LOCUS       sequence-374446-       17204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the expression and purification of 
            GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda 
            cells (Sf9). Internal reference: SMC1327.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17204)
  AUTHORS   Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, 
            Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn 
            M, Lindblom RSJ, Martens S, Lazarou M
  TITLE     Unconventional initiation of PINK1/Parkin mitophagy by Optineurin.
  JOURNAL   Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 
            10.1016/j.molcel.2023.04.021.
  PUBMED    37207627
REFERENCE   2  (bases 1 to 17204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.04"; journalName: "Mol Cell"; date: 
            "2023-05-18- 18"; volume: "83"; issue: "10"; pages: "1693-1709.e9"
COMMENT     Sequence Label: pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1
FEATURES             Location/Qualifiers
     source          1..17204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(49..582)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(771..776,777..793,794..799))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        987..1078
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1134..1787
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1745..1767
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1794..1817
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1818..2522
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1960..1978)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2228..2247
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4070..4087
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4231..4365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4537..4628
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     RBS             6069..6077
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7398..7415
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    7559..7693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7865..7956
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             12139..12156
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    12300..12434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        12606..12697
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             14156..14173
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    14317..14451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  14611..14776
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     primer_bind     complement(15047..15066)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(15132..15947)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(15132..15947)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     15855..15874
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     15939..15958
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      16069..16657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16558..16577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16811..16828
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  16962..17186
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 accgaacaac tccgcggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt
       61 acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc
      121 cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt
      181 ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc
      241 ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac
      301 gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt
      361 cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc
      421 tacgtctccg aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc
      481 agggccgagc ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg
      541 gcgactgccc tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg ctccataaca
      601 tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta
