LOCUS       sequence-374465-        8249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the mouse BDNF tagged with the FP mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8249)
  TITLE     Neurotrophin related plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-DBNF-mCherry
FEATURES             Location/Qualifiers
     source          1..8249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(101..120)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      914..1502
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1403..1422
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1656..1673
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1790..1811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1826..1831,1832..1849,1850..1856)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1864..1880
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1869..1891
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1888..1904
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1888..1904
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1923..1943
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1925..1943
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1971..2197
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2198..2378
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2425..2550
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3043..3276
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3461..3505
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3772..3888
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3911..4214
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4215..4418
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4369..4389
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4415..4439
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    6063..6651
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6116..6136)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6534..6545)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6738..6971
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7043..7164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7080..7099)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7134..7153
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7204..7339
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7359..7378)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7360..7378)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7388..7405)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7388..7404)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7397..7419)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7546..8001
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7633..7652)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7843..7864
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8027..8131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8132..8200,8201..8249,1..743)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
       61 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      121 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      181 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
      241 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
      301 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
      361 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
      421 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
      481 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
      541 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
      601 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
      661 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
      721 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
      781 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
      841 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
      901 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      961 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1021 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1081 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1141 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1201 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1261 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1321 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1381 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1441 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1501 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1561 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1621 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1681 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     1741 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     1801 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     1861 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     1921 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     1981 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     2041 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     2101 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     2161 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     2221 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     2281 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     2341 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     2401 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     2461 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     2521 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     2581 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     2641 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     2701 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     2761 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     2821 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     2881 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     2941 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     3001 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     3061 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     3121 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     3181 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     3241 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     3301 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     3361 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     3421 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     3481 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     3541 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     3601 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     3661 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     3721 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     3781 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     3841 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     3901 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     3961 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     4021 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     4081 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     4141 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     4201 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     4261 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     4321 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     4381 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     4441 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgcccg ggcgtacggg
     4501 atcccaccgg tgctcgagat gacaatcctg ttcctgacca tggtcatcag ctacttcggc
     4561 tgcatgaagg ccgctcccat gaaggaggtg aacgtccacg gccagggcaa cctggcctac
     4621 cctggcgtgc ggacccacgg caccctggaa agcgtgaacg gccccagagc tggcagcaga
     4681 ggcctgacca caaccagcct ggccgacacc ttcgagcacg tgatcgagga actgctggac
     4741 gaggaccaga aagtgcggcc caacgaggaa aaccacaagg acgccgacct gtacaccagc
     4801 agagtgatgc tgagcagcca ggtgcccctg gaaccccccc tgctgttcct gctggaagag
     4861 tacaagaact acctggacgc cgccaacatg agcatgagag tgcggagaca cagcgacccc
     4921 gccagacggg gcgagctgag cgtgtgtgac agcatcagcg agtgggtcac agccgccgac
     4981 aagaaaaccg ccgtggacat gagcggcggc accgtgaccg tgctggaaaa ggtgcccgtg
     5041 tccaagggcc agctgaagca gtacttctac gagacaaagt gcaaccccat gggctacacc
     5101 aaagagggct gcagaggcat cgacaagaga cactggaaca gccagtgcag aaccacccag
     5161 agctacgtgc gggccctgac aatggacagc aagaagagaa tcggctggcg gttcatcaga
     5221 atcgacacca gctgcgtgtg caccctgacc atcaagagag gcagatccgg cggctctggc
     5281 ggcaccggcg gaatggtgtc taagggcgaa gaggacaaca tggccatcat caaagaattc
     5341 atgcggttca aggtgcacat ggaaggctct gtgaacggcc acgagttcga gatcgagggc
     5401 gagggggagg gcagacccta cgagggcaca cagaccgcca agctgaaagt gaccaagggc
     5461 ggacccctgc ccttcgcctg ggacatcctg agcccccagt ttatgtacgg cagcaaggcc
     5521 tacgtgaagc accccgccga catccccgac tacctgaagc tgagcttccc cgagggcttc
     5581 aagtgggaga gagtgatgaa cttcgaggac ggcggcgtgg tcacagtgac ccaggacagc
     5641 agcctgcagg acggcgagtt catctacaaa gtgaagctga gaggcaccaa cttccccagc
     5701 gacggccccg tgatgcagaa aaagaccatg ggctgggagg ccagcagcga gagaatgtac
     5761 cctgaggacg gcgccctgaa gggcgagatc aagcagagac tgaagctgaa ggatggcggc
     5821 cactacgacg ccgaagtgaa aaccacctac aaggccaaga aacccgtgca gctgcctggc
     5881 gcctacaacg tgaacatcaa gctggacatc accagccaca acgaggacta caccatcgtg
     5941 gaacagtacg agagagccga gggcagacac tccaccggcg gcatggacga gctgtacaaa
     6001 tgagctagct ctagacctgc agggtcgact ccggatgatc agggccctgt acagatatcg
     6061 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6121 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6181 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6241 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6301 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6361 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6421 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     6481 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6541 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6601 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     6661 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6721 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     6781 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6841 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6901 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6961 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7021 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7081 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7141 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     7201 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7261 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7321 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     7381 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     7441 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     7501 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     7561 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7621 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7681 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7741 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7801 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7861 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7921 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7981 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8041 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8101 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8161 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8221 agtaaaagat gctgaagatc agttgggtg
//
