LOCUS       sequence-374351-       11073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ecDHFR DD-mCherry, an inducible ecRESCUE RNA base editor: 
            TMP-induced ecDHFR DD expression allows reversible control of the 
            expression of dRanCas13b-ADAR2-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11073)
  AUTHORS   Wang Y, Li G, Li X, Wang Y, Huang X, Hu X, Gao J
  TITLE     ecRESCUE: a novel ecDHFR-regulated RESCUE system with reduced RNA 
            off-targeting activity.
  JOURNAL   Cell Commun Signal. 2021 Jul 31;19(1):81. doi: 
            10.1186/s12964-021-00759-2.
  PUBMED    34332602
REFERENCE   2  (bases 1 to 11073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Commun
            Signal."; date: "2021-07-31"; volume: "19(1):81. doi"; pages: " 
            10.1186/s12964-021-00759-2"
COMMENT     Sequence Label: 3'-ecRESCUE-mCherry
FEATURES             Location/Qualifiers
     source          1..11073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      917..926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5414..5440
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             5441..6148
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5586..5604)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5854..5873
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6149..6619
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILER"
     primer_bind     complement(6664..6681)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6670..6894
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6940..7368
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7027..7046)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7237..7258
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7377..7397)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7382..7711
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7562..7697
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7624..7643
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7778..8572
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7832..7851)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8442..8461
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8746..8867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8783..8802)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8837..8856
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8916..8932)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8916..8932)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8929..8951)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8940..8956
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8964..8970,8971..8988,8989..8994))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9009..9030
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9147..9164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9318..9906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9398..9417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10077..10868,10869..10937))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10700..10719
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10938..11042)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttcag cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatggagaa gcccctgctg cctaacgtgt ataccctgaa
      961 gcacaagttc ttttggggcg ccttcctgaa tatcgcccgg cacaacgcct ttatcaccat
     1021 ctgccacatc aatgagcagc tgggcctgaa aacccccagc aacgacgata agatcgtgga
     1081 cgtggtgtgc gagacatgga acaatatcct gaacaatgac cacgatctgc tgaagaagtc
     1141 ccagctgacc gagctgatcc tgaagcactt cccttttctg acagccatgt gctaccaccc
     1201 ccctaagaag gagggcaaga agaagggcca ccagaaggag cagcagaagg agaaggagag
     1261 cgaggcacag tcccaggcag aggccctgaa cccatccaag ctgatcgagg ccctggagat
     1321 cctggtgaat cagctgcact ctctggcaaa ctactatagc gcctataagc acaagaagcc
     1381 cgacgccgag aaggatatct tcaagcacct gtataaggcc ttcgacgcct ctctgagaat
     1441 ggtgaaggag gattataagg cccacttcac cgtgaatctg acaagggact ttgcccacct
     1501 gaaccgcaag ggcaagaaca agcaggacaa tcccgacttc aacagataca gattcgagaa
     1561 ggatggcttc tttaccgagt ctggcctgct gttctttaca aatctgtttc tggacaagag
     1621 ggatgcctat tggatgctga agaaggtgtc cggcttcaag gcctctcaca agcagcgcga
     1681 gaagatgacc acagaggtgt tttgcaggag ccgcatcctg ctgcccaagc tgaggctgga
     1741 gtcccgctac gaccacaacc agatgctgct ggatatgctg tctgagctga gcagatgtcc
     1801 taagctgctg tatgagaagc tgagcgagga gaataagaag cacttccagg tggaggccga
     1861 cggctttctg gatgagatcg aggaggagca gaacccattc aaggacaccc tgatccggca
     1921 ccaggataga ttcccctact ttgccctgag gtatctggac ctgaatgagt ccttcaagtc
     1981 tatccgcttt caggtggatc tgggcacata ccactattgt atctacgaca agaagatcgg
     2041 cgatgagcag gagaagaggc acctgacccg cacactgctg tccttcggcc ggctgcagga
     2101 ctttaccgag atcaacagac cccaggagtg gaaggccctg accaaggacc tggattacaa
     2161 ggagacatcc aatcagcctt tcatctctaa gaccacacca cactatcaca tcaccgacaa
     2221 caagatcggc tttcggctgg gcacaagcaa ggagctgtac ccctccctgg agatcaagga
