LOCUS       sequence-374463-       10954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express APOE E4 allele from Ubiquitin C promoter, co-expressing 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10954)
  TITLE     Hart Lab APP and APOE Plasmids 2022
REFERENCE   2  (bases 1 to 10954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUGW-APOE-E4-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..10954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             2193..2246
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2259..2279
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2280..2990
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGREFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2425..2443)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2693..2712
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2887..2910
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3025..3613
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3078..3098)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3496..3507)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3616..3632)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3617..3633)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4138..4318
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4344..4361)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4350..4574
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4620..5048
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4707..4726)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4917..4938
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5057..5077)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5062..5391
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5242..5377
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5304..5323
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5439..5486
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5505..5879
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6009..6130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6046..6065)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6100..6119
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6179..6195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6179..6195)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6192..6214)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6203..6219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6227..6233,6234..6251,6252..6257))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6272..6293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6410..6427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6581..7169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6661..6680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7340..8131,8132..8200))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7963..7982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8201..8305)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8380..8399)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8571..8950
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8952..9150
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9105..9125
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9168..9348
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9395..9520
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10013..10246
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10431..10475
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10773..10890
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactaccac catgaaggtt ctgtgggctg
     1261 cgttgctggt cacattcctg gcaggatgcc aggccaaggt ggagcaagcg gtggagacag
     1321 agccggagcc cgagctgcgc cagcagaccg agtggcagag cggccagcgc tgggaactgg
     1381 cactgggtcg cttttgggat tacctgcgct gggtgcagac actgtctgag caggtgcagg
     1441 aggagctgct cagctcccag gtcacccagg aactgagggc gctgatggac gagaccatga
     1501 aggagttgaa ggcctacaaa tcggaactgg aggaacaact gaccccggtg gcggaggaga
     1561 cgcgggcacg gctgtccaag gagctgcagg cggcgcaggc ccggctgggc gcggacatgg
     1621 aggacgtgcg cggccgcctg gtgcagtacc gcggcgaggt gcaggccatg ctcggccaga
     1681 gcaccgagga gctgcgggtg cgcctcgcct cccacctgcg caagctgcgt aagcggctcc
     1741 tccgcgatgc cgatgacctg cagaagcgcc tggcagtgta ccaggccggg gcccgcgagg
     1801 gcgccgagcg cggcctcagc gccatccgcg agcgcctggg gcccctggtg gaacagggcc
     1861 gcgtgcgggc cgccactgtg ggctccctgg ccggccagcc gctacaggag cgggcccagg
     1921 cctggggcga gcggctgcgc gcgcggatgg aggagatggg cagccggacc cgcgaccgcc
     1981 tggacgaggt gaaggagcag gtggcggagg tgcgcgccaa gctggaggag caggcccagc
     2041 agatacgcct gcaggccgag gccttccagg cccgcctcaa gagctggttc gagcccctgg
     2101 tggaagacat gcagcgccag tgggccgggc tggtggagaa ggtgcaggct gccgtgggca
     2161 ccagcgccgc ccctgtgccc agcgacaatc acgagggcag gggaagtctt ctaacatgcg
     2221 gggacgtgga ggaaaatccc ggccctctag agatggctcc aaagaagaag cgtaaggtaa
     2281 tggtgagcaa gggcgaggag gacaacatgg ccatcatcaa ggagttcatg cgcttcaagg
     2341 tgcacatgga gggctccgtg aacggccgcg agttcgagat cgagggcgag ggcgagggcc
     2401 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     2461 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     2521 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     2581 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     2641 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     2701 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     2761 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgccg
     2821 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     2881 acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
     2941 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa attaagctag
     3001 taattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     3061 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3121 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3181 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3241 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3301 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3361 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3421 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3481 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3541 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3601 gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca
     3661 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     3721 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     3781 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     3841 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     3901 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     3961 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     4021 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     4081 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     4141 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     4201 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     4261 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     4321 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4381 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4441 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4501 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4561 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4621 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4681 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4741 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4801 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4861 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4921 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4981 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5041 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5101 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5161 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5221 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5281 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     5341 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     5401 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     5461 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     5521 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     5581 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     5641 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     5701 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     5761 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     5821 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     5881 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5941 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     6001 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6061 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6121 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6181 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6241 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6301 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6361 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6421 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6481 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6541 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6601 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6661 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6721 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6781 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6841 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6901 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6961 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7021 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7081 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7141 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7201 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7261 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7321 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7381 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7441 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7501 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7561 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7621 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7681 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7741 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7801 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7861 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7921 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7981 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8041 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8101 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8161 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8221 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8281 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     8341 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     8401 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     8461 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     8521 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     8581 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     8641 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     8701 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     8761 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     8821 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     8881 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     8941 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     9001 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     9061 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     9121 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
     9181 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9241 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9301 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     9361 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     9421 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     9481 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     9541 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     9601 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     9661 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     9721 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     9781 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     9841 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     9901 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     9961 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
    10021 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
    10081 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
    10141 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
    10201 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
    10261 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
    10321 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
    10381 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
    10441 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
    10501 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    10561 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
    10621 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
    10681 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
    10741 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
    10801 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
    10861 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
    10921 gtttggttaa ttaacccgtg tcggctccag atct
//