      661 caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
      721 cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agtttacgaa
      781 ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc
      841 cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca
      901 aggtttcacc tgtattcagt cgactggtac caacactgcg tttaaacact agtatcgatt
      961 cgcgacctac tccggaatat taatagatca tggagataat taaaatgata accatctcgc
     1021 aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat
     1081 tccggattat tcataccgtc ccaccatcgg gcgacggatc ccggtccgaa accatgtccc
     1141 ctatactagg ttattggaaa attaagggcc ttgtgcaacc cactcgactt cttttggaat
     1201 atcttgaaga aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat aaatggcgaa
     1261 acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc ttattatatt gatggtgatg
     1321 ttaaattaac acagtctatg gccatcatac gttatatagc tgacaagcac aacatgttgg
     1381 gtggttgtcc aaaagagcgt gcagagattt caatgcttga aggagcggtt ttggatatta
     1441 gatacggtgt ttcgagaatt gcatatagta aagactttga aactctcaaa gttgattttc
     1501 ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat aaaacatatt
     1561 taaatggtga tcatgtaacc catcctgact tcatgttgta tgacgctctt gatgttgttt
     1621 tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt aaaaaacgta
     1681 ttgaagctat cccacaaatt gataagtact tgaaatccag caagtatata gcatggcctt
     1741 tgcagggctg gcaagccacg tttggtggtg gcgaccatcc tccaaaatcg gatctggaag
     1801 ttctgttcca ggggcccgtg agcaagggcg aggaggataa catggccatc atcaaggagt
     1861 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     1921 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     1981 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     2041 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     2101 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     2161 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     2221 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     2281 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     2341 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     2401 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     2461 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     2521 agggttccgg ctctggtagc atggcatcgc caagcggaaa aggagcaaga gccctggaag
     2581 cacccggctg cggaccaaga ccactcgccc gtgacctcgt tgatagcgtg gacgacgctg
     2641 agggtctgta cgtggccgtc gaacgttgcc ctctgtgcaa caccactcgc cgtaggctga
     2701 cctgcgctaa gtgcgtgcag agcggtgact tcgtctactt cgacggacgc gaccgcgagc
     2761 gtttcatcga caagaaggaa aggctgtcca gactgaagag caagcaggag gagttccaga
     2821 aggaggtcct gaaggctatg gagggcaagt ggatcactga ccagctgcgc tggaagatca
     2881 tgtcttgcaa gatgcgtatc gagcagctga agcagaccat ctgcaagggc aacgaggaaa
     2941 tggagaagaa ctctgaagga ctgctgaaga ctaaggaaaa gaaccagaag ctgtactcac
     3001 gcgctcagcg tcaccaggag aagaaggaaa agatccagag gcacaacaga aagctgggtg
     3061 acctggtgga gaagaagacc atcgacctgc gcagccacta cgaacgtctg gccaacctga
     3121 gacgctccca catcctggag ctgaccagcg tcatcttccc catcgaggaa gtgaagactg
     3181 gcgtcaggga cccagctgac gtgtccagcg agagcgactc tgccatgacc tcttcaactg
     3241 tctccaagct ggctgaagcc cgtaggacca cttacctgag cggacgctgg gtgtgcgacg
     3301 accacaacgg agacacctca atctccatca ctggtccctg gatctccctg ccaaacaacg
     3361 gagactactc tgcttactac tcatgggtcg aggaaaagaa gaccactcag ggtcctgaca
     3421 tggagcagtc taaccccgct tacaccatct cagctgccct gtgctacgcc actcagctgg
     3481 tgaacatcct gtctcacatc ctggacgtca acctgccaaa gaagctgtgc aactccgagt
     3541 tctgcggcga aaacctgagc aagcagaagt tcacccgcgc tgtgaagaag ctgaacgcca
     3601 acatcctgta cctgtgcttc tcacagcacg tcaacctgga ccagctgcaa cctctgcaca
     3661 ctctgcgtaa cctgatgtac ctggtgtctc catccagcga gcacctggga aggtcaggtc
     3721 cattcgaagt cagagctgac ctggaggaaa gcatggagtt cgtggaccct ggcgtcgccg
     3781 gagagtctga cgaatcaggt gacgagaggg tgtccgacga ggaaaccgac ctgggcactg
     3841 actgggaaaa cctgccaagc cctagattct gcgacatccc cagccagtct gtggaagtct
     3901 cacaatctca gagcacccag gcctcccctc ccatcgcttc ttcatccgcc ggtggcatga