     2281 tggcgccaat agaatcgcca agtaccctta taactccggc ttcgtggccc acgcctttat
     2341 ctctgtgcac gagctgctgc ctctgatgtt ctaccagcac ctgaccggca agagcgagga
     2401 cctgctgaag gagacagtgc ggcacatcca gagaatctat aaggacttcg aggaggagcg
     2461 gatcaatacc atcgaggatc tggagaaggc aaaccagggc agactgccac tgggagcctt
     2521 tcccaagcag atgctgggcc tgctgcagaa taagcagcct gatctgtccg agaaggccaa
     2581 gatcaagatc gagaagctga tcgccgagac aaagctgctg tctcacaggc tgaacacaaa
     2641 gctgaagagc tccccaaagc tgggcaagcg gagagagaag ctgatcaaga caggcgtgct
     2701 ggccgactgg ctggtgaagg atttcatgcg ctttcagccc gtggcctacg acgcccagaa
     2761 tcagcctatc aagtctagca aggccaactc caccgagttc tggtttatca ggcgcgccct
     2821 ggccctgtac ggaggagaga agaataggct ggagggctat ttcaagcaga ccaacctgat
     2881 cggcaacaca aatccacacc ccttcctgaa caagtttaat tggaaggcct gcaggaatct
     2941 ggtggatttc taccagcagt atctggagca gcgcgagaag tttctggagg ccatcaagaa
     3001 ccagccatgg gagccctacc agtattgcct gctgctgaag atccctaagg agaacaggaa
     3061 gaatctggtg aagggatggg agcagggagg aatctctctg ccacggggcc tgtttaccga
     3121 ggccatcaga gagacactgt ctgaggacct gatgctgagc aagccaatcc gcaaggagat
     3181 caagaagcac ggccgggtgg gcttcatcag cagagccatc accctgtact ttaaggagaa
     3241 gtatcaggat aagcaccaga gcttctacaa tctgtcctat aagctggagg caaaggcacc
     3301 actgctgaag cgggaggagc actacgagta ttggcagcag aacaagcctc agtctccaac
     3361 cgagagccag aggctggagc tgcacacaag cgaccgctgg aaggattacc tgctgtataa
     3421 gagatggcag cacctggaga agaagctgcg gctgtaccgg aatcaggacg tgatgctgtg
     3481 gctgatgacc ctggagctga caaagaacca cttcaaggag ctgaacctga attatcacca
     3541 gctgaagctg gagaatctgg ccgtgaacgt gcaggaggcc gatgccaagc tgaaccctct
     3601 gaatcagacc ctgcccatgg tgctgcctgt gaaggtgtac cctgccacag cctttggcga
     3661 ggtgcagtac cacaagaccc caatcaggac agtgtatatc cgcgaggagc acaccaaggc
     3721 cctgaagatg ggcaacttca aggccctggt gaaggaccgg agactgaatg gcctgttctc
     3781 ttttatcaag gaggagaacg atacacagaa gcacccaatc agccagctgc ggctgaggcg
     3841 cgagctggag atctaccagt ctctgagagt ggacgccttc aaggagacac tgagcctgga
     3901 ggagaagctg ctgaataagc acacatccct gtcctctctg gagaacgagt ttagggccct
     3961 gctggaggag tggaagaagg agtatgccgc cagctccatg gtgacagacg agcacatcgc
     4021 cttcatcgcc agcgtggcca atgccttctg cgccaaccag taccccttct acaaggaggc
     4081 cctgcacgcc cctatcccac tgttcaccgt ggcccagccc accacagagg agaaggatgg
     4141 cctgggaatc gcagaggccc tgctgaaggt gctgagggag tactgtgaga tcgtgaagtc
     4201 ccagatcgga tccagcctcc aaaaaaagtt ggaggaatta gaactaggat cccagctgca
     4261 tttaccgcag gttttagctg acgctgtctc acgcctggtc ataggtaagt ttggtgacct
     4321 gaccgacaac ttctcctccc ctcacgctcg cagaataggt ctggctggag tcgtcatgac
     4381 aacaggcaca gatgttaaag atgccaaggt gatatgtgtt tctacaggag caaaatgtat
     4441 taatggtgaa tacctaagtg atcgtggcct tgcattaaat gactgccatg cagaaatagt
     4501 atctcggaga tccttgctca gatttcttta tacacaactt gagctttact taaataacga
     4561 ggatgatcaa aaaagatcca tctttcagaa atcagagcga ggggggttta ggctgaagga
     4621 gaatatacag tttcatctgt acatcagcac ctctccctgt ggagatgcca gaatcttctc
     4681 accacatgag gcaatcctgg aagaaccagc agatagacac ccaaatcgta aagcaagagg
     4741 acagctacgg accaaaatag aggctggtca ggggacgatt ccagtgcgca acaatgcgag
     4801 catccaaacg tgggacgggg tgctgcaagg ggagcggctg ctcaccatgt cctgcagtga
     4861 caagattgca cgctggaacg tggtgggcat ccagggatca ctgctcagca ttttcgtgga
     4921 gcccatttac ttctcgagca tcatcctggg cagcctttac cacggggacc acctttccag
     4981 ggccatgtac cagcggatct ccaacataga ggacctgcca cctctctaca ccctcaacaa
     5041 gcctttgctc acaggcatca gcaatgcaga agcacggcag ccagggaagg cccccatatt
     5101 cagtgtcaac tggacggtag gcgactccgc tattgaggtc atcaacgcca cgactgggaa
     5161 gggagagctg ggccgcgcgt cccgcctgtg taagcacgcg ttgtactgtc gctggatgcg
     5221 tgtgcacggc aaggttccct cccacttact acgctccaag attaccaagc ccaacgtgta
     5281 ccatgagaca aagctggcgg caaaggagta ccaggccgcc aaggcgcgtc tgttcacagc
     5341 cttcatcaag gcggggctgg gggcctgggt ggagaagccc accgagcagg accagttctc
     5401 actcacgctt caactgcctc cacttgaaag actgacactg atggtgagca agggcgagga
     5461 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     5521 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     5581 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     5641 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     5701 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     5761 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     5821 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agacaatggg
     5881 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     5941 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     6001 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     6061 ctcccacaac gaggactaca caatcgtgga acagtacgaa cgcgccgagg gccgccactc
     6121 caccggcggc atggacgagc tgtacaagat cagtctgatt gcggcgttag cggtagatta
     6181 cgttatcggc atggaaaacg ccatgccgtg gaacctgcct gccgatctcg cctggtttaa
