag cccatctatt tccacaccca gtgaagcatt
     4081 ggaaacccta tttccccacc ccagctcatg ccccctttca gatgtcttct gcctgttata
     4141 actctgcact actcctctgc agtgccttgg ggaatttcct ctattgatgt acagtctgtc
     4201 atgaacatgt tcctgaattc tatttgctgg gctttttttt tctctttctc tcctttcttt
     4261 ttcttcttcc ctccctatct aaccctccca tggcaccttc agactttgct tcccattgtg
     4321 gctcctatct gtgttttgaa tggtgttgta tgcctttaaa tctgtgatga tcctcatatg
     4381 gcccagtgtc aagttgtgct tgtttacagc actactctgt gccagccaca caaacgttta
     4441 cttatcttat gccacgggaa gtttagagag ctaagattat ctggggaaat caaaacaaaa
     4501 acaagcaaac aaaaaaaaaa gaaaaacgga attcagatcc accggatcta gataactgat
     4561 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     4621 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     4681 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4741 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa
     4801 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     4861 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     4921 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     4981 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     5041 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     5101 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     5161 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     5221 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5281 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5341 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     5401 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5461 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5521 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5581 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5641 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5701 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     5761 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5821 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5881 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5941 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     6001 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     6061 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     6121 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     6181 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     6241 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     6301 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     6361 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     6421 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     6481 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     6541 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     6601 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6661 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6721 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     6781 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     6841 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     6901 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     6961 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     7021 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7081 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7141 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7201 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7261 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7321 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7381 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7441 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7501 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7561 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7621 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7681 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7741 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7801 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7861 tattaccgcc atgcat
//
