LOCUS       sequence-37451-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Valastyan JS, Lindquist S
  TITLE     TorsinA and the TorsinA-Interacting Protein Printor Have No Impact 
            on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast.
  JOURNAL   PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26.
  PUBMED    21818381
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22744. Epub 2011 Jul 26"
COMMENT     Sequence Label: 413-Met25 Kar2 TorsinADE HDEL
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        72..421
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     422..438
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     422..438
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    446..470
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agggcattac cctcctaaag ggaacaaaag ctggagctcc ggatgcaagg gttcgaatcc
       61 cttagctctc attatttttt gctttttctc ttgaggtcac atgatcgcaa aatggcaaat
      121 ggcacgtgaa gctgtcgata ttggggaact gtggtggttg gcaaatgact aattaagtta
      181 gtcaaggcgc catcctcatg aaaactgtgt aacataataa ccgaagtgtc gaaaaggtgg
      241 caccttgtcc aattgaacac gctcgatgaa aaaaataaga tatatataag gttaagtaaa
      301 gcgtctgtta gaaaggaagt ttttcctttt tcttgctctc ttgtcttttc atctactatt
      361 tccttcgtgt aatacagggt cgtcagatac atagatacaa ttctattacc cccatccata
      421 ctctagaact agtggatccc ccatcacaag tttgtacaaa aaagcaggct tcacaaaatg
      481 tttttcaaca gactaagcgc tggcaagctg ctggtaccac tctccgtggt cctgtacgcc
      541 cttttcgtgg taatattacc tttacagaat tctttccact cctccaatgt tttagttaga
      601 ggtgtggagc ccatcagcct gggactggcc ctggccggcg tcctcaccgg ctacatctac
      661 ccgcgtctct actgcctctt cgccgagtgc tgcgggcaga agcggagcct tagccgggag
      721 gcactgcaga aggatctgga cgacaacctc tttggacagc atcttgcaaa gaaaatcatc
      781 ttaaatgccg tgtttggttt cataaacaac ccaaagccca agaaacctct cacgctctcc
      841 ctgcacgggt ggacaggcac cggcaaaaat ttc
//