     3961 tctcgtcagc cgcagcatcc gtcacttcgt ggttcaaggc atacactgga cataggtaac
     4021 gccattaacc tgatgttctg gggaatataa gcttgcggcc gcactcgagc accaccacca
     4081 ccaccactga catgcggtac caagcttgtc gagaagtact agaggatcat aatcagccat
     4141 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     4201 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     4261 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4321 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag
     4381 cctagaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     4441 tgagcaataa ctatcataac ccctagggta tacccatcta attggactag tttaaacact
     4501 agtatcgatt cgcgacctac tccggaatat taatagatca tggagataat taaaatgata
     4561 accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac
     4621 ctataaatat tccggattat tcataccgtc ccaccatcgg gcgacggatc ccggtccgaa
     4681 accatgggcg aagcggagaa attccactac atctactctt gcgacttgga catcaacgtt
     4741 caactcaaaa tcggttcact cgaaggtaaa cgcgagcaga agtcctacaa ggccgtgctg
     4801 gaagacccta tgctgaagtt ctcaggtctg taccaggaaa cctgctccga cctgtacgtg
     4861 acttgccagg tcttcgctga gggcaagcct ctggctctgc ccgtgcgcac cagctacaag
     4921 gctttctcta ctcgttggaa ctggaacgag tggctgaagc tgcccgtcaa gtaccccgac
     4981 ctgccacgta acgctcaggt ggccctgacc atctgggacg tgtacggtcc cggcaaggcc
     5041 gtgccagtcg gtggcaccac tgtgtcactg ttcggcaagt acggaatgtt ccgccaggga
     5101 atgcacgacc tgaaagtgtg gccaaacgtc gaggctgacg gctccgaacc aaccaagact
     5161 cctggacgta cctccagcac tctgtcagag gaccagatgt ccaggctggc taagctgacc
     5221 aaggcccaca gacagggtca catggtgaag gtggactggc tggaccgcct gactttccgt
     5281 gagatcgaaa tgatcaacga gagcgaaaag cgctcttcaa acttcatgta cctgatggtg
     5341 gagttccgtt gcgtcaagtg cgacgacaag gaatacggta tcgtgtacta cgagaaggac
     5401 ggcgacgaat ccagccctat cctgacctct ttcgagctgg tgaaggtccc agaccctcag
     5461 atgagcatgg agaacctggt cgaatctaag caccacaagc tggctcgctc cctgcgtagc
     5521 ggtccatctg accacgacct gaagccaaac gctgccacta gggaccagct gaacatcatc
     5581 gtgagctacc ctcccaccaa gcagctgact tacgaggaac aggacctggt ctggaagttc
     5641 agatactacc tgaccaacca ggagaaggct ctgactaagt tcctgaagtg cgtgaactgg
     5701 gacctgccac aggaggctaa gcaggccctg gaactgctgg gcaagtggaa gccaatggac
     5761 gtcgaggact ctctggaact gctgtcttca cactacacca acccaactgt gcgccgttac
     5821 gctgtcgcca ggctgagaca ggccgacgac gaagacctgc tgatgtacct gctgcaactg
     5881 gtgcaggctc tgaagtacga gaacttcgac gacatcaaga acggactgga acctaccaag
     5941 aaggacagcc agtccagcgt gtctgagaac gtctctaact caggtatcaa ctcagccgaa
     6001 atcgactctt cacagatcat cacctcaccc ctgccatccg tgtccagccc acctcccgct
     6061 tccaagacta aggaggtccc cgacggcgag aacctggaac aggacctgtg caccttcctg
     6121 atcagccgcg cttgcaagaa ctctactctg gccaactacc tgtactggta cgtgatcgtc
     6181 gagtgcgaag accaggacac ccagcagcgt gacccaaaga ctcacgaaat gtacctgaac
     6241 gtgatgagga gattctccca ggctctgctg aagggagaca agtcagtgag ggtcatgaga
     6301 tccctgctgg ctgcccagca gaccttcgtg gaccgcctgg tccacctgat gaaggccgtc
     6361 cagagggaga gcggaaaccg caagaagaag aacgaacgtc tgcaagctct gctgggtgac
     6421 aacgagaaga tgaacctgtc tgacgtggag ctgatccctc tgcccctgga acctcaggtc
     6481 aagatcaggg gtatcatccc tgaaaccgct actctgttca agtcagctct gatgccagcc
     6541 cagctgttct tcaagactga agacggtggc aagtaccctg tgatcttcaa gcacggcgac
     6601 gacctgaggc aggaccagct gatcctgcaa atcatctccc tgatggacaa gctgctgaga
     6661 aaggagaacc tggacctgaa gctgacccct tacaaggtgc tggccaccag cactaagcac
     6721 ggcttcatgc agttcatcca gtctgtgccc gtcgctgagg tcctggacac tgaaggaagc
     6781 atccagaact tcttccgtaa gtacgctcct agcgagaacg gtcccaacgg catctctgcc
     6841 gaagtgatgg acacctacgt caagtcttgc gctggctact gcgtgatcac ttacatcctg
     6901 ggagtcggtg acaggcacct ggacaacctg ctgctgacca agactggaaa gctgttccac
     6961 atcgacttcg gatacatcct gggtcgcgac cctaagcctc tgccacctcc catgaagctg
     7021 aacaaggaga tggtggaggg tatgggcgga acccagtcag agcagtacca ggagttcagg
     7081 aagcagtgct acactgcctt cctgcacctg cgccgttact caaacctgat cctgaacctg
     7141 ttctccctga tggtggacgc taacatccca gacatcgccc tggagcctga caagaccgtg
