LOCUS       sequence-374318-       13202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TTBK2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13202)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 13202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-TTBK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..13202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4170..4190
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4190..5401
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5238..5257
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5405..5425)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5426..5435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5438..6208
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6215..6256
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6257..6281
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10026..10050)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10080..10668
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10133..10153)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10551..10562)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10682..11181
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10717..10740)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11114..11133
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11202..11801
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11202..11221)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11698..11718
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             11939..12172
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12244..12365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12281..12300)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12335..12354
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12405..12540
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12560..12579)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12561..12579)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12589..12606)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12589..12605)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12598..12620)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12747..13175
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12834..12853)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13044..13065
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcac aactttgtat agaaaagttg gcctccgcgc
     4201 cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac
     4261 gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc
     4321 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg
     4381 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc
     4441 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag
     4501 gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg
     4561 tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt
     4621 tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta
     4681 gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg
     4741 cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa
     4801 caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg
     4861 gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt
     4921 tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc
     4981 cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc
     5041 cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt
     5101 tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc
     5161 ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac
     5221 ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt
     5281 gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt
     5341 tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga
     5401 cggccaactt ttgtatacaa agtgggccac catgggaatc ccgctgctga acgctaaaca
     5461 gattctggga cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa
     5521 tcagtacctg ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata
     5581 tcagcaggca gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct
     5641 gtacctgagt atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt
     5701 catcggaatt gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa
     5761 atggcccaat gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc
     5821 cggaataaca ggcgatgctg cacagatcgt cattggcgcc gggattaacg tggctatgag
     5881 gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag cagggattaa
     5941 cctggacagg aatactctgg ccgctatgct gatccgagag ctgcgggcag ccctggaact
     6001 gttcgagcag gaaggcctgg ctccatatct gtcacggtgg gagaagctgg ataacttcat
     6061 caatagaccc gtgaagctga tcattgggga caaagagatt ttcgggatta gccgggggat
     6121 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
     6181 agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct
     6241 gggcctggac agcaccacaa gtttgtacaa aaaagcaggc ttagccacca tgagtggggg
     6301 aggagagcag ccggatatcc tgagtgttgg aatcctagtg aaagaaagat ggaaagtgtt
     6361 gagaaagatt gggggtgggg gctttggaga aatttacgat gccttggaca tgctcaccag
     6421 ggaaaatgtt gcactgaagg tggaatcagc tcaacaacca aaacaagttc tgaaaatgga
     6481 agttgctgtt ttgaaaaagc tgcaagggaa agaccatgtt tgtagattta ttggctgtgg
     6541 gaggaatgat cgattcaact atgtggtcat gcagttgcag ggtcggaatc tggcagatct
     6601 tcgccgtagc cagtcccgag gcacattcac cattagtacc actctccggc tgggtagaca
     6661 gattttggag tctattgaaa gcattcattc tgtgggattc ttgcatcgag acatcaaacc
     6721 gtcgaacttc gctatgggtc gctttcctag tacatgtagg aaatgttaca tgcttgattt
     6781 tggcttggct cgacaattta ccaattcctg tggtgacgtc agaccacctc gagctgtggc
     6841 aggttttcga gggacagttc gttatgcatc aatcaacgca catcggaaca gggaaatggg
     6901 aagacatgat gacctttggt ccttattcta catgttggtg gagtttgtgg ttggtcagct
     6961 gccctggaga aaaataaagg acaaggagca agtaggctct attaaggaga gatatgacca
     7021 caggctcatg ttgaaacatc tccctccaga attcagcatc tttctagacc atatctcttc
     7081 tttggattat tttacaaaac cagactacca gcttcttaca tccgtgtttg acaatagcat
     7141 caagactttt ggagtaattg agagtgaccc ttttgactgg gagaagactg gaaatgatgg
     7201 ctccctaaca accaccacta cttctaccac acctcagttg cacactcgct tgacccctgc
     7261 tgcaattgga attgccaatg ctactcacat ccctggagac ttgcttcgag aaaatacaga
     7321 tgaggtattt ccagatgaac agcttagcga tggagaaaat ggcatccctg ttggtgtgtc
     7381 accagataaa ttgcctggat ctctgggaca cccccgtccc caggagaagg atgtttggga
     7441 agagatggat gccaacaaaa acaagataaa gcttggaatt tgtaaggctg ctactgaaga
     7501 ggagaacagc catggccagg caaatggtct tctcaatgct ccaagccttg ggtcaccaat
