LOCUS       sequence-374394-       10533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression ADAM10 in mammalian cells. Contains only cysteine
            rich domain--amino acids 551-654.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10533)
  TITLE     TREAT-AD plasmids 2022
REFERENCE   2  (bases 1 to 10533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSCM167.2-Venus-VF-hADAM10(aa551-654)
FEATURES             Location/Qualifiers
     source          1..10533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     1297..1316
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1297..1315
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1332..1334,1335..1337,1338..2048)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1377..1398)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1638..1657)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1985..2006
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2088..2111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2133..2157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2494..2518)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2537..2555)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2538..2555)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    2604..3192
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2657..2677)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3075..3086)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3195..3211)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3196..3212)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3717..3897
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3923..3940)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3929..4153
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4199..4627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4286..4305)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4496..4517
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4636..4656)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4641..4970
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4821..4956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4883..4902
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5018..5065
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5084..5458
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5588..5709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5625..5644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5679..5698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5758..5774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5758..5774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5771..5793)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5782..5798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5806..5812,5813..5830,5831..5836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5851..5872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5989..6006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6160..6748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6240..6259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6919..7710,7711..7779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7542..7561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7780..7884)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7959..7978)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8150..8529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8531..8729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8684..8704
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8747..8927
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8974..9099
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9592..9825
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10010..10054
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10352..10469
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactctaga ggatccagtt ctctctggct
     1261 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
     1321 ccaagcttac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1381 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1441 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
     1501 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
     1561 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1621 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1681 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1741 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     1801 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     1861 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1921 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1981 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2041 tgtacaaggg agggggtgga tctggagggg gtggatctaa gcttatggac tacaaggacg
     2101 acgatgacaa gggtggtgga tccgaattat caacaagttt gtacaaaaaa gcaggcttcg
     2161 aaggagatag aaccatggcc aacttcacag actgtaatag gcatacacaa gtgtgcatta
     2221 atgggcaatg tgcaggttct atctgtgaga aatatggctt agaggagtgt acgtgtgcca
     2281 gttctgatgg caaagatgat aaagaattat gccatgtatg ctgtatgaag aaaatggacc
     2341 catcaacttg tgccagtaca gggtctgtgc agtggagtag gcacttcagt ggtcgaacca
     2401 tcaccctgca acctggatcc ccttgcaacg attttagagg ttactgtgat gtcttcatgc
     2461 ggtgcagatt agtagatgct gatggtcctc taaacccagc tttcttgtac aaagtggttg
     2521 atctagaggg ccctattcta tagtgtcacc taaatgctag agaacctcga gggcgcgccg
     2581 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     2641 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     2701 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     2761 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2821 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     2881 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     2941 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     3001 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3061 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3121 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3181 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     3241 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     3301 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     3361 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     3421 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     3481 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     3541 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     3601 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     3661 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     3721 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     3781 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     3841 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     3901 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3961 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4021 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4081 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4141 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4201 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4261 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4321 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4381 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4441 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4501 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4561 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4621 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     4681 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4741 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4801 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4861 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4921 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4981 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     5041 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     5101 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     5161 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     5221 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     5281 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     5341 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     5401 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     5461 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     5521 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     5581 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5641 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5701 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     5761 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5821 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5881 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5941 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6001 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6061 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6541 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6601 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6841 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6901 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6961 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7021 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7081 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7141 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7201 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7261 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7321 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7381 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7441 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7501 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7561 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7621 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7681 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7741 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7861 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     7921 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     7981 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     8041 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     8101 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     8161 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     8221 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     8281 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     8341 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     8401 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     8461 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     8521 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     8581 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     8641 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     8701 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     8761 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8821 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8881 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     8941 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     9001 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     9061 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     9121 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     9181 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     9241 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     9301 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     9361 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     9421 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     9481 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     9541 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     9601 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     9661 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     9721 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     9781 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     9841 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     9901 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9961 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    10021 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    10081 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    10141 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    10201 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    10261 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
    10321 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    10381 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    10441 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
    10501 tttggttaat taacccgtgt cggctccaga tct
//