     7201 aagaaggtgc aagacaagtt cagactggac ctgtccgacg aggaagctgt gcactacatg
     7261 cagtcactga tcgacgaatc cgtccacgca ctcttcgcag cggtcgttga gcagattcat
     7321 aagttcgcac aatactggag aaaataacgc cattaacctg atgttctggg gaatataagc
     7381 ttgcggccgc actcgagcac caccaccacc accactgaca tgcggtacca agcttgtcga
     7441 gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     7501 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     7561 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7621 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7681 tcatgtctgg atctgatcac tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa
     7741 aggaagctga gttggctgct gccaccgctg agcaataact atcataaccc ctagggtata
     7801 cccatctaat tggcctggtt taaacactag tatcgattcg cgacctactc cggaatatta
     7861 atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt
     7921 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc
     7981 accatcgggc gacggatccc ggtccgaaac catgggtaac cagttggcag gcatcgcccc
     8041 ctcgcagatt ttgagcgttg agtcctactt ctccgacatc cacgatttcg aatacgacaa
     8101 gtctctgggc tcaaccagat tcttcaaggt ggctagagcc aagcaccgcg aaggactggt
     8161 ggtcgtgaag gtcttcgcca tccaggaccc taccctgccc ctgacttctt acaagcagga
     8221 gctggaggaa ctgaagatcc gcctgaactc agctcagaac tgcctgccat tccagaaggc
     8281 ctccgagaag gctagcgaaa aggctgccat gctgttccgt cagtacgtga gggacaacct
     8341 gtacgacaga atctctaccc gccctttcct gaacaacatc gaaaagagat ggatcgcttt
     8401 ccagatcctg actgccgtgg accaggctca caagtcagga gtccgccacg gtgacatcaa
     8461 gaccgagaac gtcatggtga cttcttggaa ctgggtgctg ctgaccgact tcgcctcatt
     8521 caagccaact tacctgcctg aagacaaccc cgctgacttc aactacttct tcgacacctc
     8581 ccgccgtagg acttgctaca tcgcccccga gagattcgtg gacggtggca tgttcgctac
     8641 cgagctggaa tacatgcgcg acccatccac tcctctggtg gacctgaact ccaaccagcg
     8701 taccaggggt gaactgaaga gggctatgga catcttctcc gctggttgcg tgatcgctga
     8761 gctgttcact gaaggcgtcc ccctgttcga cctgtcacag ctgctggctt accgtaacgg
     8821 ccacttcttc ccagagcagg tgctgaacaa gatcgaggac cactccatca gggaactggt
     8881 cacccagatg atccaccgtg aaccagacaa gaggctggag gccgaagact acctgaagca
     8941 gcagcgcggc aacgctttcc cagaaatctt ctacaccttc ctgcaacctt acatggctca
     9001 gttcgccaag gagactttcc tgagcgccga cgaacgcatc ctggtcatcc gcaaggacct
     9061 gggtaacatc atccacaacc tgtgcggcca cgacctgcct gaaaaggctg agggcgaacc
     9121 caaggagaac ggactggtca tcctggtgtc cgtgatcacc tcatgcctgc aaactctgaa
     9181 gtactgcgac tccaagctgg ctgccctgga actgatcctg cacctggctc ctagactgag
     9241 cgtggagatc ctgctggacc gcatcacccc ctacctgctg cacttctcca acgacagcgt
     9301 cccaagagtg cgcgctgagg ccctgcgtac cctgactaag gtcctggccc tggtcaagga
     9361 agtgcccagg aacgacatca acatctaccc tgagtacatc ctgcccggaa tcgctcacct
     9421 ggcccaggac gacgctacca tcgtgcgtct ggcttacgcc gagaacatcg ctctgctggc
     9481 cgaaactgct ctgaggttcc tggagctggt gcagctgaag aacctgaaca tggaaaacga
     9541 ccctaacaac gaggaaatcg acgaggtcac ccaccccaac ggtaactacg acactgagct
     9601 gcaagctctg cacgaaatgg tgcagcagaa ggtcgtgacc ctgctgtctg acccagaaaa
     9661 catcgtcaag cagaccctga tggagaacgg aatcactcgt ctgtgcgtgt tcttcggtag
     9721 gcagaaggcc aacgacgtcc tgctgtccca catgatcact ttcctgaacg acaagaacga
     9781 ctggcacctg cgtggcgctt tcttcgacag catcgtcggt gtggctgcct acgtgggctg
     9841 gcagtccagc tctatcctga agcccctgct gcaacagggt ctgtccgacg ccgaggagtt
     9901 cgtcatcgtg aaggccctgt acgctctgac ctgcatgtgc cagctgggtc tgctgcaaaa
     9961 gccacacgtg tacgagttcg cctccgacat cgctccattc ctgtgccacc ctaacctgtg
    10021 gatcagatac ggcgctgtgg gattcatcac cgtcgtggcc cgccagatca gcactgctga
    10081 cgtctactgc aagctgatgc catacctgga cccttacatc acccagccta tcatccagat
    10141 cgaaagaaag ctggtgctgc tgtccgtcct gaaggagccc gtgtctcgtt caatcttcga
    10201 ctacgctctg aggtctaagg acatcacttc actgttccgc cacctgcaca tgcgtcagaa
    10261 gaagaggaac ggatctctgc ctgactgccc tcccccagag gacccagcta tcgctcagct
    10321 gctgaagaag ctgctgtcac agggtatgac cgaggaagag gaagacaagc tgctggccct
    10381 gaaggacttc atgatgaagt ccaacaaggc taaggccaac atcgtggacc agagccacct
