LOCUS       sequence-374452-        8786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in 
            Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, 
            Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn 
            M, Lindblom RSJ, Martens S, Lazarou M
  TITLE     Unconventional initiation of PINK1/Parkin mitophagy by Optineurin.
  JOURNAL   Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 
            10.1016/j.molcel.2023.04.021.
  PUBMED    37207627
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.04"; journalName: "Mol Cell"; date: 
            "2023-05-18- 18"; volume: "83"; issue: "10"; pages: "1693-1709.e9"
COMMENT     Sequence Label: pFastBac_Dual_GST-TEV-EGFP-TBK1
FEATURES             Location/Qualifiers
     source          1..8786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(19..729,730..732,733..735))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(61..82)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     410..429
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     669..690
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(742..762)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(772..789)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(796..1452)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MASPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIR
                     YGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVV
                     LYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(816..838)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     promoter        complement(1495..1586)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(1553..1570)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        1610..1719
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     primer_bind     complement(1811..1830)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1855..1903
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        join(2411..2416,2417..2433,2434..2439)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2628..3161
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  3228..3452
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(3586..3603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3757..4345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3837..3856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4516..5307,5308..5376))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5139..5158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5377..5481)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5507..5961)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5644..5665)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5855..5874
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  6145..6310
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    6339..6473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6363..6382)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6417..6436
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ttgcatgcct ccaccgccct tgtacagctc gtccatgccg agagtgatcc cggcggcggt
       61 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga
      121 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
      181 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
      241 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
      301 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
      361 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
      421 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
      481 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt
      541 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
      601 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
      661 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
      721 gcccttgctc accatgcgcg ctccctgaaa gtataaattc tctcctgatc ctgatccacg
      781 cggaaccaga tccgattttg gaggatggtc gccaccacca aacgtggctt gccagccctg
      841 caaaggccat gctatatact tgctggattt caagtactta tcaatttgtg ggatagcttc
      901 aatacgtttt ttaaaacaaa ctaattttgg gaacgcatcc aggcacattg ggtccatgta
      961 taaaacaaca tcaagagcgt catacaacat gaagtcagga tgggttacat gatcaccatt
     1021 taaatatgtt ttatgacata aacgatcttc gaacattttc agcatttcag gtagcttgct
     1081 aagaaaatca actttgagag tttcaaagtc tttactatat gcaattctcg aaacaccgta
     1141 tctaatatcc aaaaccgctc cttcaagcat tgaaatctct gcacgctctt ttggacaacc
     1201 acccaacatg ttgtgcttgt cagctatata acgtatgatg gccatagact gtgttaattt
     1261 aacatcacca tcaatataat aaggaagatt gggaaactcc aaacccaatt caaacttttt
     1321 gtttcgccat ttatcacctt catcgcgctc atacaaatgc tcttcatatt tttcttcaag
     1381 atattccaaa agaagtcgag tgggttgcac aaggccctta attttccaat aacctagtat
     1441 aggggatgcc atggatccgc gcccgatggt gggacggtat gaataatccg gaatatttat
     1501 aggttttttt attacaaaac tgttacgaaa acagtaaaat acttatttat ttgcgagatg
     1561 gttatcattt taattatctc catgatctat taatattccg gagtatacgg acctttaatt
     1621 caacccaaca caatatatta tagttaaata agaattatta tcaaatcatt tgtatattaa
     1681 ttaaaatact atactgtaaa ttacatttta tttacaatca ctcgacgaag acttgatcac
     1741 ccgggatctc gagccatggt gctagcagct gatgcatagc atgcggtacc gggagatggg
     1801 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     1861 taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg
     1921 tcccagggct ggcactctgt cgatacccca ccgagacccc attgggacca atacgcccgc
     1981 gtttcttcct tttccccacc ccaaccccca agttcgggtg aaggcccagg gctcgcagcc
     2041 aacgtcgggg cggcaagccc tgccatagcc actacgggta cgtagccaac cactagaact
     2101 atagctagag tcctgggcga acaaacgatg ctcgccttcc agaaaaccga ggatgcgaac
     2161 cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc taccgatctc
     2221 ctgaagcaag ggcagatccg tgcacagcac cttgccgtag aagaacagca aggccgccaa
