LOCUS       sequence-374467-        8949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express a Human TrkC receptor that binds only BDNF. The second 
            IgG like domain which is the main binding domain comes from the TrkB
            receptor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8949)
  AUTHORS   Urfer R, Tsoulfas P, O'Connell L, Hongo JA, Zhao W, Presta LG
  TITLE     High resolution mapping of the binding site of TrkA for nerve growth
            factor and TrkC for neurotrophin-3 on the second immunoglobulin-like
            domain of the Trk receptors.
  JOURNAL   J Biol Chem. 1998 Mar 6;273(10):5829-40. doi: 
            10.1074/jbc.273.10.5829.
  PUBMED    9488719
REFERENCE   2  (bases 1 to 8949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.273.10"; journalName: "J Biol Chem"; date: "1998-03-6- 
            6"; volume: "273"; issue: "10"; pages: "5829-40"
COMMENT     Sequence Label: pMEXneo-TrkC-TrkB-hybrid
FEATURES             Location/Qualifiers
     source          1..8949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    25..159
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(49..68)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     103..122
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(584..604)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          734..799
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1219..2013)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1330..1349)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1940..1959
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2370..2699)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2384..2519
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2684..2704
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(2966..2982)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2966..2982)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2979..3001)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2990..3006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3014..3020,3021..3038,3039..3044))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3059..3080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3197..3214)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3368..3956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3448..3467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4127..4918,4919..4987))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4750..4769
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4988..5092)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5160..5178
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     5551..5573
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5565..5582
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5566..5582
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggtggctgg tggtcatgaa ttccgatcca gacatgataa gatacattga tgagtttgga
       61 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
      121 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
      181 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
      241 aaatgtggta tggctgatta tgatctctag tcaaggcact atacatcaaa tattccttat
      301 taaccccttt acaaattaaa aagctaaagg tacacaattt ttgagcatag ttattaatag
      361 cagacactct atgcctgtgt ggagtaagaa aaaacagtat gttatgatta taactgttat
      421 gcctacttat aaaggttaca gaatattttt ccataatttt cttgtatagc agtgcagctt
      481 tttcctttgt ggtgtaaata gcaaagcaag caagagttct attactaaac acagcatgac
      541 tcaaaaaact tagcaattct gaaggaaagt ccttggggtc ttctaccttt ctcttctttt
      601 ttggaggagt agaatgttga gagtcagcag tagcctcatc atcactagat ggcatttctt
      661 ctgagcaaaa caggttttcc tcattaaagg cattccacca ctgctcccat tcatcagttc
      721 cataggttgg aatctaaaat acacaaacaa ttagaatcag tagtttaaca cattatacac
      781 ttaaaaattt tatatttacc ttagagcttt aaatctctgt aggtagtttg tccaattatg
      841 tcacaccaca gaagtaaggt tccttcacaa agatccggac caaagcggcc atcgtgcctc
      901 cccactcctg cagttcgggg gcatggatgc gcggatagcc gctgctggtt tcctggatgc
      961 cgacggattt gcactgccgg tagaactccg cgaggtcgtc cagcctcagg cagcagctga
     1021 accaactcgc gaggggatcg agcccggggt gggcgaagaa ctccagcatg agatccccgc
     1081 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
     1141 aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt
     1201 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     1261 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     1321 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     1381 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     1441 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     1501 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     1561 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     1621 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     1681 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     1741 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     1801 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
     1861 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     1921 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     1981 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     2041 atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     2101 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     2161 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     2221 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     2281 tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg
     2341 ccctgagtgc ttgcggcagc gtgaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct
     2401 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     2461 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     2521 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
     2581 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
     2641 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     2701 ttccacagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2761 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2821 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     2881 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2941 cggatcggaa ttaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
