LOCUS       sequence-374390-       12180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression ADAM10 in mammalian cells. Contains only 
            prodomain through cysteine rich domain--amino acids 1-654.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12180)
  TITLE     TREAT-AD plasmids 2022
REFERENCE   2  (bases 1 to 12180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSCM167.2-Venus-VF-hADAM10(aa1-654)
FEATURES             Location/Qualifiers
     source          1..12180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     1297..1316
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1297..1315
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1332..1334,1335..1337,1338..2048)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1377..1398)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1638..1657)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1985..2006
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2088..2111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2133..2157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4141..4165)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(4184..4202)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4185..4202)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    4251..4839
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4304..4324)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4722..4733)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4842..4858)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4843..4859)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5364..5544
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5570..5587)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5576..5800
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5846..6274
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5933..5952)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6143..6164
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6283..6303)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6288..6617
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6468..6603
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6530..6549
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6665..6712
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6731..7105
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7235..7356
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7272..7291)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7326..7345
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7405..7421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7405..7421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7418..7440)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7429..7445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7453..7459,7460..7477,7478..7483))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7498..7519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7636..7653)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7807..8395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7887..7906)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8566..9357,9358..9426))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9189..9208
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9427..9531)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9606..9625)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9797..10176
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10178..10376
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10331..10351
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10394..10574
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10621..10746
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11239..11472
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11657..11701
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11999..12116
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactctaga ggatccagtt ctctctggct
     1261 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
     1321 ccaagcttac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1381 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1441 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
     1501 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
     1561 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1621 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1681 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1741 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     1801 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     1861 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1921 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1981 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2041 tgtacaaggg agggggtgga tctggagggg gtggatctaa gcttatggac tacaaggacg
     2101 acgatgacaa gggtggtgga tccgaattat caacaagttt gtacaaaaaa gcaggcttcg
     2161 aaggagatag aaccatggcc gtgttgctga gagtgttaat tctgctcctc tcctgggcgg
     2221 cggggatggg aggtcagtat gggaatcctt taaataaata tatcagacat tatgaaggat
     2281 tatcttacaa tgtggattca ttacaccaaa aacaccagcg tgccaaaaga gcagtctcac
     2341 atgaagacca attttcacgt ctagatttcc atgcccatgg aagacatttc aacctacgaa
     2401 tgaagaggga cacttccctt ttcagtgatg aatttaaagt agaaacatca aataaagtac
     2461 ttggttatga tacctctcat atttacactg gacatattta tggtgaagaa ggaagttttg
     2521 gccatgggtc tgttattgat ggaagatttg aaggattcat ccagactcgt ggtggcacat
     2581 tttatgttga gccagcagag agatatatta aagaccgaac tctgccattt cactctgtca
     2641 tttatcatga agatgatatt aactatcccc ataaatacgg tcctcagggg ggctgtgcag
     2701 atcattcagt atttgaaaga atgaggaaat accagatgac tggtgtagag gaagtaacac
     2761 agatacctca agaagaacat gctgctaatg gtccagaact tctgaggaaa aaacgtacaa
     2821 cttcagctga aaaaaatact tgtcagcttt atattcagac tgatcatttg ttctttaaat
     2881 attacggaac acgagaagct gtgattgccc agatatccag tcatgttaaa gcgattgata
     2941 caatttacca gaccacagac ttctccggaa tccgtaacat cagtttcatg gtgaaacgca
     3001 taagaatcaa tacaactgct gatgagaagg accctacgaa tcctttccgt ttcccaaata
     3061 ttggtgtgga gaagtttctg gaattgaatt ctgagcagaa tcatgatgac tactgtttgg
     3121 cccatgtctt cacagaccga gattttgatg atggcgtact tggtctggct tgggttggag
     3181 caccttcagg aagctctgga ggaatatgtg aaaaaagtaa actctattca gatggtaaga
     3241 agaagtcctt aaacactgga attattactg ttcagaacta tgggtctcat gtacctccca
     3301 aagtctctca cattactttt gctcacgaag ttggacataa ctttggatcc ccacatgatt
     3361 ctggaacaga gtgcacacca ggagaatcta agaatttggg tcaaaaagaa aatggcaatt
     3421 acatcatgta tgcaagagca acatctgggg acaaacttaa caacaataaa ttctcactct
     3481 gtagtattag aaatataagc caagttcttg agaagaagag aaacaactgt tttgttgaat
     3541 ctggccaacc tatttgtgga aatggaatgg tagaacaagg tgaagaatgt gattgtggct
     3601 atagtgacca gtgtaaagat gaatgctgct tcgatgcaaa tcaaccagag ggaagaaaat
     3661 gcaaactgaa acctgggaaa cagtgcagtc caagtcaagg tccttgttgt acagcacagt
     3721 gtgcattcaa gtcaaagtct gagaagtgtc gggatgattc agactgtgca agggaaggaa
     3781 tatgtaatgg cttcacagct ctctgcccag catctgaccc taaaccaaac ttcacagact
     3841 gtaataggca tacacaagtg tgcattaatg ggcaatgtgc aggttctatc tgtgagaaat
     3901 atggcttaga ggagtgtacg tgtgccagtt ctgatggcaa agatgataaa gaattatgcc
     3961 atgtatgctg tatgaagaaa atggacccat caacttgtgc cagtacaggg tctgtgcagt
     4021 ggagtaggca cttcagtggt cgaaccatca ccctgcaacc tggatcccct tgcaacgatt
     4081 ttagaggtta ctgtgatgtc ttcatgcggt gcagattagt agatgctgat ggtcctctaa
     4141 acccagcttt cttgtacaaa gtggttgatc tagagggccc tattctatag tgtcacctaa
     4201 atgctagaga acctcgaggg cgcgccgaat tcgatatcaa gcttatcgat aatcaacctc
     4261 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4321 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4381 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4441 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4501 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4561 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4621 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     4681 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4741 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4801 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     4861 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     4921 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     4981 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5041 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     5101 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     5161 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     5221 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     5281 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     5341 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5401 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5461 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5521 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     5581 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5641 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5701 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5761 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     5821 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5881 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5941 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6001 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6061 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6121 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6181 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6241 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     6301 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6361 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6421 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6481 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6541 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6601 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     6661 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     6721 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     6781 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     6841 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     6901 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     6961 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     7021 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     7081 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     7141 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7201 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7261 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7321 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7381 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7441 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7501 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7561 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7621 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7681 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7741 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7801 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7861 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7921 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7981 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8041 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8101 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8161 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8221 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8281 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8341 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8401 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8461 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8521 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8581 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8641 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8701 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8761 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8821 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8881 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8941 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9001 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9061 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9121 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9181 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9241 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9301 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9361 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9421 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9481 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9541 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     9601 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     9661 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     9721 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     9781 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     9841 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     9901 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     9961 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
    10021 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
    10081 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
    10141 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
    10201 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
    10261 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
    10321 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
    10381 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    10441 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    10501 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    10561 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
    10621 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    10681 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    10741 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    10801 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    10861 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    10921 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    10981 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    11041 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
    11101 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
    11161 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    11221 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    11281 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    11341 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    11401 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    11461 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    11521 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    11581 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    11641 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    11701 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    11761 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
    11821 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
    11881 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
    11941 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
    12001 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
    12061 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
    12121 tttattacag ggacagcaga gatccagttt ggttaattaa cccgtgtcgg ctccagatct
//