     2281 tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg acagaaatgc
     2341 ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     2401 acgaacccag ttgacataag cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg
     2461 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     2521 tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat ccaagcagca
     2581 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     2641 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg
     2701 gcatcattcg cacatgtagg ctcggccctg accaagtcaa atccatgcgg gctgctcttg
     2761 atcttttcgg tcgtgagttc ggagacgtag ccacctactc ccaacatcag ccggactccg
     2821 attacctcgg gaacttgctc cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag
     2881 aagcggttgt tggcgctctc gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg
     2941 agatctatat ctatgatctc gcagtctccg gcgagcaccg gaggcagggc attgccaccg
     3001 cgctcatcaa tctcctcaag catgaggcca acgcgcttgg tgcttatgtg atctacgtgc
     3061 aagcagatta cggtgacgat cccgcagtgg ctctctatac aaagttgggc atacgggaag
     3121 aagtgatgca ctttgatatc gacccaagta ccgccaccta acaattcgtt caagccgaga
     3181 tcggcttccc ggccgcggag ttgttcggta aattgtcaca acgccgcgaa tatagtcttt
     3241 accatgccct tggccacgcc cctctttaat acgacgggca atttgcactt cagaaaatga
     3301 agagtttgct ttagccataa caaaagtcca gtatgctttt tcacagcata actggactga
     3361 tttcagttta caactattct gtctagttta agactttatt gtcatagttt agatctattt
     3421 tgttcagttt aagactttat tgtccgccca cacccgctta cgcagggcat ccatttatta
     3481 ctcaaccgta accgattttg ccaggttacg cggctggtct atgcggtgtg aaataccgca
     3541 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     3601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     5521 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     5581 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     5641 gttccagttt ggaacaagag tccactatta agaacgtgga ctccaacgtc aaagggcgaa
     5701 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     5761 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     5821 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     5881 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     5941 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcaaataagc gttgatattc
     6001 agtcaattac aaacattaat aacgaagaga tgacagaaaa attttcattc tgtgacagag
     6061 aaaaagtagc cgaagatgac ggtttgtcac atggagttgg caggatgttt gattaaaaac
     6121 ataacaggaa gaaaaatgcc ccgctgtggg cggacaaaat agttgggaac tgggaggggt
     6181 ggaaatggag tttttaagga ttatttaggg aagagtgaca aaatagatgg gaactgggtg
     6241 tagcgtcgta agctaatacg aaaattaaaa atgacaaaat agtttggaac tagatttcac
     6301 ttatctggtt cggatctcct aggctcaagc agtgatcaga tccagacatg ataagataca
     6361 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     6421 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     6481 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     6541 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagtactt ctcgacaagc
     6601 ttttacagac agtccacgtt tctcagtcca ccatccatcg tcaggctacc gaatctttcc
     6661 aagatgtggt tgttctcagc cagttccttg accacgccct ccatttcctc cttcagcttc
     6721 ttcataccca gagtcatttc caccagggtg ttggatgaag ggtagattgg ggtcatagtg
     6781 tgcttgatac cagagctggc agtgaacatc ttctgaggca gagtctcttg cagctcgttg
     6841 gtgtactcct ggtacttgct cacttcctct tcgatgtcga agcactggtt ggtcagggac
     6901 agcagctgct ttctcaggtg cagcatcttc ctgatccact cttcgctctt gttcaggaaa
     6961 gcctcgtact tcttgacgca ttcgtcggtg aagtgagtca tagccttggt ggcgtggtag
     7021 tacagcttct gcttgtcgaa cttgtggatc tgctcttcgt tgtaagccag acggcgctcg
     7081 gccttgtcct tcttgaactg gtagtagatt tcagtcatgc agttcagcag gacttgcagc
     7141 ttctccacgt tcctgtcctt agggtgggtg ccttcctggt gagcccaagc gtcagccagt
     7201 gaacctcctg ggctcagacg ggagtcgatg tcttgcaggg aggtttcgat agtgccctgg
     7261 gatgaagaca ggcgcagcag cttagtgtgg atgtcgctga tctctcccag ttcggcagcc
     7321 tccaggttga tcttcatcag cttctcgtag accttcacgg tcttttcgat gttacggatg
     7381 cagaagtcca gagtgatgac cacctcggtc ttcttgtgca cagtttcgtt gtagtcgtcc
     7441 ttgatcagct cgatcagcca acggattccc ttgcgcatca gttcctggta cagcagcaga
     7501 gttgaagcga tgcggcaagc gtagcagacc actccggtga tggccttagc catgctagcg
     7561 tcgccgtcca ggtcgtaacg tgggtggacc ttgggcaggc tgatcttctc gtagatcagg
     7621 ccgatagtgt tcagtggctc gcgggacacc acgaagatag ggttctcttc ggtagtcttt
     7681 gggaagtgct gagccaggcg accgggctcc agcaccagtc tccttccctc gtagatcagt
     7741 tcctggttgc tggagatgat cttggtctgc ttgtagacca gttcgtggaa gatagtagca
     7801 gtgttgtaag agtggatgta aatcttgtgg gcagtcatct gttgcaggct gaagacgtgg
     7861 atgaccattc tgtgcaggat gtcggaggtt tcagcgaaga actggtcgaa accccagcac
     7921 ttctcctggt cagcttccag gatgttggcc aggactgggg tcagcagcac ttgcagaccg
     7981 cgggacagag agcagctcac tggcatgtcg ccgctccagt cgatgggacc gttttcagcc
     8041 ttctggactc ctgagatggc gccagagggc ttgccagtga tgatcttgta catgacctcc
     8101 ttgttacggc gaggaccttc gaaggggcgg aaaggcagtg atccagtggc agcgtggtag
     8161 aaggtgacgc cgatagacca caggtccacg gtagcaccgt acttcttctg gtggtccttt
     8221 ctcagcacgg ccctctcgta catgtcaggg tgcaggtact cttcagttcc gtacagagag
     8281 acgaactgtt cgtcgtcttc cagctctctg gcagcgccga agtcggtcag cttgtagacg
     8341 ctctgtccgt cctcgccgat cacgcgcatg atgtttcctg gcttgatgtc tctgtgcacg
     8401 atgccgtttt ccctcaggtg gttcatgcca ccgaccacgt cacgcaggac gatcaggaac
     8461 tcgctctcgg gcagaccgta agcgttggaa ggctcttcca ggacagtgta cagggaaccg
     8521 caggggcaga actccatgat cagcaccttg tggcgggtag tggtttcttc ctcgatggcg
     8581 aacagcttga cgatgttctt gtggttcagc ttcttcagca cttcgaactc acgcatctgg
     8641 acgtccacag ggcgcaggaa tgagatgttg ttgaacacct tgatggcgaa caggtcaccg
     8701 gtcttcttgt gccttcccct gaacacgtta gcggtagcgc cttgtccgag aatgtccgag
     8761 aggagccaga ggtggttgga tgtgga
//
