LOCUS       sequence-374497-       12232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Flag-LRRK2 K17A/K18A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12232)
  AUTHORS   Vides EG, Adhikari A, Chiang CY, Lis P, Purlyte E, Limouse C, 
            Shumate JL, Spinola-Lasso E, Dhekne HS, Alessi DR, Pfeffer SR
  TITLE     A feed-forward pathway drives LRRK2 kinase membrane recruitment and 
            activation.
  JOURNAL   Elife. 2022 Sep 23;11:e79771. doi: 10.7554/eLife.79771.
  PUBMED    36149401
REFERENCE   2  (bases 1 to 12232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-09-23"; volume: "11:e79771. doi"; pages: " 
            10.7554/eLife.79771"
COMMENT     Sequence Label: pCMV Flag-LRRK2 K17A/K18A
FEATURES             Location/Qualifiers
     source          1..12232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..126)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        111..440
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      291..426
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(478..497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(479..497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(511..527)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(511..527)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(524..546)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    535..551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(559..565,566..583,584..589))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    604..625
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(742..759)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(913..1501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(993..1012)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1672..2463,2464..2532))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2295..2314
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2533..2637)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2664..3119
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2751..2770)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2961..2982
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3245..3267
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3259..3276
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3260..3276
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        3437..3816
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3817..4020
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3971..3991
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4044..4053
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4053..4076
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    join(11692..12232,1..82)
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(11891..11910)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca
       61 ctcagtagat gcctgttgaa ttgggtacgc ggccagcttg gctgtggaat gtgtgtcagt
      121 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
      181 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
      241 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
      301 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
      361 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
      421 gaggcctagg cttttgcaaa aagctcctcg aggaactgaa aaaccagaaa gttaattccc
      481 tatagtgagt cgtattaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
      541 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      601 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      661 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      721 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      781 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      841 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      901 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      961 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1021 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1081 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1141 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1201 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1261 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1321 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1381 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1441 