     7561 tcgtgtccgc tcagagatta ctcagccaga cagagatatt ccactggtgc gaaagttacg
     7621 ttccattcac agctttgagc tggaaaaacg tctgaccctg gagccaaagc cagacactga
     7681 caagttcctt gagacctgcc tggagaaaat gcagaaagat accagtgcag gaaaagaatc
     7741 tattctccct gctctgctgc ataagccttg cgttcctgct gtgtcccgta ctgaccacat
     7801 ctggcactat gatgaagaat atcttccaga tgcctccaag cctgcttctg ccaacacccc
     7861 tgagcaggca gatggtggtg gcagcaatgg atttatagct gttaacctga gctcttgcaa
     7921 gcaagaaatt gattccaaag aatgggtgat tgtggacaag gagcaggacc ttcaggattt
     7981 taggacaaat gaggctgtag gacataaaac aactggaagt ccttctgatg aggagcctga
     8041 agtacttcaa gtcctggagg catcacctca agatgaaaag ctccagttag gtccttgggc
     8101 agaaaatgat catttaaaga aggaaacctc aggtgtggtc ttagcacttt ctgcagaggg
     8161 tcctcctact gctgcttcag aacaatatac agataggctg gaactccagc ctggagctgc
     8221 tagtcagttt attgcagcga cgcccacaag tctaatggag gcgcaggcag aaggacccct
     8281 tacagcgatt acaattccta gaccttctgt ggcatctaca cagtcaactt caggaagctt
     8341 tcactgtggt cagcagccag agaagaaaga tcttcagccc atggagccca ctgtggaact
     8401 ttactctcca agggaaaact tctctggctt ggttgtgaca gagggtgaac ctcctagtgg
     8461 aggaagcaga acagatttgg ggcttcagat agatcacatt ggtcatgaca tgttacccaa
     8521 cattagagaa agtaacaaat ctcaagacct gggaccaaaa gaacttcctg atcataatag
     8581 actggttgtg agagaatttg aaaatctccc tggggaaact gaagagaaaa gcatcctttt
     8641 agagtcagat aatgaagatg agaagttaag tagagggcag cattgtattg agatctcctc
     8701 tctcccagga gatttggtaa ttgtggaaaa ggatcactca gctactactg aacctcttga
     8761 tgtgacaaaa acacagactt ttagtgtggt gccaaatcaa gacaaaaata atgagataat
     8821 gaagcttctg acagttggaa cttcagaaat ttcttccaga gacattgacc cacatgttga
     8881 aggtcagata ggccaagtgg cagaaatgca aaaaaataag atatctaagg atgatgacat
     8941 catgagtgaa gacttgccag gtcatcaagg agacctctct acttttttgc accaagaggg
     9001 caagagagag aaaatcaccc ctagaaatgg agaactattt cattgtgttt cagagaatga
     9061 acatggtgcc ccaacccgga aggatatggt taggtcatcc tttgtaacta gacacagccg
     9121 aatccctgtt ttagcacaag agatagactc aactttggaa tcatcctctc cagtttctgc
     9181 aaaagaaaag ctcctccaaa agaaagccta tcagccagac ctagtcaagc ttctggtgga
     9241 aaaaagacaa ttcaagtcct tccttggcga cctctcaagt gcctctgata aattgctaga
     9301 ggagaaacta gctactgttc ctgctccctt ttgtgaggag gaagtgctca ctcccttttc
     9361 aagactgaca gtagattctc acctgagtag gtcagctgaa gatagctttc tgtcacccat
     9421 catctcccag tctagaaaga gcaaaattcc aaggccagtt tcatgggtca acacagatca
     9481 ggtcaatagc tcaacttcgt ctcagttctt tcctcggcca ccaccaggaa agccacccac
     9541 gaggcctgga gtagaagcca ggctacgcag atataaagtc ctagggagta gtaactccga
     9601 ctcagacctt ttctcccgcc tggcccaaat tcttcaaaat ggatctcaga aaccccggag
     9661 cactactcag tgcaagagtc caggatctcc tcacaatcca aaaacaccac ccaagagtcc
     9721 agttgtccct cgcaggagtc ccagtgcctc tcctcgaagc tcatccttgc ctcgcacgtc
     9781 tagttcctca ccatctaggg ctggacggcc ccaccatgac cagaggagtt cgtccccaca
     9841 tctggggaga agcaagtcac ctcccagcca ctcaggatct tcctcctcca ggaggtcctg
     9901 ccaacaggag cattgcaaac ccagcaagaa tggcctgaaa ggatccggca gcctccacca
     9961 ccactcagcc agcactaaaa ccccccaagg gaagagtaag ccagccagta aactcagcag
    10021 ataaaaccca gctttcttgt acaaagtggt gatatccagc acagtggcgg ccgctcgaca
    10081 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
    10141 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
    10201 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
    10261 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
    10321 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
    10381 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
    10441 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
    10501 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
    10561 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
    10621 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctac
    10681 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
    10741 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
    10801 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
    10861 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
    10921 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
    10981 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
    11041 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
    11101 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
    11161 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca
    11221 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
    11281 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
    11341 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
    11401 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
    11461 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
    11521 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
    11581 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
    11641 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
    11701 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
    11761 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
    11821 agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt acctttaaga
    11881 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
    11941 gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
    12001 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    12061 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    12121 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
    12181 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
    12241 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    12301 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    12361 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
    12421 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    12481 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    12541 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
    12601 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
    12661 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
    12721 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
    12781 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    12841 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    12901 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    12961 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    13021 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    13081 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    13141 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
    13201 tt
//