    10441 gcacgactca tcccagaagg gtgtcatcga cctggctgcc ctgggtatca ccggcagaca
    10501 ggtggacctg gtgaagacta agcaggagcc tgacgacaag cgtgctagga agcacgtgaa
    10561 gcaggactcc aacgtcaacg aggaatggaa gtccatgttc ggcagcctgg accctcccaa
    10621 catgcctcag gccctgccaa agggcagcga ccaggaagtg atccagaccg gaaagccacc
    10681 tcgttctgag tcctccgctg gtatctgcgt ccctctgtca acttcatccc aggtccccga
    10741 ggtgaccact gtccagaaca agaagcccgt gatcccagtc ctgtcctcca ccatcctgcc
    10801 aagcacttac cagatccgta tcaccacttg caagaccgag ctgcaacagc tgatccagca
    10861 gaagagagag cagtgcaacg ccgaacgcat cgctaagcag atgatggaga acgccgagtg
    10921 ggaatccaag cccccacctc ccggatggag gcctaagggt ctgctggtgg cccacctgca
    10981 cgaacacaag agcgctgtga acagaatccg cgtctctgac gagcactcac tgttcgctac
    11041 ctgctctaac gacggcactg tcaagatttg gaactcccag aagatggagg gaaagaccac
    11101 taccactcgc tccatcctga cctacagcag aatcggaggt cgcgtgaaga ccctgacttt
    11161 ctgccagggc tcccactacc tggctatcgc cagcgacaac ggagccgtcc agctgctggg
    11221 tatcgaggct tccaagctgc ctaagtcacc caagatccac cctctgcaat ctcgtatcct
    11281 ggaccagaag gaggacggat gcgtcgtgga catgcaccac ttcaactccg gtgctcagag
    11341 cgtgctggct tacgccactg tgaacggctc actggtcgga tgggacctga ggtcatccag
    11401 caacgcctgg accctgaagc acgacctgaa gtccggactg atcactagct tcgctgtgga
    11461 catccaccag tgctggctgt gcatcggcac ctcctccggc actatggcct gctgggacat
    11521 gcgtttccag ctgccaatct ccagccactg ccacccttcc cgtgctagga tcagacgcct
    11581 gtctatgcac cccctgtacc agtcatgggt catcgctgcc gtccagggta acaacgaagt
    11641 gtctatgtgg gacatggaaa ccggcgaccg taggttcact ctgtgggcct cttcagctcc
    11701 acctctgtca gagctgcaac cctctccaca ctcagtccac ggaatctact gctcccccgc
    11761 cgacggtaac ccaatcctgc tgaccgctgg cagcgacatg aagatccgtt tctgggacct
    11821 ggcctaccca gagaggtctt acgtcgtggc tggttcaacc tccagccctt ccgtgagcta
    11881 ctaccgcaag atcatcgagg gaactgaagt cgtgcaggaa atccagaaca agcagaaggt
    11941 cggtccttct gacgacactc ctagaagggg accagagagc ctgccagtgg gacaccacga
    12001 catcatcacc gacgtcgcta ctttccagac tacgcaagga ttcattgtga cagcaagcag
    12061 ggatggtatc gttaaagtct ggaaataacg ccattaacct gatgttctgg ggaatataag
    12121 cttgcggccg cactcgagca ccaccaccac caccactgac atgcggtacc aagcttgtcg
    12181 agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
    12241 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
    12301 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    12361 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    12421 atcatgtctg gatctgatca ctgcttgagc ctagaagatc cggctgctaa caaagcccga
    12481 aaggaagctg agttggctgc tgccaccgct gagcaataac tatcataacc cctagggtat
    12541 acccatctaa ttggcggagt ttaaacacta gtatcgattc gcgacctact ccggaatatt
    12601 aatagatcat ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg
    12661 ttttcgtaac agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc
    12721 caccatcggg cgacggatcc cggtccgaaa ccatggaggg ttctaagacc agcaacaact
    12781 cgactatgca agtgtccttc gtgtgtcaga gatgtagcca gcctttgaaa ttggatacga
    12841 gcttcaagat cctggacagg gtcaccatcc aggagctgac tgctcctctg ctgaccactg
    12901 ctcaggccaa gcccggtgaa acccaggagg aagagactaa cagcggcgaa gagcccttca
    12961 tcgaaacccc tcgccaggac ggagtgtctc gccgtttcat ccctcccgct agaatgatgt
    13021 ctaccgagtc agccaactcc ttcactctga tcggcgaggc cagcgacggt ggtactatgg
    13081 agaacctgtc taggagactg aaggtcactg gcgacctgtt cgacatcatg tcaggacaga
    13141 ccgacgtgga ccaccccctg tgcgaagagt gcaccgacac tctgctggac cagctggaca
    13201 cccagctgaa cgtcactgag aacgaatgcc agaactacaa gcgttgcctg gagatcctgg
    13261 aacagatgaa cgaggacgac tccgaacagc tgcaaatgga gctgaaggaa ctggctctgg
    13321 aagaggaacg cctgatccag gagctggaag acgtggagaa gaaccgtaag atcgtcgctg
    13381 agaacctgga aaaggtgcag gctgaggccg aacgcctgga ccaggaggaa gcccagtacc
    13441 agagggagta ctcagagttc aagcgccagc agctggagct ggacgacgaa ctgaagtccg
    13501 tcgagaacca gatgcgttac gctcagactc agctggacaa gctgaagaag accaacgtgt
    13561 tcaacgccac tttccacatc tggcacagcg gacagttcgg caccatcaac aacttcaggc
    13621 tgggtagact gccatctgtg cctgtcgagt ggaacgaaat caacgctgcc tggggccaga