     6241 acgcaacacc ttaaataaac ccgtgattat gggccgccat acctgggaat caatcggtcg
     6301 tccgttgcca ggacgcaaaa atattatcct cagcagtcaa ccgagtacgg acgatcgcgt
     6361 aacgtgggtg aagtcggtgg atgaagccat cgcggcgtgt ggtgacgtac cagaaatcat
     6421 ggtgattggc ggcggtcgcg ttattgaaca gttcttgcca aaagcgcaaa aactgtatct
     6481 gacgcatatc gacgcagaag tggaaggcga cacccatttc ccggattacg agccggatga
     6541 ctgggaatcg gtattcagcg aattccacga tgctgatgcg cagaactctc acagctattg
     6601 ctttgagatt ctggagcggg cggccgctcg agtctagagg gcccgtttaa acccgctgat
     6661 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6721 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6781 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6841 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     6901 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     6961 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7021 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7081 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     7141 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     7201 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     7261 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     7321 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     7381 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7441 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7501 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7561 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7621 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     7681 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     7741 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     7801 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     7861 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     7921 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     7981 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     8041 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     8101 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     8161 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     8221 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     8281 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     8341 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     8401 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     8461 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     8521 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     8581 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     8641 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     8701 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     8761 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8821 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     8881 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     8941 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9001 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9061 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9121 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9181 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9241 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9301 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9361 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9421 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9481 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9541 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9601 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9661 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9721 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9781 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9841 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9901 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9961 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10021 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10081 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10141 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10201 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10261 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10321 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10381 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10441 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10501 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10561 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10621 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10681 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10741 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10801 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10861 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10921 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10981 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    11041 cgcacatttc cccgaaaagt gccacctgac gtc
//