     3001 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3061 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3121 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3181 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3241 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3301 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3361 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3421 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3481 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3541 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3601 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3661 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3721 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3781 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     3841 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3901 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3961 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4021 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4081 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4141 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4201 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4261 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4321 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4381 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4441 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4501 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4561 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4621 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4681 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4741 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4801 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4861 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4921 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4981 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5041 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5101 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     5161 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     5221 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     5281 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     5341 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     5401 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     5461 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     5521 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     5581 gtgccaagct aattctctgt actctatatt taagtttcct ataaatctat agcttttcta
     5641 aaaataaaat ctatttaaaa attacttgaa tgaaaggtaa acacgcctct ttcacacagt
     5701 ctgatgcaaa gtctcatcaa gaattgggca gtcctgggaa gttggaaccc tgtaaatacg
     5761 gactccaccg tgcagacaat gccatgtaga gaagcgtgtt cccaagatcc cagcttcccc
     5821 actctgggtc cttcccctca tagatataaa cgtgaaagac cccagccgta ggtttggcaa
     5881 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa
     5941 gttcagatca aggtcaggaa cagagagaca gctgaatatg ggccaaacag gatatctgtg
     6001 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga attggggcaa
     6061 acaggatatc tgcggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
     6121 agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
     6181 aggacctgaa aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     6241 tgttcgcgcg cttccgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc
     6301 gccagtcttc cgatagactg cgtcgcccag cttgcatgcc tgcaggtcga ctctagagga
     6361 tccgcgtcgg agatggatgt ctctctttgc ccagccaagt gtagtttctg gcggattttc
     6421 ttgctgggaa gcgtctggct ggactatgtg ggctccgtgc tggcttgccc tgcaaattgt
     6481 gtctgcagca agactgagat caattgccgg cggccggacg atgggaacct cttccccctc
     6541 ctggaagggc aggattcagg gaacagcaat gggaacgcca atatcaacat cacggacatc
     6601 tcaaggaata tcacttccat acgcatagag aactggcgca gtcttcacac gctcaacgcc
     6661 gtggacatgg agctctacac cggacttcaa aagctgacca tcaagaactc aggacttcgg
     6721 agcattcagc ccagagcctt tgccaagaac ccccatttgc gttatataaa cctgtcaagt
     6781 aaccggctca ccacactctc gtggcagctc ttccagacgc tgagtcttcg ggaattgcag
     6841 ttggagcaga actttttcaa ctgcagctgt gacatccgct ggatgcagct ctggcaggag
     6901 cagggggagg ccaagctcaa cagccagaac ctctactgca tcaatgctga tggctcccag
     6961 cttcctctct tccgcatgaa catcagtcag tgtgaccttc ctgagatcag cgtgagccac
     7021 gtcaacctga ccgtacgaga gggtgacaat gctgttatca cttgcaatgg ctctggatca
     7081 ccccttcctg atgtggactg gatagtcact gggctgcagt ccatcaacac tcaccagacc
     7141 aatctgaact ggaccaatgt tcatgccatc aacttgacgc tggtgaatgt gacgagtgag
     7201 gacaatggct tcaccctgac gtgcattgca gagaacgtgg tgggcatgag caacgcgtct
     7261 gtcaacctca ctgtgcattt tgcaccaact atcacatttc tcgaatctcc aacctcagac
     7321 caccactggt gcattccatt cactgtgaaa ggcaacccaa aaccagcgct tcagtggttc
     7381 tataacgggg caatattgaa tgagtccaaa tacatctgta ctaaaataca tgttaccaat
     7441 cacacggagt accacggctg cctccagctg gataatccca ctcacatgaa caatggggac
     7501 tacactctaa tagccaagaa tgagtatggg aaggatgaga aacagatttc tgctcacttc
     7561 atgggctggc ctggaattga cgatggtgca aacccaaatt atcctgatgt aatttatgaa
     7621 gattatggaa ctgcagcgaa tgacatcggg gacaccacga acagaagtaa tgaaatccct
     7681 tccacagacg tcacccacaa accagaagaa gacacttttg gggtatccat agcagttgga
     7741 cttgctgctt ttgcctgtgt cctgttggtg gttctcttcg tcatgatcaa caaatatggt
     7801 cgacggtcca aatttggaat gaagggtccc gtggctgtca tcagtggtga ggaggactca
     7861 gccagcccac tgcaccacat caaccacggc atcaccacgc cctcgtcact ggatgccggg
     7921 cccgacactg tggtcattgg catgactcgc atccctgtca ttgagaaccc ccagtacttc
     7981 cgtcagggac acaactgcca caagccggac acgtatgtgc agcacattaa gaggagagac
     8041 atcgtgctga agcgagaact gggtgaggga gcctttggaa aggtcttcct ggccgagtgc
     8101 tacaacctca gcccgaccaa ggacaagatg cttgtggctg tgaaggccct gaaggatccc
     8161 accctggctg cccggaagga tttccagagg gaggccgagc tgctcaccaa cctgcagcat
     8221 gagcacattg tcaagttcta tggagtgtgc ggcgatgggg accccctcat catggtcttt
     8281 gaatacatga agcatggaga cctgaataag ttcctcaggg cccatgggcc agatgcaatg
     8341 atccttgtgg atggacagcc acgccaggcc aagggtgagc tggggctctc ccaaatgctc
     8401 cacattgcca gtcagatcgc ctcgggtatg gtgtacctgg cctcccagca ctttgtgcac
     8461 cgagacctgg ccaccaggaa ctgcctggtt ggagcgaatc tgctagtgaa gattggggac
     8521 ttcggcatgt ccagagatgt ctacagcacg gattattaca ggctctttaa tccatctgga
     8581 aatgattttt gtatatggtg tgaggtggga ggacacacca tgctccccat tcgctggatg
     8641 cctcctgaaa gcatcatgta ccggaagttc actacagaga gtgatgtatg gagcttcggg
     8701 gtgatcctct gggagatctt cacctatgga aagcagccat ggttccaact ctcaaacacg
     8761 gaggtcattg agtgcattac ccaaggtcgt gttttggagc ggccccgagt ctgccccaaa
     8821 gaggtgtacg atgtcatgct ggggtgctgg cagagggaac cacagcagcg gttgaacatc
     8881 aaggagatct acaaaatcct ccatgctttg gggaaggcca ccccaatcta cctggacatt
     8941 cttggctag
//