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1501 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1561 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1621 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1681 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1741 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1801 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1861 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1921 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1981 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2041 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2101 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2161 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2221 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2281 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2341 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2401 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2461 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2521 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2581 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2641 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     2701 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     2761 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     2821 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     2881 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     2941 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3001 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3061 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc
     3121 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     3181 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     3241 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctgatcta tacattgaat
     3301 caatattggc aattagccat attagtcatt ggttatatag cataaatcaa tattggctat
     3361 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca
     3421 atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     3481 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     3541 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     3601 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     3661 cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac
     3721 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg
     3781 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc
     3841 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     3901 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc
     3961 gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     4021 cgtttagtga accgtcagaa ttggccacca tggactacaa ggacgatgac gataagggat
     4081 ccatggctag tggcagctgt caggggtgcg aagaggacga ggaaactctg gcagcattga
     4141 tagtcaggct gaacaatgtc caggaaggaa aacagataga aacgctggtc caaatcctgg
     4201 aggatctgct ggtgttcacg tactccgagc acgcctccaa gttatttcaa ggcaaaaata
     4261 tccatgtgcc tctgttgatc gtcttggact cctatatgag agtcgcgagt gtgcagcagg
     4321 tgggttggtc acttctgtgc aaattaatag aagtctgtcc aggtacaatg caaagcttaa
     4381 tgggacccca ggatgttgga aatgattggg aagtccttgg tgttcaccaa ttgattctta
     4441 aaatgctaac agttcataat gccagtgtaa acttgtcagt gattggactg aagaccttag
     4501 atctcctcct aacttcaggt aaaatcacct tgctgatatt ggatgaagaa agtgatattt
     4561 tcatgttaat ttttgatgcc atgcactcat ttccagccaa tgatgaagtc cagaaacttg
     4621 gatgcaaagc tttacatgtg ctgtttgaga gagtctcaga ggagcaactg actgaatttg
     4681 ttgagaacaa agattatatg atattgttaa gtgcgttaac aaattttaaa gatgaagagg
     4741 aaattgtgct tcatgtgctg cattgtttac attccctagc gattccttgc aataatgtgg
     4801 aagtcctcat gagtggcaat gtcaggtgtt ataatattgt ggtggaagct atgaaagcat
     4861 tccctatgag tgaaagaatt caagaagtga gttgctgttt gctccatagg cttacattag
     4921 gtaatttttt caatatcctg gtattaaacg aagtccatga gtttgtggtg aaagctgtgc
     4981 agcagtaccc agagaatgca gcattgcaga tctcagcgct cagctgtttg gccctcctca
     5041 ctgagactat tttcttaaat caagatttag aggaaaagaa tgagaatcaa gagaatgatg
     5101 atgaggggga agaagataaa ttgttttggc tggaagcctg ttacaaagca ttaacgtggc
     5161 atagaaagaa caagcacgtg caggaggccg catgctgggc actaaataat ctccttatgt
     5221 accaaaacag tttacatgag aagattggag atgaagatgg ccatttccca gctcataggg
     5281 aagtgatgct ctccatgctg atgcattctt catcaaagga agttttccag gcatctgcga
