LOCUS       sequence-37437-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 4179 (=HL 865 + pHL 1381)
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(213..299)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    664..680
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(773..800)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
       61 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      121 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      181 cgcagccgaa cgccctaggt ctagggcggc ggatttgtcc tactcaggag agcgttcacc
      241 gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga
      301 tgcctctagc acgcgtacca tgggccctta ttcggtttct tcgctgtcct gttgcgcgga
      361 agtattctgc gcgctgctac catgatggta gttactgctg gtaccgccac cggcgttgtt
      421 actgtgatga gaaaccgggc gagacgggac aacagtagaa atcgcgtgct tgtaaaccat
      481 ctggctgacc gtgtttttca acaggatcac gaactgatca aaagactcga tttgcccttg
      541 cagcttaata ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt
      601 caggaacgga tcttgtaaag attgcccctt agccattttt tttcctcctt acccgggagt
      661 atcttgttat ccgctcacaa tgtcaatgtt atccgctcac atttatggat cctgagcgga
      721 tacatatttg aatgtattta gaaaaataaa caaaagagtt tgtagaaacg caaaaaggcc
      781 atccgtcagg atggccttct gcttaatttg atgcctggca g
//
