LOCUS       sequence-374438-        5599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the protein expression of Vps34 in a bacterial and 
            insect expression system. Internal_Reference: SMC1323.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5599)
  TITLE     ASAP - PI3KC3-C1 related projects
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGB-06-07-Vps34
FEATURES             Location/Qualifiers
     source          1..5599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  6..229
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(296..829)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1018..1023,1024..1040,1041..1046))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(1117..1150)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1239..1330
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1255..1272
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     RBS             2771..2779
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4100..4117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4261..4395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4298..4317)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4352..4371
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4527..4692
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4882..5470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5371..5390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat
       61 aaagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa
      121 agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc
      181 ccgtcgtatt aaagaggggc gtggccaagg gcatgtaaag actatattcg cggcgttgtg
      241 acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg
      301 tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat
      361 agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa
      421 gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt
      481 gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg
      541 gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga
      601 tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt
      661 aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga
      721 cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg
      781 ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc
      841 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
      901 gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
      961 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt
     1021 ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg
     1081 cgtcacccgg caaccttggg cagcagcgaa gtcgccataa cttcgtatag catacattat
     1141 acgaagttat ctgtaactat aacggtccta aggtagcgaa ggtctcaact agtttaaaca
     1201 ctagtatcga ttcgcgacct actccggaat attaatagat catggagata attaaaatga
     1261 taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa
     1321 acctataaat attccggatt attcataccg tcccaccatc gggcgacgga tcccggtccg
     1381 aaaccatggg cgaagcggag aaattccact acatctactc ttgcgacttg gacatcaacg
     1441 ttcaactcaa aatcggttca ctcgaaggta aacgcgagca gaagtcctac aaggccgtgc
     1501 tggaagaccc tatgctgaag ttctcaggtc tgtaccagga aacctgctcc gacctgtacg
     1561 tgacttgcca ggtcttcgct gagggcaagc ctctggctct gcccgtgcgc accagctaca
     1621 aggctttctc tactcgttgg aactggaacg agtggctgaa gctgcccgtc aagtaccccg
     1681 acctgccacg taacgctcag gtggccctga ccatctggga cgtgtacggt cccggcaagg
     1741 ccgtgccagt cggtggcacc actgtgtcac tgttcggcaa gtacggaatg ttccgccagg
     1801 gaatgcacga cctgaaagtg tggccaaacg tcgaggctga cggctccgaa ccaaccaaga
     1861 ctcctggacg tacctccagc actctgtcag aggaccagat gtccaggctg gctaagctga
     1921 ccaaggccca cagacagggt cacatggtga aggtggactg gctggaccgc ctgactttcc
     1981 gtgagatcga aatgatcaac gagagcgaaa agcgctcttc aaacttcatg tacctgatgg
     2041 tggagttccg ttgcgtcaag tgcgacgaca aggaatacgg tatcgtgtac tacgagaagg
     2101 acggcgacga atccagccct atcctgacct ctttcgagct ggtgaaggtc ccagaccctc
     2161 agatgagcat ggagaacctg gtcgaatcta agcaccacaa gctggctcgc tccctgcgta
     2221 gcggtccatc tgaccacgac ctgaagccaa acgctgccac tagggaccag ctgaacatca
     2281 tcgtgagcta ccctcccacc aagcagctga cttacgagga acaggacctg gtctggaagt
     2341 tcagatacta cctgaccaac caggagaagg ctctgactaa gttcctgaag tgcgtgaact
     2401 gggacctgcc acaggaggct aagcaggccc tggaactgct gggcaagtgg aagccaatgg
     2461 acgtcgagga ctctctggaa ctgctgtctt cacactacac caacccaact gtgcgccgtt
     2521 acgctgtcgc caggctgaga caggccgacg acgaagacct gctgatgtac ctgctgcaac
     2581 tggtgcaggc tctgaagtac gagaacttcg acgacatcaa gaacggactg gaacctacca
     2641 agaaggacag ccagtccagc gtgtctgaga acgtctctaa ctcaggtatc aactcagccg
     2701 aaatcgactc ttcacagatc atcacctcac ccctgccatc cgtgtccagc ccacctcccg
     2761 cttccaagac taaggaggtc cccgacggcg agaacctgga acaggacctg tgcaccttcc
     2821 tgatcagccg cgcttgcaag aactctactc tggccaacta cctgtactgg tacgtgatcg
     2881 tcgagtgcga agaccaggac acccagcagc gtgacccaaa gactcacgaa atgtacctga
     2941 acgtgatgag gagattctcc caggctctgc tgaagggaga caagtcagtg agggtcatga
     3001 gatccctgct ggctgcccag cagaccttcg tggaccgcct ggtccacctg atgaaggccg
     3061 tccagaggga gagcggaaac cgcaagaaga agaacgaacg tctgcaagct ctgctgggtg
     3121 acaacgagaa gatgaacctg tctgacgtgg agctgatccc tctgcccctg gaacctcagg
     3181 tcaagatcag gggtatcatc cctgaaaccg ctactctgtt caagtcagct ctgatgccag
     3241 cccagctgtt cttcaagact gaagacggtg gcaagtaccc tgtgatcttc aagcacggcg
     3301 acgacctgag gcaggaccag ctgatcctgc aaatcatctc cctgatggac aagctgctga
     3361 gaaaggagaa cctggacctg aagctgaccc cttacaaggt gctggccacc agcactaagc
     3421 acggcttcat gcagttcatc cagtctgtgc ccgtcgctga ggtcctggac actgaaggaa
     3481 gcatccagaa cttcttccgt aagtacgctc ctagcgagaa cggtcccaac ggcatctctg
     3541 ccgaagtgat ggacacctac gtcaagtctt gcgctggcta ctgcgtgatc acttacatcc
     3601 tgggagtcgg tgacaggcac ctggacaacc tgctgctgac caagactgga aagctgttcc
     3661 acatcgactt cggatacatc ctgggtcgcg accctaagcc tctgccacct cccatgaagc
     3721 tgaacaagga gatggtggag ggtatgggcg gaacccagtc agagcagtac caggagttca
     3781 ggaagcagtg ctacactgcc ttcctgcacc tgcgccgtta ctcaaacctg atcctgaacc
     3841 tgttctccct gatggtggac gctaacatcc cagacatcgc cctggagcct gacaagaccg
     3901 tgaagaaggt gcaagacaag ttcagactgg acctgtccga cgaggaagct gtgcactaca
     3961 tgcagtcact gatcgacgaa tccgtccacg cactcttcgc agcggtcgtt gagcagattc
     4021 ataagttcgc acaatactgg agaaaataac gccattaacc tgatgttctg gggaatataa
     4081 gcttgcggcc gcactcgagc accaccacca ccaccactga catgcggtac caagcttgtc
     4141 gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     4201 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     4261 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4321 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4381 tatcatgtct ggatctgatc actgcttgag cctagaagat ccggctgcta acaaagcccg
     4441 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctatcataac ccctagggta
     4501 tacccatcta attggcctgt gagaccaacc agataagtga aatctagttc caaactattt
     4561 tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc
     4621 actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt
     4681 tgtccgccca caaccggttg acttgggtca actgtcagac caagtttact catatatact
     4741 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4801 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4861 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4921 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4981 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5041 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5101 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5161 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5221 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5281 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5341 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5401 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5461 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5521 tgctcacatg ttctttcctg cgttatcccc tgattgactt gggtcgctct tcctgtggat
     5581 gcgcagatgc cctgcgtaa
//
