LOCUS       sequence-374462-       13034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of NEK1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13034)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 13034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-NEK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..13034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4170..4190
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4190..5401
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5238..5257
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5405..5425)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5426..5435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5438..6208
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6215..6256
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6257..6281
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9858..9882)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9912..10500
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9965..9985)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10383..10394)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10514..11013
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10549..10572)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10946..10965
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11034..11633
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11034..11053)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11530..11550
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             11771..12004
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12076..12197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12113..12132)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12167..12186
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12237..12372
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12392..12411)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12393..12411)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12421..12438)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12421..12437)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12430..12452)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12579..13007
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12666..12685)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12876..12897
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcac aactttgtat agaaaagttg gcctccgcgc
     4201 cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac
     4261 gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc
     4321 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg
     4381 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc
     4441 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag
     4501 gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg
     4561 tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt
     4621 tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta
     4681 gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg
     4741 cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa
     4801 caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg
     4861 gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt
     4921 tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc
     4981 cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc
     5041 cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt
     5101 tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc
     5161 ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac
     5221 ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt
     5281 gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt
     5341 tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga
     5401 cggccaactt ttgtatacaa agtgggccac catgggaatc ccgctgctga acgctaaaca
     5461 gattctggga cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa
     5521 tcagtacctg ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata
     5581 tcagcaggca gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct
     5641 gtacctgagt atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt
     5701 catcggaatt gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa
     5761 atggcccaat gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc
     5821 cggaataaca ggcgatgctg cacagatcgt cattggcgcc gggattaacg tggctatgag
     5881 gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag cagggattaa
     5941 cctggacagg aatactctgg ccgctatgct gatccgagag ctgcgggcag ccctggaact
     6001 gttcgagcag gaaggcctgg ctccatatct gtcacggtgg gagaagctgg ataacttcat
     6061 caatagaccc gtgaagctga tcattgggga caaagagatt ttcgggatta gccgggggat
     6121 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
     6181 agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct
     6241 gggcctggac agcaccacaa gtttgtacaa aaaagcaggc tccaccatgg agaagtatgt
     6301 tagactacag aagattggag aaggttcatt tggaaaagcc attcttgtta aatctacaga
     6361 agatggcaga cagtatgtta tcaaggaaat taacatctca agaatgtcca gtaaagaaag
     6421 agaagaatca aggagagaag ttgcagtatt ggcaaacatg aagcatccaa atattgtcca
     6481 gtatagagaa tcatttgaag aaaatggctc tctctacata gtaatggatt actgtgaggg
     6541 aggggatctg tttaagcgaa taaatgctca gaaaggcgtt ttgtttcaag aggatcagat
     6601 tttggactgg tttgtacaga tatgtttggc cctgaaacat gtacatgata gaaaaattct
     6661 tcatcgagac attaaatctc agaacatatt tttaactaaa gatggaacag tacaacttgg
     6721 agattttgga attgctagag ttcttaatag tactgtagag ctggctcgaa cttgcatagg
     6781 gaccccatac tacttgtcac ctgaaatctg tgaaaacaaa ccttacaata ataaaagtga
     6841 catttgggct ctggggtgtg tcctttatga gctgtgtaca cttaaacatg cttttgaagc
     6901 tggcagtatg aaaaacctgg tactgaagat aatatctgga tcttttccac ctgtgtcttt
     6961 gcattattcc tatgatctcc gcagtttggt gtctcagtta tttaaaagaa atcctaggga
     7021 tagaccatca gtcaactcca tattggagaa aggttttata gccaaacgca ttgaaaagtt
     7081 tctctctcct cagcttattg cagaagaatt ttgtctaaaa acattttcga agtttggatc
     7141 acagcctata ccagctaaaa gaccagcttc aggacaaaac tcgatttctg ttatgcctgc
     7201 tcagaaaatt acaaagcctg ccgctaaata tggaatacct ttagcatata agaaatatgg
     7261 agataaaaaa ttacacgaaa agaaaccact gcaaaaacat aaacaggccc atcaaactcc
     7321 agagaagaga gtgaatactg gagaagaaag gaggaaaata tctgaggaag cagcaagaaa
     7381 gagaaggctg gaatttattg aaaaagaaaa gaaacaaaag gatcagatta ttagtttaat
     7441 gaaggctgaa caaatgaaaa ggcaagaaaa ggaaagggct ccttttctgg gcagtggagg
