LOCUS       sequence-374372-        8518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the fusion protein containing the extracellular part of 
            the human Siglec-10 R47Q mutant and the Fc region of human IgG1, and
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Alborzian Deh Sheikh A, Gomaa S, Li X, Routledge M, Saigoh K, Numoto
            N, Angata T, Hitomi Y, Takematsu H, Tsuiji M, Ito N, Kusunoki S, 
            Tsubata T
  TITLE     A Guillain-Barre syndrome-associated SIGLEC10 rare variant impairs 
            its recognition of gangliosides.
  JOURNAL   J Autoimmun. 2021 Jan;116:102571. doi: 10.1016/j.jaut.2020.102571. 
            Epub 2020 Nov 19.
  PUBMED    33223341
REFERENCE   2  (bases 1 to 8518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Autoimmun."; date: "2021-01"; volume: "116:102571. doi"; pages: " 
            10.1016/j.jaut.2020.102571. Epub 2020 Nov 19"
COMMENT     Sequence Label: pMXs-Sig10(M1)-Fc-IG
FEATURES             Location/Qualifiers
     source          1..8518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    join(4546..5120,5121..5123,5124..5132)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4713..4730)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4940..4959
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5133..5135,5136..5138,5139..5852)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5133..5135,5136..5138,5139..5849)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5178..5199)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5439..5458)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5786..5807
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6023..6492
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6622..6639)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6793..7381)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6873..6892)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7545..8336,8337..8405))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8168..8187
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8406..8510)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attcgagctc ggtacctcgc gaatgcatct agagccacca
     1981 tgctactgcc actgctgctg tcctcgctgc tgggcgggtc ccaggctatg gatgggagat
     2041 tctggatacg agtgcaggag tcagtgatgg tgccggaggg cctgtgcatc tctgtgccct
     2101 gctctttctc ctacccccaa caggactgga cagggtctac cccagcttat ggctactggt
     2161 tcaaagcagt gactgagaca accaagggtg ctcctgtggc cacaaaccac cagagtcgag
     2221 aggtggaaat gagcacccgg ggccgattcc agctcactgg ggatcccgcc aaggggaact
     2281 gctccttggt gatcagagac gcgcagatgc aggatgagtc acagtacttc tttcgggtgg
     2341 agagaggaag ctatgtgaga tataatttca tgaacgatgg gttctttcta aaagtaacag
     2401 ccctgactca gaagcctgat gtctacatcc ccgagaccct ggagcccggg cagccggtga
     2461 cggtcatctg tgtgtttaac tgggcctttg aggaatgtcc acccccttct ttctcctgga
     2521 cgggggctgc cctctcctcc caaggaacca aaccaacgac ctcccacttc tcagtgctca
     2581 gcttcacgcc cagaccccag gaccacaaca ccgacctcac ctgccatgtg gacttctcca
     2641 gaaagggtgt gagcgcacag aggaccgtcc gactccgtgt ggcctatgcc cccagagacc
     2701 ttgttatcag catttcacgt gacaacacgc cagccctgga gccccagccc cagggaaatg
     2761 tcccatacct ggaagcccaa aaaggccagt tcctgcggct cctctgtgct gctgacagcc
     2821 agccccctgc cacactgagc tgggtcctgc agaacagagt cctctcctcg tcccatccct
     2881 ggggccctag acccctgggg ctggagctgc ccggggtgaa ggctggggat tcagggcgct
     2941 acacctgccg agcggagaac aggcttggct cccagcagcg agccctggac ctctctgtgc
     3001 agtatcctcc agagaacctg agagtgatgg tttcccaagc aaacaggaca gtcctggaaa
     3061 accttgggaa cggcacgtct ctcccagtac tggagggcca aagcctgtgc ctggtctgtg
     3121 tcacacacag cagcccccca gccaggctga gctggaccca gaggggacag gttctgagcc
     3181 cctcccagcc ctcagacccc ggggtcctgg agctgcctcg ggttcaagtg gagcacgaag
     3241 gagagttcac ctgccacgct cggcacccac tgggctccca gcacgtctct ctcagcctct
     3301 ccgtgcacta ctccccgaag ctgctgggcc cctcctgctc ctgggaggct gagggtctgc
     3361 actgcagctg ctcctcccag gccagcccgg ccccctctct gcgctggtgg cttggggagg
     3421 agctgctgga ggggaacagc agccaggact ccttcgaggt cacccccagc tcagccgggc
     3481 cctgggccaa cagctccctg agcctccatg gagggctcag ctccggcctc aggctccgct
     3541 gtgaggcctg gaacgtccat ggggcccaga gtggatccat cctgcagctg ccagataagc
     3601 tcgagcccaa atcttgtgac aaaactcaca catgcccacc gtgcccaggt aagccagccc
     3661 aggcctcgcc ctccagctca aggcgggaca ggtgccctag agtagcctgc atccagggac
     3721 aggccccagc cgggtgctga cacgtccacc tccatctctt cctcagcacc tgaactcctg
     3781 gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat gatctcccgg
     3841 acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc
     3901 aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag
     3961 tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat
     4021 ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat cgagaaaacc
     4081 atctccaaag ccaaaggtgg gacccgtggg gtgcgagggc cacatggaca gaggccggct
     4141 cggcccaccc tctgccctgg gagtgaccgc tgtaccaacc tctgtcccta cagggcagcc
     4201 ccgagaacca caggtgtaca ccctgccccc atcccgggat gagctgacca agaaccaggt
     4261 cagcctgacc tgcctggtca aaggcttcta tcccagcgac atcgccgtgg agtgggagag
     4321 caatgggcag ccggagaaca actacaaggc cacgcctccc gtgctggact ccgacggctc
     4381 cttcttcctc tacagcaagc tcaccgtgga caagagcagg tggcagcagg ggaacgtctt
     4441 ctcatgctcc gtgatgcatg aggctctgca caaccactac acgcagaaga gcctctccct
     4501 gtccccgggt aaatagcggc cgctacgtaa attccscccc ccccccccnt ctccctcccc
     4561 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     4621 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     4681 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     4741 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     4801 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     4861 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     4921 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     4981 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     5041 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     5101 ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag gagctgttca
     5161 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     5221 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     5281 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     5341 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     5401 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     5461 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     5521 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     5581 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     5641 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     5701 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     5761 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     5821 tcactctcgg catggacgag ctgtacaagt aagtcgacga taaaataaaa gattttattt
     5881 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     5941 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
     6001 aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     6061 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
     6121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     6181 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     6241 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     6301 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
     6361 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     6421 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     6481 gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta
     6541 cattaaatgg ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6601 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6661 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6721 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6781 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6841 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6901 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6961 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7021 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7081 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7141 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7201 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7261 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7321 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7381 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7441 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccggc
     7501 cgcaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7561 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7621 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7681 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7741 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7801 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7861 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7921 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7981 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8041 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8101 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8161 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8221 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8281 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8341 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8401 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8461 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttc
//
