LOCUS       sequence-374484-       13371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13371)
  AUTHORS   Loftus SK, Larson DM, Watkins-Chow D, Church DM, Pavan WJ
  TITLE     Generation of RCAS vectors useful for functional genomic analyses.
  JOURNAL   DNA Res. 2001 Oct 31. 8(5):221-6.
  PUBMED    11759842
REFERENCE   2  (bases 1 to 13371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res. 
            2001 Oct 31. 8(5):221-6."
COMMENT     Sequence Label: RCASBP-Y DV
FEATURES             Location/Qualifiers
     source          1..13371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..262
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     protein_bind    7275..7399
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7424..7429,7430..7447,7448..7454)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7508..8167
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7574..7593)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8509..8814
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8693..8712
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8855..8979)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        9141..9402
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(9992..10014)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10003..10019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10027..10033,10034..10051,10052..10057))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(10100..10118)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10186..10290
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10291..10359,10360..11151)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10509..10528)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11322..11910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11811..11830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12064..12081
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12096..12236
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12162..12181)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12322..12344
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(12338..12529)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 ccgcattgca gagatattgt atttaagtgc ctagctcgat acaataaacg ccatttgacc
      241 attcaccaca ttggtgtgca cctgggttga tggccggacc gttgattccc tgacgactac
      301 gagcacctgc atgaagcaga aggcttcatt tggtgacccc gacgtgatag ttagggaata
      361 gtggtcggcc acagacggcg tggcgatcct gtctccatcc gtctcgtcta tcgggaggcg
      421 agttcgatga ccctggtgga gggggctgcg gcttagggag gcagaagctg agtaccgtcg
      481 gagggagctc cagggcccgg agcgactgac ccctgccgag aactcagagg gtcgtcggaa
      541 gacggagagt gagcccgacg accaccccag gcacgtcttt ggtcggcctg cggatcaagc
      601 atggaagccg tcattaaggt gatttcgtcc gcgtgtaaaa cctattgcgg gaaaatctct
      661 ccttctaaga aggaaatagg ggccatgttg tccctgttac aaaaggaagg gttgcttatg
      721 tctccctcag atttatattc tccggggtcc tgggatccca tcactgcggc gctctcccag
      781 cgggcaatgg tacttggaaa atcgggagag ttaaaaacct ggggattggt tttgggggca
      841 ttgaaggcgg ctcgagagga acaggttaca tctgagcaag caaagttttg gttgggatta
      901 gggggaggga gggtctctcc cccaggtccg gagtgcatcg agaaaccagc tacggagcgg
      961 cgaatcgaca aaggggagga ggtgggagaa acaactgtgc agcgagatgc gaagatggcg
     1021 ccagaggaag cggccacacc taaaaccgtt ggcacatcct gctatcattg cggaacagct
     1081 gttggctgca attgcgccac cgccacagcc tcggcccctc ctccccctta tgtggggagt
     1141 ggtttgtatc cttccctggc gggggtggga gagcagcagg gccagggaga taacacgtct
     1201 cggggggcgg agcagccaag ggaggagcca gggcacgcgg gtcaggcccc tgggccggcc
     1261 ctgactgact gggcaagggt aagggaggag cttgcgagta ctggtccgcc cgtggtggcc
     1321 atgcctgtag tgattaagac agagggaccc gcctggaccc ctctggagcc aaaattgatc
     1381 acaagactgg ctgatacggt caggaccaag ggcttacgat ccccgatcac tatggcagaa
     1441 gtggaagcgc tcatgtcctc cccgttgctg ccgcatgacg tcacgaatct aatgagagtg
     1501 attttaggac ctgccccata tgccttatgg atggacgctt ggggagtcca actccagacg
     1561 gttatagcgg cagccactcg cgacccccga cacccagcga acggtcaagg gcggggggaa
     1621 cggactaact tggatcgatt aaagggctta gctgatggga tggtgggcaa cccacagggt
     1681 caggccgcat tattaagacc gggggaattg gttgctatta cggcgtcggc tctccaggcg
     1741 tttagagaag ttgcccggct ggcggaacct gcaggtccat gggcggacat cacgcaggga
     1801 ccatctgagt cctttgttga ttttgccaat cggcttataa aggcggttga ggggtcagat
     1861 ctcccgcctt ccgcgcgggc tccggtgatc attgactgct ttaggcagaa gtcacagcca
     1921 gatattcagc agcttatacg ggcagcaccc tccacgctga ccaccccagg agagataatc
