LOCUS       sequence-374406-        4386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF350 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF350
FEATURES             Location/Qualifiers
     source          1..4386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(33..60)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(152..238)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     287..309
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     301..318
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     302..318
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    334..433
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2030..2129)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2146..2165)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2147..2165)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2170..2186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2170..2186)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2617..3408
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3501..4089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3990..4009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4243..4260
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
       61 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
      121 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
      181 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
      241 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
      301 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata
      361 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac
      421 aaaaaagttg gcatgatcca ggcccaggaa tccataacac tggaggatgt ggctgtggac
      481 ttcacttggg aggagtggca actcctgggc gctgctcaga aggacctgta ccgggacgtg
      541 atgttggaga actacagcaa cctggtggca gtggggtatc aagccagcaa accggatgca
      601 ctcttcaagt tggaacaagg agaacaactg tggacaattg aagatggaat ccacagtgga
      661 gcctgttcag acatatggaa agttgatcat gtgctggagc gcttgcagag tgaaagcctg
      721 gtgaacagaa ggaaaccatg tcatgaacat gatgcatttg aaaatattgt tcattgcagc
      781 aaaagtcagt ttctgttagg gcaaaatcat gatatatttg acttacgtgg aaaaagtttg
      841 aaatccaatt taactttagt taaccagagc aaaggctatg aaataaagaa ctctgttgag
      901 tttactggaa atggggactc ctttcttcat gctaaccatg aacgacttca tactgcaatt
      961 aaattccctg caagtcaaaa actcatcagc actaagtccc aattcatcag tcccaagcat
     1021 cagaaaacac gaaaattaga gaagcatcat gtgtgcagtg aatgtgggaa agccttcatc
     1081 aagaagtctt ggctaactga tcaccaggta atgcatacag gagagaaacc ccacagatgt
     1141 agtctatgtg agaaagcctt ctccagaaag ttcatgctta ctgaacatca gcgaactcat
     1201 acaggagaaa aaccttatga atgccctgaa tgtggcaaag cctttctcaa gaaatcacgg
     1261 ctcaacatac atcagaaaac acataccgga gagaaaccct atatatgcag tgaatgtgga
     1321 aaaggcttca tccagaaagg aaatctcatt gtacaccagc gaattcatac aggtgagaaa
     1381 ccttatatat gcaatgaatg tggaaaaggc ttcattcaga agacgtgtct catagcacat
     1441 cagagatttc acacaggaaa gacgcccttt gtgtgcagtg aatgtggaaa atcctgttct
     1501 cagaaatcag gtctcattaa acatcaaaga attcacacag gagagaaacc ctttgaatgt
     1561 agtgaatgtg ggaaagcctt tagcacaaag caaaagctca ttgtccatca aaggactcat
     1621 acaggagaga gaccctatgg ctgtaacgag tgtgggaaag cgtttgcgta tatgtcgtgt
     1681 ctggttaagc ataagagaat acacacaagg gagaaacaag aggcagccaa ggtggaaaat
     1741 cctcctgcag agaggcacag ctcattacac accagtgatg tcatgcagga gaaaaactct
     1801 gctaacgggg cgactacaca agtgccttct gtggcccctc agacatcatt aaacatcagc
     1861 ggcctcctcg caaacaggaa cgtagtcctt gtgggacagc cagtggtcag atgtgcagcc
     1921 tcaggagata acagaggatt tgcacaggac agaaaccttg tgaatgcagt gaatgtggtt
     1981 gtgccttccg tgatcaatta tgtcttattt tatgttacag aaaacccata cccaactttc
     2041 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     2101 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     2161 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2221 tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat
     2281 gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     2341 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     2401 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     2461 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     2521 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     2581 cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa
     2641 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     2701 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     2761 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     2821 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     2881 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     2941 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     3001 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     3061 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     3121 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     3181 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     3241 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     3301 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     3361 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac
     3421 ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3481 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3541 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3601 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3661 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3721 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3781 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3841 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3901 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3961 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4021 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4081 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4141 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4201 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4261 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4321 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4381 acgcgt
//