     7501 gactatagct ccatcatctt tttcttctcg aggacagtat gaacattacc atgccatttt
     7561 tgaccaaatg cagcaacaaa gagcagaaga taatgaagct aaatggaaaa gagaaatata
     7621 tggtcgaggt cttccagaaa ggcaaaaagg gcagctagct gtagaaagag ctaaacaagt
     7681 agaagagttc ctgcagcgaa aacgggaagc tatgcagaat aaagctcgag ccgaaggaca
     7741 tatggtttat ctggcaagac tgaggcaaat aagactacag aatttcaatg agcgccaaca
     7801 gattaaagcc aaacttcgtg gtgaaaagaa agaagctaat cattctgaag gacaagaagg
     7861 aagtgaagag gctgacatga ggcgcaaaaa aatcgaatca ctgaaggccc atgcaaatgc
     7921 acgtgctgct gtactaaaag aacaactaga acgaaagaga aaggaggctt atgagagaga
     7981 aaaaaaagtg tgggaagagc atttggtggc taaaggagtt aagagttctg atgtttctcc
     8041 acctttggga cagcatgaaa caggtggctc tccatcaaag caacagatga gatctgttat
     8101 ttctgtaact tcagctttga aagaagttgg cgtggacagt agtttaactg atacccggga
     8161 aacttcagaa gagatgcaaa agaccaacaa tgctatttca agtaagcgag aaatacttcg
     8221 tagattaaat gaaaatctta aagctcaaga agatgaaaaa ggaaagcaga atctctctga
     8281 tacttttgag ataaatgttc atgaagatgc caaagagcat gaaaaagaaa aatcagtttc
     8341 atctgatcgc aagaagtggg aggcaggagg tcaacttgtg attcctctgg atgagttaac
     8401 actagataca tccttctcta caactgaaag acatacagtg ggagaagtta ttaaattagg
     8461 tcctaatgga tctccaagaa gagcctgggg gaaaagtccg acagattctg ttctaaagat
     8521 acttggagaa gctgaactac aacttcagac agaactatta gaaaatacaa ctattagaag
     8581 tgagatttct cccgaagggg aaaagtacaa acccttaatt actggagaaa aaaaagtaca
     8641 atgtatttca catgaaataa acccatcagc tattgttgat tctcctgttg agacaaaaag
     8701 tcccgagttc agtgaggcat ctccacagat gtcattgaaa ctggaaggaa atttagaaga
     8761 acctgatgat ttggaaacag aaattctaca agagccaagt ggaacaaaca aagatgagag
     8821 cttgccatgc actattactg atgtgtggat tagtgaggaa aaagaaacaa aggaaactca
     8881 gtcggcagat aggatcacca ttcaggaaaa tgaagtttct gaagatggag tctcgagtac
     8941 tgtggaccaa cttagtgaca ttcatataga gcctggaacc aatgattctc agcactctaa
     9001 atgtgatgta gataagtctg tgcaaccgga accatttttc cataaggtgg ttcattctga
     9061 acacttgaac ttagtccctc aagttcaatc agttcagtgt tcaccagaag aatcctttgc
     9121 atttcgatct cactcgcatt taccaccaaa aaataaaaac aagaattcct tgctgattgg
     9181 actttcaact ggtctgtttg atgcaaacaa cccaaagatg ttaaggacat gttcacttcc
     9241 agatctctca aagctgttca gaacccttat ggatgttccc accgtaggag atgttcgtca
     9301 agacaatctt gaaatagatg aaattgaaga tgaaaacatt aaagaaggac cttctgattc
     9361 tgaagacatt gtgtttgaag aaactgacac agatttacaa gagctgcagg cctcgatgga
     9421 acagttactt agggaacaac ctggtgaaga atacagtgaa gaagaagagt cagtcttgaa
     9481 gaacagtgat gtggagccaa ctgcaaatgg gacagatgtg gcagatgaag atgacaatcc
     9541 cagcagtgaa agtgccctga acgaagaatg gcactcagat aacagtgatg gtgaaattgc
     9601 tagtgaatgt gaatgcgata gtgtctttaa ccatttagag gaactgagac ttcatctgga
     9661 gcaggaaatg ggctttgaaa aattctttga ggtttatgag aaaataaagg ctattcatga
     9721 agatgaagat gaaaatattg aaatttgttc aaaaatagtt caaaatattt tgggaaatga
     9781 acatcagcat ctttatgcca agattcttca tttagtcatg gcagatggag cctaccaaga
     9841 agataatgat gaataggacc cagctttctt gtacaaagtg gtgatatcca gcacagtggc
     9901 ggccgctcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     9961 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
    10021 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
    10081 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
    10141 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
    10201 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
    10261 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
    10321 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
    10381 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
    10441 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
    10501 ctggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
    10561 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
    10621 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
    10681 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
    10741 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
    10801 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
    10861 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
    10921 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
    10981 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg
    11041 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
    11101 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
    11161 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
    11221 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
    11281 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
    11341 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
    11401 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
    11461 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
    11521 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
    11581 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
    11641 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatct cgagggcccg
    11701 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
    11761 aaggggggac tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt
    11821 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    11881 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    11941 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    12001 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
    12061 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    12121 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    12181 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
    12241 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
    12301 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    12361 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
    12421 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
    12481 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
    12541 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
    12601 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
    12661 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
    12721 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    12781 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    12841 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    12901 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    12961 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
    13021 aacgcttaca attt
//
