LOCUS       sequence-374513-       13199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of RNA A-to-I editor rABE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13199)
  AUTHORS   Lin Y, Kwok S, Hein AE, Thai BQ, Alabi Y, Ostrowski MS, Wu K, Floor 
            SN
  TITLE     RNA molecular recording with an engineered RNA deaminase.
  JOURNAL   Nat Methods. 2023 Dec;20(12):1887-1899. doi: 
            10.1038/s41592-023-02046-z. Epub 2023 Oct 19.
  PUBMED    37857907
REFERENCE   2  (bases 1 to 13199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-02046-z"; journalName: "Nat Methods"; date: 
            "2023-12"; volume: "20"; issue: "12"; pages: "1887-1899"
COMMENT     Sequence Label: pSLIK-rABE-Flag
FEATURES             Location/Qualifiers
     source          1..13199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1780..1804
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1836..2150
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1844..1862
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1880..1898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1915..1933
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1951..1969
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1987..2005
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2022..2040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2058..2076
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2123..2147
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2687..2710
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2728..2752)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2802..4013
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3850..3869
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4138..4755,4756..4761,4762..4797,4798..4800,4801..4836,
                     4837..4839,4840..4875,4876..4884)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4937..5509
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5103..5120)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5330..5349
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5568..6587
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5568..6587
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    7053..7641
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7106..7126)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7524..7535)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7644..7660)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7645..7661)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8170..8350
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8376..8393)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8382..8606
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8652..9080
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8739..8758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8949..8970
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9089..9109)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9094..9423
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9274..9409
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9336..9355
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9471..9518
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9537..9911
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10041..10162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10078..10097)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10132..10151
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10211..10227)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10211..10227)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10224..10246)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10235..10251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10259..10265,10266..10283,10284..10289))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10304..10325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10442..10459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10613..11201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10693..10712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11372..12163,12164..12232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11995..12014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12233..12337)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12412..12431)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12603..12982
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12984..13182
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccagtttggt taattctaga cagttatcaa caagtttgta caaaaaagca
     1801 ggctttaaag gaaccaattc agtcgactgg atcccgagtt tactccctat cagtgataga
     1861 gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
     1921 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     1981 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
     2041 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
     2101 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc actagtccac
     2161 catgtctgag gtggaattct cgcacgaata ctggatgaga cacgcattga ctctcgcaaa
     2221 gagggctcgg gatgaacgcg aggtgcccgt gggggcagta ctcgtgctta acaatcgcgt
     2281 aatcggcgaa ggttggaata gggcgatcgg actccacgac cccactgcac atgcggaaat
     2341 catggccctt cgacagggag ggcttgtgat gcagaattat cgacttatcg atgcgacgct
     2401 gtacgtcacg tttgaacctt gcgtaatgtg cgcgggagct atgattcact cccgcattgg
     2461 acgagttgta ttcggtgtcc gcaacgccaa gacgggtgcc gcaggttcac tgatggacgt
     2521 gctgcattac ccaggcatga accaccgggt agaaatcaca gaaggcatat tggcggacga
     2581 atgtgcggcg ctgttgagct acttttttcg catgccgagg caggtattta atgcgcaaaa
     2641 gaaggctcag tcttcaacag atagcggtgg aagcagtgga ggctcagatt acaaggatga
     2701 cgatgacaag tgactcgaga tatctagacc cagctttctt gtacaaagtg gttgataaca
     2761 tctcgagcca taaagatggt taattaaccc acccaagatc tggcctccgc gccgggtttt
     2821 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2881 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2941 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     3001 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     3061 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     3121 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     3181 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     3241 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     3301 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     3361 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     3421 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     3481 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     3541 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     3601 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     3661 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     3721 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3781 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3841 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3901 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3961 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     4021 ggtaccgagc tcggatctcc accccgtacc ggtcctgcag gatttaaatg gcgcgccgct
     4081 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga attcaccatg
     4141 tctagactgg acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt
     4201 atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg
     4261 tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac
     4321 aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac
     4381 aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc
     4441 ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt
     4501 cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca
     4561 ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct
     4621 accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga
     4681 gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta
     4741 aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca
     4801 gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt
     4861 gaccttgaca tgctccccgg gtaactaagt aaggatccgc ggccgcacta gaggaattcc
     4921 gcccctctcc cccccccccc tctccctccc cccccctaac gttactggcc gaagccgctt
     4981 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     5041 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     5101 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     5161 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     5221 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5281 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     5341 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     5401 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     5461 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag cttaccggta
     5521 cgcgtgatag cttgccacaa cccgtaccaa agatggatag atccggaaag cctgaactca
     5581 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     5641 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     5701 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     5761 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     5821 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     5881 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     5941 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     6001 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     6061 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     6121 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     6181 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     6241 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     6301 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     6361 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     6421 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     6481 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     6541 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt agatgccgac
     6601 cgaacaagag ctgatttcga gaacgcctca gccagcaact cgcgcgagcc tagcaaggca
     6661 aatgcgagag aacggcctta cgcttggtgg cacagttctc gtccacagtt cgctaagctc
     6721 gctcggctgg gtcgcgggag ggccggtcgc agtgattcag gcccttctgg attgtgttgg
     6781 tccccagggc acgattgtca tgcccacgca ctcgggtgat ctgactgatc ccgcagattg
     6841 gagatcgccg cccgtgcctg ccgattgggt gcagatctct agatctgtac aagtaaagcg
     6901 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     6961 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttta
     7021 gtccctccca attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     7081 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7141 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7201 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7261 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7321 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7381 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7441 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7501 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7561 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7621 ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat
     7681 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     7741 caagaggagg aggaggtggg ttttccagtc acacttcagg tacctttaag accaatgact
     7801 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7861 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     7921 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     7981 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     8041 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     8101 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     8161 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8221 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8281 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8341 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     8401 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     8461 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     8521 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     8581 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     8641 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8701 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8761 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8821 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8881 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8941 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     9001 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     9061 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     9121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     9181 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9241 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9301 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9361 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9421 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     9481 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     9541 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     9601 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     9661 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     9721 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     9781 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     9841 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     9901 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     9961 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
    10021 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
    10081 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10141 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
    10201 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    10261 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    10321 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    10381 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    10441 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    10501 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    10561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10801 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    10861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    10921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    11041 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    11101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    11161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    11221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    11281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    11341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    11401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    11461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    11521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    11581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    11641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    11701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    11761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    11821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    11881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    11941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    12001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    12061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    12121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    12181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    12241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    12301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    12361 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
    12421 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
    12481 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
    12541 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
    12601 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
    12661 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
    12721 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
    12781 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    12841 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
    12901 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
    12961 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
    13021 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
    13081 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
    13141 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtact
//
