LOCUS       sequence-374466-        8276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the mouse NT-3 tagged with the FP mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8276)
  TITLE     Neurotrophin related plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-NT-3-mCherry
FEATURES             Location/Qualifiers
     source          1..8276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(98..117)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      911..1499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1400..1419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1653..1670
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1787..1808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1823..1828,1829..1846,1847..1853)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1861..1877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1866..1888
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1885..1901
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1885..1901
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1920..1940
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1922..1940
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1968..2194
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2195..2375
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2422..2547
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3040..3273
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3458..3502
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3769..3885
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3908..4211
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4212..4415
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4366..4386
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4412..4436
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    6087..6675
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6140..6160)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6558..6569)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6762..6995
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7067..7188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7104..7123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7158..7177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7228..7363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7383..7402)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7384..7402)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7412..7429)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7412..7428)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7421..7443)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7570..8025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7657..7676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7867..7888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8051..8155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8156..8224,8225..8276,1..740)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
       61 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      121 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      181 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      241 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      301 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      361 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      421 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      481 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      541 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
      601 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
      661 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
      721 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
      781 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
      841 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      901 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      961 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1021 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1081 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1141 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1201 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1261 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1321 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1381 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1441 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1501 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1561 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     1621 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     1681 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     1741 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     1801 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     1861 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     1921 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     1981 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     2041 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     2101 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     2161 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     2221 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     2281 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     2341 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     2401 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     2461 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     2521 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     2581 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     2641 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     2701 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     2761 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     2821 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     2881 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     2941 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     3001 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     3061 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     3121 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     3181 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     3241 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     3301 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     3361 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     3421 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     3481 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     3541 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     3601 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     3661 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     3721 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
     3781 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     3841 acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga taagcttggg
     3901 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     3961 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4021 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4081 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4141 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4201 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     4261 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     4321 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     4381 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     4441 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgcccgggc gtacgggatc
     4501 ccaccggtgc tcgagatgag catcctgttc tacgtgatct tcctggccta cctgagaggc
     4561 atccagggca acagcatgga ccagagaagc ctgcccgagg acagcctgaa ctccctgatc
     4621 atcaagctga tccaggccga catcctgaag aacaagctga gcaagcagat ggtggacgtg
     4681 aaagagaact accagagcac cctgcccaag gccgaggccc ccagagagcc tgagcagggc
     4741 gaggccacca gatccgagtt ccagcccatg atcgccaccg acaccgagct gctgagacag
     4801 cagagaagat acaacagccc cagagtgctg ctgagcgaca gcacccccct ggaacccccc
     4861 cctctgtacc tgatggaaga ttacgtgggc aaccccgtgg tggccaacag aaccagcccc
     4921 agaagaaaga gatacgccga gcacaagagc cacagaggcg agtacagcgt gtgcgacagc
     4981 gagagcctgt gggtcacaga caagagcagc gccatcgaca tcaggggcca ccaggtcacc
     5041 gtgctgggcg agatcaagac cggcaacagc cccgtgaagc agtacttcta cgagacaaga
     5101 tgcaaagagg ccagacccgt caagaacggc tgcagaggca tcgacgacaa gcactggaac
     5161 agccagtgca agaccagcca gacctacgtg cgggccctga ccagcgagaa caacaagctc
     5221 gtcggatggc ggtggatcag aatcgacacc agctgcgtgt gcgccctgag cagaaagatc
     5281 ggcagaacca gcggcggctc tggcggcaca ggcggcatgg tgtctaaggg cgaagaggac
     5341 aacatggcca tcatcaaaga attcatgcgg ttcaaggtgc acatggaagg cagcgtgaac
     5401 ggccacgagt tcgagatcga gggcgagggg gagggcagac cctacgaggg cacacagacc
     5461 gccaagctga aagtgaccaa gggcggaccc ctgcccttcg cctgggacat cctgagcccc
     5521 cagtttatgt acggcagcaa ggcctacgtg aagcaccccg ccgacatccc cgactacctg
     5581 aagctgagct tccccgaggg cttcaagtgg gagagagtga tgaacttcga ggacggcggc
     5641 gtcgtcacag tgacccagga cagcagcctg caggacggcg agttcatcta caaagtgaag
     5701 ctgagaggca ccaacttccc cagcgacggc cccgtgatgc agaaaaagac catgggctgg
     5761 gaggccagca gcgagagaat gtaccctgag gacggcgccc tgaaggggga gatcaagcag
     5821 agactgaagc tgaaggatgg cggccactac gacgccgaag tgaaaaccac ctacaaggcc
     5881 aagaaacccg tgcagctgcc tggcgcctac aacgtgaaca tcaagctgga catcaccagc
     5941 cacaacgagg actacaccat cgtggaacag tacgagagag ccgagggcag acactccacc
     6001 ggcggcatgg acgagctgta caaatgagct agctctagac ctgcagggtc gactccggat
     6061 gatcagggcc ctgtacagat atcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     6121 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6181 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6241 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6301 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6361 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6421 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6481 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6541 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6601 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6661 gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc
     6721 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6781 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     6841 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6901 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6961 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     7021 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     7081 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7141 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     7201 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7261 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7321 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     7381 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     7441 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     7501 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     7561 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7621 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     7681 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7741 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     7801 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7861 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     7921 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7981 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     8041 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8101 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8161 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8221 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcac
//
