LOCUS       sequence-374395-       11139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression of SFRP1 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11139)
  TITLE     TREAT-AD plasmids 2022
REFERENCE   2  (bases 1 to 11139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSCM167.2-Venus-VF-hSFRP1
FEATURES             Location/Qualifiers
     source          1..11139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     1297..1316
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1297..1315
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1332..1334,1335..1337,1338..2048)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1377..1398)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1638..1657)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1985..2006
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2088..2111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2133..2157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3100..3124)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3143..3161)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3144..3161)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    3210..3798
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3263..3283)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3681..3692)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3801..3817)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3802..3818)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4323..4503
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4529..4546)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4535..4759
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4805..5233
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4892..4911)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5102..5123
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5242..5262)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5247..5576
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5427..5562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5489..5508
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5624..5671
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5690..6064
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6194..6315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6231..6250)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6285..6304
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6364..6380)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6364..6380)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6377..6399)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6388..6404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6412..6418,6419..6436,6437..6442))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6457..6478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6595..6612)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6766..7354)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6846..6865)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7525..8316,8317..8385))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8148..8167
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8386..8490)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8565..8584)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8756..9135
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9137..9335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9290..9310
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9353..9533
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9580..9705
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10198..10431
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10616..10660
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10958..11075
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactctaga ggatccagtt ctctctggct
     1261 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
     1321 ccaagcttac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1381 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1441 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
     1501 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
     1561 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1621 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1681 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1741 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     1801 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     1861 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1921 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1981 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2041 tgtacaaggg agggggtgga tctggagggg gtggatctaa gcttatggac tacaaggacg
     2101 acgatgacaa gggtggtgga tccgaattat caacaagttt gtacaaaaaa gttggcatgg
     2161 gcatcgggcg cagcgagggg ggccgccgcg gggcagccct gggcgtgctg ctggcgctgg
     2221 gcgcggcgct tctggccgtg ggctcggcca gcgagtacga ctacgtgagc ttccagtcgg
     2281 acatcggccc gtaccagagc gggcgcttct acaccaagcc acctcagtgc gtggacatcc
     2341 ccgcggacct gcggctgtgc cacaacgtgg gctacaagaa gatggtgctg cccaacctgc
     2401 tggagcacga gaccatggcg gaggtgaagc agcaggccag cagctgggtg cccctgctca
     2461 acaagaactg ccacgccggc acccaggtct tcctctgctc gctcttcgcg cccgtctgcc
     2521 tggaccggcc catctacccg tgtcgctggc tctgcgaggc cgtgcgcgac tcgtgcgagc
     2581 cggtcatgca gttcttcggc ttctactggc ccgagatgct taagtgtgac aagttccccg
     2641 agggggacgt ctgcatcgcc atgacgccgc ccaatgccac cgaagcctcc aagccccaag
     2701 gcacaacggt gtgtcctccc tgtgacaacg agttgaaatc tgaggccatc attgaacatc
     2761 tctgtgccag cgagtttgca ctgaggatga aaataaaaga agtgaaaaaa gaaaatggcg
     2821 acaagaagat tgtccccaag aagaagaagc ccctgaagtt ggggcccatc aagaagaagg
     2881 acctgaagaa gcttgtgctg tacctgaaga atggggctga ctgtccctgc caccagctgg
     2941 acaacctcag ccaccacttc ctcatcatgg gccgcaaggt gaagagccag tacttgctga
     3001 cggccatcca caagtgggac aagaaaaaca aggagttcaa aaacttcatg aagaaaatga
     3061 aaaaccatga gtgccccacc tttcagtccg tgtttaagtg cccaactttc ttgtacaaag
     3121 tggttgatct agagggccct attctatagt gtcacctaaa tgctagagaa cctcgagggc
     3181 gcgccgaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     3241 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3301 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3361 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3421 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3481 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3541 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3601 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3661 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3721 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3781 tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa
     3841 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     3901 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     3961 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4021 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     4081 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct
     4141 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc
     4201 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt
     4261 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta
     4321 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4381 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4441 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4501 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     4561 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     4621 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     4681 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     4741 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     4801 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4861 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4921 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4981 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5041 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5101 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5161 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5221 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     5281 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     5341 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5401 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     5461 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     5521 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     5581 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     5641 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     5701 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     5761 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     5821 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     5881 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     5941 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     6001 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     6061 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6121 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     6181 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6241 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6301 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     6361 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6421 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6481 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6541 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6601 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6661 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6721 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6781 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6841 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6901 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6961 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7021 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7081 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7141 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7201 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7261 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7321 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7381 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7441 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7501 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7561 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7621 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7681 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7741 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7801 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7861 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7921 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7981 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8041 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8101 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8161 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8221 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8281 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8341 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8401 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8461 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8521 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     8581 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     8641 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     8701 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     8761 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     8821 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     8881 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     8941 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     9001 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     9061 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     9121 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     9181 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     9241 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     9301 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
     9361 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9421 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9481 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     9541 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     9601 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     9661 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     9721 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     9781 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     9841 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     9901 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     9961 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    10021 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
    10081 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
    10141 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
    10201 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
    10261 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
    10321 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
    10381 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
    10441 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
    10501 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
    10561 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
    10621 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    10681 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
    10741 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
    10801 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
    10861 aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc
    10921 gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
    10981 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
    11041 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
    11101 atccagtttg gttaattaac ccgtgtcggc tccagatct
//
