LOCUS       sequence-37450-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Piatkevich KD, Hulit J, Subach OM, Wu B, Abdulla A, Segall JE, 
            Verkhusha VV
  TITLE     Monomeric red fluorescent proteins with a large Stokes shift.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5369-74. Epub 2010 Mar
            8.
  PUBMED    20212155
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2010-03-23"; volume: "107(12)"; pages: 
            "5369-74. Epub 2010 Mar 8"
COMMENT     Sequence Label: pLSSmKate2-C1
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..738
                     /codon_start=1
                     /product="monomeric photoactivatable red fluorescent 
                     protein (Gunewardene et al., 2011)"
                     /label=PAmKate
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSYTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAGTEMLYPADGGLEGRSD
                     DALKLVGGGHLICNLKSTYRSKKPAKNLKVPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    739..804
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 ccgtcagatc cgctagcggt ggccaccatg gtgtctaagg gcgaagagct gattaaggag
       61 aacatgcaca tgaagctgta catggaaggc accgtgaaca accaccactt caagtgcaca
      121 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
      181 ggcggccctc tacccttcgc cttcgacatc ttggctacca gcttcatgta cggcagctac
      241 accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt ccctgagggc
      301 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
      361 accagcctcc aggacggttg cctcatctac aacgtcaaga tcagaggggt gaacttcaca
      421 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccggcac cgagatgctg
      481 taccccgctg acggcggcct ggaaggcaga tcggacgacg ccctgaagct cgtgggcggg
      541 ggccacctga tctgcaactt gaagagcaca tacagatcca agaaacccgc taagaatctc
      601 aaggtgcccg gcgtctacta tgtggaccga agactggaaa gaatcaagga ggccgacaaa
      661 gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
      721 ctggggcaca agcttaattc cggactcaga tctcgagctc aagcttcgaa ttctgcagtc
      781 gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc
      841 aca
//