     5341 atgcattgtc aactctctta gaacaaaatg ttaatttcag aaaaatactg ttatcaaaag
     5401 gaatacacct gaatgttttg gagttaatgc agaagcatat acattctcct gaagtggctg
     5461 aaagtggctg taaaatgcta aatcatcttt ttgaaggaag caacacttcc ctggatataa
     5521 tggcagcagt ggtccccaaa atactaacag ttatgaaacg tcatgagaca tcattaccag
     5581 tgcagctgga ggcgcttcga gctattttac attttatagt gcctggcatg ccagaagaat
     5641 ccagggagga tacagaattt catcataagc taaatatggt taaaaaacag tgtttcaaga
     5701 atgatattca caaactggtc ctagcagctt tgaacaggtt cattggaaat cctgggattc
     5761 agaaatgtgg attaaaagta atttcttcta ttgtacattt tcctgatgca ttagagatgt
     5821 tatccctgga aggtgctatg gattcagtgc ttcacacact gcagatgtat ccagatgacc
     5881 aagaaattca gtgtctgggt ttaagtctta taggatactt gattacaaag aagaatgtgt
     5941 tcataggaac tggacatctg ctggcaaaaa ttctggtttc cagcttatac cgatttaagg
     6001 atgttgctga aatacagact aaaggatttc agacaatctt agcaatcctc aaattgtcag
     6061 catctttttc taagctgctg gtgcatcatt catttgactt agtaatattc catcaaatgt
     6121 cttccaatat catggaacaa aaggatcaac agtttctaaa cctctgttgc aagtgttttg
     6181 caaaagtagc tatggatgat tacttaaaaa atgtgatgct agagagagcg tgtgatcaga
     6241 ataacagcat catggttgaa tgcttgcttc tattgggagc agatgccaat caagcaaagg
     6301 agggatcttc tttaatttgt caggtatgtg agaaagagag cagtcccaaa ttggtggaac
     6361 tcttactgaa tagtggatct cgtgaacaag atgtacgaaa agcgttgacg ataagcattg
     6421 ggaaaggtga cagccagatc atcagcttgc tcttaaggag gctggccctg gatgtggcca
     6481 acaatagcat ttgccttgga ggattttgta taggaaaagt tgaaccttct tggcttggtc
     6541 ctttatttcc agataagact tctaatttaa ggaaacaaac aaatatagca tctacactag
     6601 caagaatggt gatcagatat cagatgaaaa gtgctgtgga agaaggaaca gcctcaggca
     6661 gcgatggaaa tttttctgaa gatgtgctgt ctaaatttga tgaatggacc tttattcctg
     6721 actcttctat ggacagtgtg tttgctcaaa gtgatgacct ggatagtgaa ggaagtgaag
     6781 gctcatttct tgtgaaaaag aaatctaatt caattagtgt aggagaattt taccgagatg
     6841 ccgtattaca gcgttgctca ccaaatttgc aaagacattc caattccttg gggcccattt
     6901 ttgatcatga agatttactg aagcgaaaaa gaaaaatact atcttcagat gattcactca
     6961 ggtcatcaaa acttcaatcc catatgaggc attcagacag catttcttct ctggcttctg
     7021 agagagaata tattacatca ctagaccttt cagcaaatga actaagagat attgatgccc
     7081 taagccagaa atgctgtata agtgttcatt tggagcatct tgaaaagctg gagcttcacc
     7141 agaatgcact cacgagcttt ccacaacagc tatgtgaaac tctgaagagt ttgacacatt
     7201 tggacttgca cagtaataaa tttacatcat ttccttctta tttgttgaaa atgagttgta
     7261 ttgctaatct tgatgtctct cgaaatgaca ttggaccctc agtggtttta gatcctacag
     7321 tgaaatgtcc aactctgaaa cagtttaacc tgtcatataa ccagctgtct tttgtacctg
     7381 agaacctcac tgatgtggta gagaaactgg agcagctcat tttagaagga aataaaatat
     7441 cagggatatg ctcccccttg agactgaagg aactgaagat tttaaacctt agtaagaacc
     7501 acatttcatc cctatcagag aactttcttg aggcttgtcc taaagtggag agtttcagtg
     7561 ccagaatgaa ttttcttgct gctatgcctt tcttgcctcc ttctatgaca atcctaaaat
     7621 tatctcagaa caaattttcc tgtattccag aagcaatttt aaatcttcca cacttgcggt
     7681 ctttagatat gagcagcaat gatattcagt acctaccagg tcccgcacac tggaaatctt
     7741 tgaacttaag ggaactctta tttagccata atcagatcag catcttggac ttgagtgaaa
     7801 aagcatattt atggtctaga gtagagaaac tgcatctttc tcacaataaa ctgaaagaga
     7861 ttcctcctga gattggctgt cttgaaaatc tgacatctct ggatgtcagt tacaacttgg
     7921 aactaagatc ctttcccaat gaaatgggga aattaagcaa aatatgggat cttcctttgg
     7981 atgaactgca tcttaacttt gattttaaac atataggatg taaagccaaa gacatcataa
     8041 ggtttcttca acagcgatta aaaaaggctg tgccttataa ccgaatgaaa cttatgattg
     8101 tgggaaatac tgggagtggt aaaaccacct tattgcagca attaatgaaa accaagaaat
     8161 cagatcttgg aatgcaaagt gccacagttg gcatagatgt gaaagactgg cctatccaaa
     8221 taagagacaa aagaaagaga gatctcgtcc taaatgtgtg ggattttgca ggtcgtgagg
     8281 aattctatag tactcatccc cattttatga cgcagcgagc attgtacctt gctgtctatg
     8341 acctcagcaa gggacaggct gaagttgatg ccatgaagcc ttggctcttc aatataaagg
     8401 ctcgcgcttc ttcttcccct gtgattctcg ttggcacaca tttggatgtt tctgatgaga
     8461 agcaacgcaa agcctgcatg agtaaaatca ccaaggaact cctgaataag cgagggttcc
     8521 ctgccatacg agrttaccac tttgtgaatg ccaccgagga atctgatgct ttggcaaaac
     8581 ttcggaaaac catcataaac gagagcctta atttcaagat ccgagatcag cttgttgttg
     8641 gacagctgat tccagactgc tatgtagaac ttgaaaaaat cattttatcg gagcgtaaaa
     8701 atgtgccaat tgaatttccc gtaattgacc ggaaacgatt attacaacta gtgagagaaa
