LOCUS       sequence-374365-        9693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the mCherry-Mango II x 24 array from a TetON responsive 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9693)
  AUTHORS   Cawte AD, Unrau PJ, Rueda DS
  TITLE     Live cell imaging of single RNA molecules with fluorogenic Mango II 
            arrays.
  JOURNAL   Nat Commun. 2020 Mar 9;11(1):1283. doi: 10.1038/s41467-020-14932-7.
  PUBMED    32152311
REFERENCE   2  (bases 1 to 9693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-03-9"; volume: "11(1):1283. doi"; pages: " 
            10.1038/s41467-020-14932-7"
COMMENT     Sequence Label: pLenti-mCherry-Mango II x 24
FEATURES             Location/Qualifiers
     source          1..9693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      499..1087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     988..1007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1241..1258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1375..1396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1411..1416,1417..1434,1435..1441)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1449..1465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1454..1476
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1473..1489
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1473..1489
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1508..1528
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1510..1528
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1556..1782
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1783..1963
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2010..2135
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2628..2861
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3046..3090
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3303..3420
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3474..3788
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3482..3500
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3518..3536
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3553..3571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3589..3607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3625..3643
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3660..3678
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3696..3714
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3761..3785
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3812..3821
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3818..4528
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3963..3981)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4231..4250
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5801..5882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5897..6407
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6326..6344
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6417..7013
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6417..6436)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6913..6933
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7014..7067
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7068..7685,7686..7691,7692..7727,7728..7730,7731..7766,
                     7767..7769,7770..7805,7806..7814)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7854..7874)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8272..8283)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8488..8721
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8793..8914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8954..9089
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9089..9160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9161..9229,9230..9693,1..328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9379..9398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
       61 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      121 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      181 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      241 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      301 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
      361 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      421 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
      481 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      541 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      601 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      661 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      721 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      781 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      841 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      901 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      961 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1021 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1081 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1141 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1201 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1261 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1321 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     1381 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     1441 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     1501 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     1561 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     1621 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     1681 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     1741 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     1801 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     1861 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     1921 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     1981 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     2041 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     2101 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     2161 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     2221 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     2281 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     2341 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     2401 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     2461 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     2521 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     2581 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     2641 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     2701 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     2761 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     2821 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     2881 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     2941 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     3001 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     3061 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     3121 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     3181 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     3241 cacctcccaa ccccgagggg acaattctcg acctcgagac aaatggcagt attcatccac
     3301 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     3361 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     3421 cgggtttatt acagggacag cagagatcca ctttggccgc gaatcgatat gtcgagttta
     3481 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga
     3541 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     3601 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat
     3661 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     3721 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     3781 agatcgcctg gagaattggc tagcaccggt cgccaccatg gtgagcaagg gcgaggagga
     3841 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     3901 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     3961 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     4021 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     4081 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     4141 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     4201 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     4261 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     4321 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     4381 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     4441 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     4501 cggcggcatg gacgagctgt acaagtaacg atcgtacggt acttattgcc aagaaaggca
     4561 cgtacgaagg agaggagagg aagaggagag tacgtgcttt ttggacatgc cggcacgtac
     4621 gaaggagagg agaggaagag gagaggcatg tcctttttgg gtgcatcgaa ggagaggaga
     4681 ggaagaggag agatgcaccc tttttggcac gtacgaagga gaggagagga agaggagagt
     4741 acgtgctttt tggacatgcc ggcacgtacg aaggagagga gaggaagagg agaggcatgt
     4801 cctttttggg tgcatcgaag gagaggagag gaagaggaga gatgcaccct ttttggcacg
     4861 tacgaaggag aggagaggaa gaggagagta cgtgcttttt ggacatgccg gcacgtacga
     4921 aggagaggag aggaagagga gaggcatgtc ctttttgggt gcatcgaagg agaggagagg
     4981 aagaggagag atgcaccctt tttggcacgt acgaaggaga ggagaggaag aggagagtac
     5041 gtgctttttg gacatgccgg cacgtacgaa ggagaggaga ggaagaggag aggcatgtcc
     5101 tttttgggtg catcgaagga gaggagagga agaggagaga tgcacccttt ttggcacgta
     5161 cgaaggagag gagaggaaga ggagagtacg tgctttttgg acatgccggc acgtacgaag
     5221 gagaggagag gaagaggaga ggcatgtcct ttttgggtgc atcgaaggag aggagaggaa
     5281 gaggagagat gcaccctttt tggcacgtac gaaggagagg agaggaagag gagagtacgt
     5341 gctttttgga catgccggca cgtacgaagg agaggagagg aagaggagag gcatgtcctt
     5401 tttgggtgca tcgaaggaga ggagaggaag aggagagatg cacccttttt ggcacgtacg
     5461 aaggagagga gaggaagagg agagtacgtg ctttttggac atgccggcac gtacgaagga
     5521 gaggagagga agaggagagg catgtccttt ttgggtgcat cgaaggagag gagaggaaga
     5581 ggagagatgc accctttttg gcacgtacga aggagaggag aggaagagga gagtacgtgc
     5641 tttttggaca tgccggcacg tacgaaggag aggagaggaa gaggagaggc atgtcctttt
     5701 tgggtgcatc gaaggagagg agaggaagag gagagatgca cccctccagt attccagggt
     5761 tcatcagatc cgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     5821 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     5881 ttaacaacaa ggatccgggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
     5941 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     6001 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     6061 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     6121 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     6181 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     6241 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     6301 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     6361 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagcaa ttcaccatga
     6421 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     6481 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     6541 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6601 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6661 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6721 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6781 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6841 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6901 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6961 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccgaaggta
     7021 gaggttctct cctcacttgt ggtgatgttg aagaaaaccc tggtccaatg tctagactgg
     7081 acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt atcgaaggcc
     7141 tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg
     7201 tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata
     7261 cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt
     7321 cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc
     7381 caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct
     7441 tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg
     7501 tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt
     7561 ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg
     7621 ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa
     7681 gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc
     7741 ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca
     7801 tgctccccgg gtaactaagt aaggatcgat ccaagatatc gtattcttaa ctatgttgct
     7861 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7921 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7981 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     8041 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8101 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8161 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     8221 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8281 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8341 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtttcgcct
     8401 cggcgtccgg actagaggta cctttaagac caatgactta caaggcagct gtagatctta
     8461 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     8521 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8581 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8641 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8701 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     8761 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     8821 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8881 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     8941 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9001 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9061 gaagtagtga ggaggctttt ttggaggcct ttcaaatatg tatccgctca tgagacaata
     9121 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     9181 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     9241 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     9301 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     9361 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     9421 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     9481 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     9541 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     9601 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9661 gaatgaagcc ataccaaacg acgagcgtga cac
//