     1981 aaatatgtgc tagacaggca gaagattgcc cctcttacgg atcaaggcat agccgcggcc
     2041 atgtcgtctg ctatccagcc cttagttatg gcagtagtca atagagagag ggatggacaa
     2101 actgggtcgg gtggtcgtgc ccgagggctc tgctacactt gtggatcccc gggacattat
     2161 caggcacagt gcccgaaaaa acgaaagtca ggaaacagcc gtgagcgatg tcagctgtgt
     2221 gacgggatgg gacacaacgc taaacagtgt aggaagcggg atggcaacca gggccaacgc
     2281 ccaggaagag gtctctcttc ggggccgtgg cccggccctg agcagcctgc cgtctcgtta
     2341 gcgatgacaa tggaacataa agatcgcccc ttggttaggg tcattctgac taacactggg
     2401 agtcatccag tcaaacaacg ttcggtgtat atcaccgcgc tgttggactc cggagcggac
     2461 atcactatta tttcggagga ggattggcct actgattggc cggtggtgga caccgcgaac
     2521 ccacagatcc atggcatagg agggggaatt cccatgcgaa aatcccggga tatgatagag
     2581 gtgggggtta ttaaccgaga cgggtcgttg gagcgacccc tgctcctctt ccccgcagtc
     2641 gctatggtta gagggagtat cctaggaaga gattgtctgc agggcctagg gctccgcttg
     2701 acaaatttat agggagggcc actgttctta ctgttgcgct acatctggct attccgctca
     2761 aatggaagtc agaccgcacg cctgtgtgga ttgaccagtg gccccttcct gaaggtaaac
     2821 ttgtagcgct aacgcaatta gtggaaaaag aattacagtt aggacatata gagccctcac
     2881 ttagttgttg gaacacacct gtttttgtga tccggaaggc ttccgggtct tatcgcttat
     2941 tgcatgattt gcgcgctgtt aacgccaagc ttgttccttt tggggccgtc caacaggggg
     3001 cgccagttct ctccgcgctc ccgcgtggct ggcccctgat ggtcctagac ctcaaggatt
     3061 gcttcttttc tatccctctt gcggaacaag atcgcgaagc ttttgcattt acgctcccct
     3121 ctgtgaataa ccaggccccc gctcgaagat tccaatggaa ggtcttgccc caagggatga
     3181 cctgttctcc cactatctgt cagttggtag tgggtcaggt gctcgagccc ttgcgactca
     3241 agcacccagc tctgcgcatg ttgcattata tggacgatct tttgctagcc gcctcaagtc
     3301 atgatgggtt ggaagcggca gggaaggagg ttatcggtac attggaaaga gccgggttca
     3361 ctatttcgcc ggataagatc cagagggagc ccggagtaca atatcttggg tacaagttag
     3421 gcagtacgta tgtagcaccc gtaggcttgg tagcagaacc caggatagcc accttgtggg
     3481 atgttcaaaa gctggtgggg tcacttcagt ggcttcgccc agcgttaggg atcccgccac
     3541 gactgatggg tcccttttat gagcagttac gagggtcaga tcctaacgag gcgagggaat
     3601 ggaatctaga catgaaaatg gcctggagag agatcgtaca gcttagcact actgctgcct
     3661 tggaacgatg ggaccctgcc cagcctctgg aaggagcggt cgctagatgt gaacaggggg
     3721 caataggggt cctgggacag ggactgtcca cacacccaag gccatgtttg tggttattct
     3781 ccacccaacc caccaaggcg tttactgctt ggttagaagt gctcaccctt ttgattacta
     3841 agctacgcgc ttcggcagtg cgaacctttg gcaaggaggt tgatatcctc ctgttgcctg
     3901 catgcttccg ggaggacctt ccgctcccgg aggggatcct gttagcactt agggggtttg
     3961 caggaaaaat caggagtagt gacacgccat ctatttttga cattgcgcgt ccactgcatg
     4021 tttctctgaa agtgagggtt accgaccacc ctgtgccggg acccactgtc tttaccgacg
     4081 cctcctcaag cacccataaa ggggtggtag tctggaggga gggcccaagg tgggagataa
     4141 aagaaatagt tgatttgggg gcaagtgtac aacaactgga ggcacgcgct gtggccatgg
     4201 cacttctgct gtggccgaca acgcccacta atgtagtgac tgactctgcg tttgttgcga
     4261 aaatgttact caagatggga caggagggag tcccgtctac agcggcagct tttattttag
     4321 aggatgcgtt aagccaaagg tcagccatgg ccgccgttct ccacgtgcgg agtcattctg
     4381 aagtgccagg gtttttcaca gaaggaaatg acgtggcaga tagccaagcc acctttcaag
     4441 cgtatccctt gagagaggct aaagatcttc ataccgctct ccatattgga ccccgcgcgc
     4501 tatccaaagc gtgtaatata tctatgcagc aggctaggga ggttgttcag acctgcccgc
     4561 attgtaattc agcccctgcg ttggaggccg gggtaaaccc taggggtttg ggacccctac
     4621 agatatggca gacagacttt acgcttgagc ctagaatggc tccccgttcc tggctcgctg
     4681 ttactgtgga caccgcctca tcagcgatag tcgtaactca gcatggccgt gttacatcgg
     4741 ttgctgcaca acatcattgg gccacggcta tcgccgtttt gggaagacca aaggccataa
     4801 aaacagataa cgggtcctgc ttcacgtcta aatccacgcg ggagtggctc gcgagatggg
     4861 ggatagcaca caccaccggg attccgggaa attcccaggg tcaagctatg gtagagcggg
     4921 ccaaccggct cctgaaagat aagatccgtg tgcttgcgga aggggacggc tttatgaaaa
     4981 gaatccccgc cagcaaacag ggggaactac tagccaaagc aatgtatgcc ctcaatcact
     5041 ttgagcgtgg tgaaaacaca aaaacaccgg tacaaaaaca ctggagacct accgttctta
     5101 cagaaggacc cccggttaaa atacgaatag agacagggga gtgggaaaaa ggatggaacg
     5161 tgctggtctg gggacgaggt tatgccgctg tgaaaaacag ggacactgat aaggttattt
     5221 gggtaccctc tcggaaagtt aaaccggatg tcacccaaaa ggatgaggtg actaagaaag
     5281 atgaggcgag ccctcttttt gcaggcattt ctgactggat accctgggaa gacgagcaag
     5341 aaggactcca aggagaaacc gctagcaaca agcaagaaag acccggagaa gacacccttg
     5401 ctgccaacga gagttaatta tattctcatt attggtgtcc tggtcttgtg tgaggttacg
     5461 ggggtaagag ctgatgtcca cttactcgag cagccaggga acctttggat tacatgggcc
