LOCUS       sequence-37448-e        1731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3545 (=HL 770 + pHL 1213)
FEATURES             Location/Qualifiers
     source          1..1731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(15..34)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(611..705)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(735..1526,1527..1595))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1358..1377
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1596..1700)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
       61 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      121 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      181 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      241 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      301 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      361 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      421 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      481 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      541 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgac
      601 tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
      661 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgtaa
      721 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      781 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
      841 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
      901 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
      961 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1021 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1081 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1141 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1201 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1261 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1321 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1381 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1441 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1501 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1561 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1621 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1681 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
