LOCUS       sequence-374440-        5098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the protein expression of GST-ATG14 in a bacterial and 
            insect expression system. Internal_Reference: SMC1321.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5098)
  TITLE     ASAP - PI3KC3-C1 related projects
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGB-05-06-GST-ATG14
FEATURES             Location/Qualifiers
     source          1..5098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  22..245
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(312..845)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1034..1039,1040..1056,1057..1062))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(1133..1166)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1255..1346
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1271..1288
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1402..2055
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     2013..2035
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             2062..2085
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3615..3632
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3776..3910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3813..3832)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3867..3886
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4042..4207
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4397..4985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4886..4905
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
       61 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      121 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      181 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ccaagggcat gtaaagacta
      241 tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct
      301 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt
      361 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag
      421 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca
      481 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc
      541 ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg
      601 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc
      661 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca
      721 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt
      781 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt
      841 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
      901 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac
      961 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
     1021 ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat
     1081 ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg ccataacttc
     1141 gtatagcata cattatacga agttatctgt aactataacg gtcctaaggt agcgaaggtc
     1201 tcactgcgtt taaacactag tatcgattcg cgacctactc cggaatatta atagatcatg
     1261 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
     1321 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
     1381 gacggatccc ggtccgaaac catgtcccct atactaggtt attggaaaat taagggcctt
     1441 gtgcaaccca ctcgacttct tttggaatat cttgaagaaa aatatgaaga gcatttgtat
     1501 gagcgcgatg aaggtgataa atggcgaaac aaaaagtttg aattgggttt ggagtttccc
     1561 aatcttcctt attatattga tggtgatgtt aaattaacac agtctatggc catcatacgt
     1621 tatatagctg acaagcacaa catgttgggt ggttgtccaa aagagcgtgc agagatttca
     1681 atgcttgaag gagcggtttt ggatattaga tacggtgttt cgagaattgc atatagtaaa
     1741 gactttgaaa ctctcaaagt tgattttctt agcaagctac ctgaaatgct gaaaatgttc
     1801 gaagatcgtt tatgtcataa aacatattta aatggtgatc atgtaaccca tcctgacttc
     1861 atgttgtatg acgctcttga tgttgtttta tacatggacc caatgtgcct ggatgcgttc
     1921 ccaaaattag tttgttttaa aaaacgtatt gaagctatcc cacaaattga taagtacttg
     1981 aaatccagca agtatatagc atggcctttg cagggctggc aagccacgtt tggtggtggc
     2041 gaccatcctc caaaatcgga tctggaagtt ctgttccagg ggcccatggc atcgccaagc
     2101 ggaaaaggag caagagccct ggaagcaccc ggctgcggac caagaccact cgcccgtgac
     2161 ctcgttgata gcgtggacga cgctgagggt ctgtacgtgg ccgtcgaacg ttgccctctg
     2221 tgcaacacca ctcgccgtag gctgacctgc gctaagtgcg tgcagagcgg tgacttcgtc
     2281 tacttcgacg gacgcgaccg cgagcgtttc atcgacaaga aggaaaggct gtccagactg
     2341 aagagcaagc aggaggagtt ccagaaggag gtcctgaagg ctatggaggg caagtggatc
     2401 actgaccagc tgcgctggaa gatcatgtct tgcaagatgc gtatcgagca gctgaagcag
     2461 accatctgca agggcaacga ggaaatggag aagaactctg aaggactgct gaagactaag
     2521 gaaaagaacc agaagctgta ctcacgcgct cagcgtcacc aggagaagaa ggaaaagatc
     2581 cagaggcaca acagaaagct gggtgacctg gtggagaaga agaccatcga cctgcgcagc
     2641 cactacgaac gtctggccaa cctgagacgc tcccacatcc tggagctgac cagcgtcatc
     2701 ttccccatcg aggaagtgaa gactggcgtc agggacccag ctgacgtgtc cagcgagagc
     2761 gactctgcca tgacctcttc aactgtctcc aagctggctg aagcccgtag gaccacttac
     2821 ctgagcggac gctgggtgtg cgacgaccac aacggagaca cctcaatctc catcactggt
     2881 ccctggatct ccctgccaaa caacggagac tactctgctt actactcatg ggtcgaggaa
     2941 aagaagacca ctcagggtcc tgacatggag cagtctaacc ccgcttacac catctcagct
     3001 gccctgtgct acgccactca gctggtgaac atcctgtctc acatcctgga cgtcaacctg
     3061 ccaaagaagc tgtgcaactc cgagttctgc ggcgaaaacc tgagcaagca gaagttcacc
     3121 cgcgctgtga agaagctgaa cgccaacatc ctgtacctgt gcttctcaca gcacgtcaac
     3181 ctggaccagc tgcaacctct gcacactctg cgtaacctga tgtacctggt gtctccatcc
     3241 agcgagcacc tgggaaggtc aggtccattc gaagtcagag ctgacctgga ggaaagcatg
     3301 gagttcgtgg accctggcgt cgccggagag tctgacgaat caggtgacga gagggtgtcc
     3361 gacgaggaaa ccgacctggg cactgactgg gaaaacctgc caagccctag attctgcgac
     3421 atccccagcc agtctgtgga agtctcacaa tctcagagca cccaggcctc ccctcccatc
     3481 gcttcttcat ccgccggtgg catgatctcg tcagccgcag catccgtcac ttcgtggttc
     3541 aaggcataca ctggacatag gtaacgccat taacctgatg ttctggggaa tataagcttg
     3601 cggccgcact cgagcaccac caccaccacc actgacatgc ggtaccaagc ttgtcgagaa
     3661 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     3721 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3781 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3841 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3901 tgtctggatc tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg
     3961 aagctgagtt ggctgctgcc accgctgagc aataactatc ataaccccta gggtataccc
     4021 atctaattgg actatgagac caaccagata agtgaaatct agttccaaac tattttgtca
     4081 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     4141 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     4201 gcccacaacc ggttgacttg ggtcaactgt cagaccaagt ttactcatat atactttaga
     4261 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4321 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4381 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4441 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4501 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     4561 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4621 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4681 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4741 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4801 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4861 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4921 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4981 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5041 acatgttctt tcctgcgtta tcccctgatt gacttgggtc gctcttcctg tggatgcg
//