     5521 aaccgtacag gccaaacgga tttttgcctc tctacacagt cagccacctc cccttttcaa
     5581 acatgtttga taggtatccc gtcccctatt tccgagggtg attttaaggg atatgtttct
     5641 gatacaaatt gcaccacctt gggaactgat cggttagtct cgtcagccga ctttactggc
     5701 ggacctgaca acagtaccac cctcacttat cggaaggtct catgcttgtt gttaaagctg
     5761 aatgtctcta tgtgggatga gccacctgaa ctacagctgt taggttccca gtctctccct
     5821 aacattacta atattgctca gatttccggt ataaccgggg gatgcgtagg cttcagacca
     5881 caaggggttc cttggtatct aggttggtct agacaggagg ccacgcggtt tctccttaga
     5941 cacccctctt tctctaaatc cacggaaccg tttacggtgg tgacagcgga taggcacaat
     6001 ctttttatgg ggagtgagta ctgcggtgca tatggctaca gattttggaa catgtataac
     6061 tgctcacagg tggggcggca gtaccgctgt ggtaatgcgc gcagcccccg cccgggtctt
     6121 cctgaaatcc agtgtacaag gagaggaggc aaatgggtta atcaatcaca ggaaattaat
     6181 gagtcggagc cgttcagctt tacggtgaac tgtacagcta gtagtttggg taatgccagt
     6241 gggtgttgcg gaaaagcagg cacgattctc ccgggaaagt gggtcgacag cacacaaggt
     6301 agtttcacca aaccaaaagc gctaccaccc gcaattttcc tcatttgtgg ggatcgcgca
     6361 tggcaaggaa ttcccagtcg tccggtaggg ggcccctgct atttaggcaa gcttaccatg
     6421 ttagcaccta agcatacaga tattctcaag gtgcttgtca attcatcgcg gacaggtata
     6481 agacgtaaac gaagcacctc acacctggat gatacatgct cagatgaagt gcagctttgg
     6541 ggtcctacag caagaatctt tgcatctatc ctagccccgg gggtagcagc tgcgcaagcc
     6601 ttaagagaaa ttgagagact agcctgttgg tccgttaaac aggctaactt gacaacatca
     6661 ctcctcgggg acttattgga tgatgtcacg agtattcgac acgcggtcct gcagaaccga
     6721 gcggctattg acttcttgct cctagctcac ggccatggct gtgaggacgt tgccggaatg
     6781 tgctgtttca atttgagtga tcacagtgag tctatacaga agaagttcca gctaatgaag
     6841 gaacatgtca ataagatcgg cgtggacagc gacccaattg gaagttggct gcgaggacta
     6901 ttcgggggaa taggagaatg ggccgttcat ttgctgaaag gactgctttt ggggcttgta
     6961 gttattttgt tgctagtagt gtgcctgcct tgccttttgc aaatcgtatg cggtaacatc
     7021 agaaagatga ttaataactc catcagctac cacacggaat ataagaagct gcaaaaggcc
     7081 tgtgggcagc ctgaaagcag aatagtataa ggcagtacat gggtggtggt atagcgcttg
     7141 cgagtccatc gaggaaggca ggaaagacag ctattggtaa ttgtgaaata cgcttttgtc
     7201 tgtgtgctgc aggagctgag ctgactctgc tggtggcctc gcgtaccact gtggcatcgg
     7261 cggccgcgtt tatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa
     7321 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
     7381 aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc
     7441 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga
     7501 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
     7561 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
     7621 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     7681 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
     7741 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     7801 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     7861 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     7921 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     7981 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     8041 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
     8101 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     8161 ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
     8221 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
     8281 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
     8341 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
     8401 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
     8461 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag
     8521 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
     8581 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
     8641 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
     8701 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
     8761 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     8821 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt
     8881 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     8941 attttacgtt tctcgttcag ctttcttgta caaagtggtg ataaacttaa ttaaatttaa
     9001 atatcgatgc gatgtacggg ccagatatac gcgtatctga ggggactagg gtgtgtttag
     9061 gcgaaaagcg gggcttcggt tgtacgcggt taggagtccc ctcaggatat agtagtttcg
     9121 cttttgcata gggaggggga aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     9181 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     9241 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     9301 attggacgaa ccactgaatt ccgcattgca gagatattgt atttaagtgc ctagctcgat