    13681 ctgtcctgct gctgcacgct ctggccaaca agatgggact gaagttccag cgctaccgtc
    13741 tggtgccata cggtaaccac agctacctgg agtctctgac cgacaagtca aaggaactgc
    13801 ctctgtactg ctccggaggt ctgcgcttct tctgggacaa caagttcgac cacgctatgg
    13861 tggccttcct ggactgcgtc cagcagttca aggaggaagt cgagaagggc gaaactcgct
    13921 tctgcctgcc ttaccgtatg gacgtggaga agggaaagat cgaagacacc ggcggatcag
    13981 gtggctccta cagcatcaag acccagttca actccgagga acagtggact aaggccctga
    14041 agttcatgct cacaaacctg aagtggggat tggcgtgggt gtcatcgcag ttctacaaca
    14101 aataacgcca ttaacctgat gttctgggga atataagctt gcggccgcac tcgagcacca
    14161 ccaccaccac cactgacatg cggtaccaag cttgtcgaga agtactagag gatcataatc
    14221 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    14281 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    14341 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    14401 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg
    14461 cttgagccta gaagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
    14521 caccgctgag caataactat cataacccct agggtatacc catctaattg gggtaggctg
    14581 agtaggcaag atgttctggt atgtttaaac aaccagataa gtgaaatcta gttccaaact
    14641 attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt
    14701 tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact
    14761 attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttttaatc aaacatcctg
    14821 ccaactccat gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat
    14881 ttttctgtca tctcttcgtt attaatgttt gtaattgact gaatatcaac gcttatttgc
    14941 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    15001 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    15061 ttcccttcct ttctcgccac gttcgccaaa cgtactgcgg taaagctcat cagcgtggtc
    15121 gtgaagaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
    15181 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
    15241 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
    15301 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
    15361 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
    15421 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
    15481 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
    15541 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
    15601 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
    15661 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
    15721 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
    15781 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
    15841 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
    15901 tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcataac accccttgta
    15961 ttactgttta tgtaagcaga cagttttatt gttcatgacc aaaatccctt aacgtgagtt
    16021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    16081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    16141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    16201 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    16261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    16321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    16381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    16441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    16501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    16561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    16621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    16681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    16741 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    16801 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
    16861 ccttacgcat ctgtgcggta tttcacaccg catagaccag ccgcgtaacc tggcaaaatc
    16921 ggttacggtt gagtaataaa tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt
    16981 cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag
    17041 ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc
    17101 taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg
    17161 ccaagggcat ggtaaagact atattcgcgg cgttgtgaca attt
//