     8761 atcagctgca gttagatgaa aatgagcttc ctcacgcagt tcactttcta aatgaatcag
     8821 gagtccttct tcattttcaa gacccagcac tgcagttaag tgacttgtac tttgtggaac
     8881 ccaagtggct ttgtaaaatc atggcacaga ttttgacagt gaaagtggaa ggttgtccaa
     8941 aacaccctaa gggaattatt tcgcgtagag atgtggaaaa atttctttca aagaaaagga
     9001 aatttccaaa gaactacatg acacagtatt ttaagctcct agaaaaattc cagattgctt
     9061 tgccaatagg agaagaatat ttgctggttc caagcagttt gtctgaccac aggcctgtga
     9121 tagagcttcc ccattgtgag aactctgaaa ttatcatccg actatatgaa atgccttatt
     9181 ttccaatggg attttggtca agattaatca atcgattact tgagatttca ccttacatgc
     9241 tttcagggag agaacgagca cttcgcccaa acagaatgta ttggcgacaa ggcatttact
     9301 taaattggtc tcctgaagct tattgtctgg taggatctga agtcttagac aatcatccag
     9361 agagtttctt aaaaattaca gttccttctt gtagaaaagg ctgtattctt ttgggccaag
     9421 ttgtggacca cattgattct ctcatggaag aatggtttcc tgggttgctg gagattgata
     9481 tttgtggtga aggagaaact ctgttgaaga aatgggcatt atatagtttt aatgatggtg
     9541 aagaacatca aaaaatctta cttgatgact tgatgaagaa agcagaggaa ggagatctct
     9601 tagtaaatcc agatcaacca aggctcacca ttccaatatc tcagattgcc cctgacttga
     9661 ttttggctga cctgcctaga aatattatgt tgaataatga tgagttggaa tttgaacaag
     9721 ctccagagtt tctcctaggt gatggcagtt ttggatcagt ttaccgagca gcctatgaag
     9781 gagaagaagt ggctgtgaag atttttaata aacatacatc actcaggctg ttaagacaag
     9841 agcttgtggt gctttgccac ctccaccacc ccagtttgat atctttgctg gcagctggga
     9901 ttcgtccccg gatgttggtg atggagttag cctccaaggg ttccttggat cgcctgcttc
     9961 agcaggacaa agccagcctc actagaaccc tacagcacag gattgcactc cacgtagctg
    10021 atggtttgag atacctccac tcagccatga ttatataccg agacctgaaa ccccacaatg
    10081 tgctgctttt cacactgtat cccaatgctg ccatcattgc aaagattgct gactacggca
    10141 ttgctcagta ctgctgtaga atggggataa aaacatcaga gggcacacca gggtttcgtg
    10201 cacctgaagt tgccagagga aatgtcattt ataaccaaca ggctgatgtt tattcatttg
    10261 gtttactact ctatgacatt ttgacaactg gaggtagaat agtagagggt ttgaagtttc
    10321 caaatgagtt tgatgaatta gaaatacaag gaaaattacc tgatccagtt aaagaatatg
    10381 gttgtgcccc atggcctatg gttgagaaat taattaaaca gtgtttgaaa gaaaatcctc
    10441 aagaaaggcc tacttctgcc caggtctttg acattttgaa ttcagctgaa ttagtctgtc
    10501 tgacgagacg cattttatta cctaaaaacg taattgttga atgcatggtt gctacacatc
    10561 acaacagcag gaatgcaagc atttggctgg gctgtgggca caccgacaga ggacagctct
    10621 catttcttga cttaaatact gaaggataca cttctgagga agttgctgat agtagaatat
    10681 tgtgcttagc cttggtgcat cttcctgttg aaaaggaaag ctggattgtg tctgggacac
    10741 agtctggtac tctcctggtc atcaataccg aagatgggaa aaagagacat accctagaaa
    10801 agatgactga ttctgtcact tgtttgtatt gcaattcctt ttccaagcaa agcaaacaaa
    10861 aaaattttct tttggttgga accgctgatg gcaagttagc aatttttgaa gataagactg
    10921 ttaagcttaa aggagctgct cctttgaaga tactaaatat aggaaatgtc agtactccat
    10981 tgatgtgttt gagtgaatcc acaaattcaa cggaaagaaa tgtaatgtgg ggaggatgtg
    11041 gcacaaagat tttctccttt tctaatgatt tcaccattca gaaactcatt gagacaagaa
    11101 caagccaact gttttcttat gcagctttca gtgattccaa catcataaca gtggtggtag
    11161 acactgctct ctatattgct aagcaaaata gccctgttgt ggaagtgtgg gataagaaaa
    11221 ctgaaaaact ctgtggacta atagactgcg tgcacttttt aagggaggta atggtaaaag
    11281 aaaacaagga atcaaaacac aaaatgtctt attctgggag agtgaaaacc ctctgccttc
    11341 agaagaacac tgctctttgg ataggaactg gaggaggcca tattttactc ctggatcttt
    11401 caactcgtcg acttatacgt gtaatttaca acttttgtaa ttcggtcaga gtcatgatga
    11461 cagcacagct aggaagcctt aaaaatgtca tgctggtatt gggctacaac cggaaaaata
    11521 ctgaaggtac acaaaagcag aaagagatac aatcttgctt gaccgtttgg gacatcaatc
    11581 ttccacatga agtgcaaaat ttagaaaaac acattgaagt gagaaaagaa ttagctgaaa
    11641 aaatgagacg aacatctgtt gagtaagaga gaaataggcg gccgcgatcc cgggtggcat
    11701 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
    11761 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
    11821 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
    11881 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
    11941 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
    12001 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
    12061 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
    12121 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
    12181 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tg
//