     9361 acaataaacg ccatttgacc attcaccaca ttggtgtgca cctgggttga tggccggacc
     9421 gttgattccc tgacgactac gagcacctgc atgaagcaga aggcttcatt tggtgacccc
     9481 gacgtgatag ttagggaata gtggtcggcc acagacggcg tggcgatcct gtctccatcc
     9541 gtctcgtcta tcgggaggcg agttcgatga ccctggtgga gggggctgcg gcttagggag
     9601 gcagaagctg agtaccgtcg gaggggatcc acaggacggg tgtggtcgcc atgatcgcgt
     9661 agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg
     9721 acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca
     9781 cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta
     9841 ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga
     9901 gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac
     9961 taccgcatta aagctccaag cttggctgtt tcctgtgtga aattgttatc cgctcacaat
    10021 tccacacatt atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    10081 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
    10141 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
    10201 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
    10261 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
    10321 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
    10381 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
    10441 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
    10501 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
    10561 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
    10621 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
    10681 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
    10741 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
    10801 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
    10861 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
    10921 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
    10981 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    11041 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    11101 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    11161 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    11221 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    11281 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    11341 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    11401 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    11461 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    11521 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    11581 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    11641 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    11701 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    11761 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    11821 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    11881 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    11941 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    12001 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    12061 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
    12121 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
    12181 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
    12241 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
    12301 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
    12361 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
    12421 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
    12481 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
    12541 tgtaaggagg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
    12601 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
    12661 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
    12721 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
    12781 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
    12841 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
    12901 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
    12961 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
    13021 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
    13081 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
    13141 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
    13201 acgcaacgcg gggaggcaga caaggtatag ggcggcgatg cgatgtacgg gccagatata
    13261 cgcgtatctg aggggactag ggtgtgttta ggcgaaaagc ggggcttcgg ttgtacgcgg
    13321 ttaggagtcc cctcaggata tagtagtttc gcttttgcat agggaggggg a
//
