LOCUS       sequence-374517-        7240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A plasmid containing a planarian codon-optimized Nluc (sNluc1), 
            driven by the planarian GAPDH promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Hall RN, Weill U, Drees L, Leal-Ortiz S, Li H, Khariton M, Chai C, 
            Xue Y, Rosental B, Quake SR, Sanchez Alvarado A, Melosh NA, Fire AZ,
            Rink JC, Wang B
  TITLE     Heterologous reporter expression in the planarian Schmidtea 
            mediterranea through somatic mRNA transfection.
  JOURNAL   Cell Rep Methods. 2022 Sep 20;2(10):100298. doi: 
            10.1016/j.crmeth.2022.100298. eCollection 2022 Oct 24.
  PUBMED    36313809
REFERENCE   2  (bases 1 to 7240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2022-09-20"; volume: "2(10):100298. doi"; pages: "
            10.1016/j.crmeth.2022.100298. eCollection 2022 Oct 24"
COMMENT     Sequence Label: pGAPDH::sNluc1
FEATURES             Location/Qualifiers
     source          1..7240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    37..57
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3166..3190
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3710..3734)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4853..4873)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4881..4898)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4881..4897)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4890..4912)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5225..5247
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5285..5303)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5371..5475
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5476..5544,5545..6336)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5694..5713)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6507..7095
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6996..7015
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(7222..7240,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgctc
       61 aacaaagaaa tgtctggctc caaacaaaaa agtctttaaa ttaacaagtg tggaaaaccc
      121 aacttcacaa ttataggttg cacaaaacgg caataagttt ttattgccac attggaaagc
      181 ttaggatatt cagaattcac atgaaaccaa aaatcaatta aattcttttc gtgaaaaata
      241 attttcaaat aagaatctaa agttaactcc acaaggcaac cagattcctt cgatgattga
      301 gaattttcaa tttttttgat attttctaag ctctgaaaaa gatttttaat ccatttgaat
      361 tctgtttctt taggaaagta tttgttaaat tgactaccaa gttacctcaa ctgttttttc
      421 aaaccttaac aatttccttt ggaattggtt ttagcagtaa gttgttcacg atgaatatta
      481 caatgtgtat ggtaccatcg atttaactcg tacagagaga cccttcattt cttcagtcat
      541 agcagcttcg tcgcattacg aagaccaaag catcgtgccc agtcaatttc atttttgacg
      601 ataaaactat ttaaaactct gaaaatttct tctcctgtag ttttaccagg tagggctaag
      661 caaaaaagaa aatcgtcatg gacgcttcca caatactctg acaaaggtag gaatatgatc
      721 ttcattaacc aaatccgtag tttcatcaat ctgaatggaa aaatatatac ttttctatat
      781 cttggacatt agctgctttt ttacgtaatc agccatgaaa gttattcttc ttttaatagt
      841 attgtcgggt atagatattt tttcaataga acttttagct ttttcaccta acataatttt
      901 gtcattatct cagtagcagg ttttatcagg ttctatgcta cagtgtgatc tttcatattt
      961 ttcactatta acaatgacac cctgtatgaa gcctctgtag cattcacgtt ttgttgacca
     1021 accatatatt gtattgtttt tttatttcga ctaagttcat tggctttatt ttgaaaaaaa
     1081 gattgaggtt tttttgtgtg ctctttgtgc ttggattcaa aataccgctt tattttggaa
     1141 attttaaaac attcatttga gaatatttcg tagcacaaaa cgaactgtgg tcttggactt
     1201 tacgtaaatt ttaaaaattc ggtattgtat tttttcgtaa tagttttttt ctttatattt
     1261 gccatttgat atatttcctt ctgttgtacc aagttttcat cttcacgatc acttattgta
     1321 aaaattgttt catctttaac ttgaagatat gaatctttag agcttgcaac ttgaagattt
     1381 aagactcgtt cttttgtctt gttaggattt tggtcatcgg tgactttacg tttcaaaaaa
     1441 acctttttca gctagtgttc tatggtatga tccttaataa atatttaatt gttaatacag
     1501 aatataatac agacaggcac agcgacagac tgacagaaag atagatagac acacagacaa
     1561 caaacagaca gagcaataga cggacacaat tctaaaaaaa tgtttattca tattattgaa
     1621 aacctagctt taagaaacaa agtgcactta ataaaaagta aataaacacg atcatggcgc
     1681 gcgctttgta tgcctgtaag ataaactcct ccgaggaaac aagtacttga tatttttggg
     1741 ttttaaagtg tttaaatctt tttatttttc tgtttaatca ttgcgtttta aaggtttatg
     1801 cttgaaaaaa atttttggta gccaaccaag acacttttac ggcccaccat aaaagcagtc
     1861 gtggcccata ctttgggaaa cactgataaa aaagtgataa aaataaaata tttttttatt
     1921 aaatgtttga attgcattta gagcttgatt ttatacttat tttctaaaat gaattcattt
     1981 tcgataaatt tagagccaat ttggttaatt taagtgattt tcttagtttt aattgttttc
     2041 tattttttta atgttgttgt ttatttttat atttttttgt atctttttca acttttctat
     2101 tagttttgtc tcgtttatcg tcattttgat gatatttggt caattttagt ccatttgagt
     2161 tttccactgc aagttttttt ggctatcgtg ccagatcaac aaacttttga cattcccagt
     2221 ttttgcacaa caatatcgtc catgtatcca caacgaccaa accaatgcca atgccttaag
     2281 caaatgattt ggttatgacc atcagaagga gactcccagc atttaatact aggcttggaa
     2341 agtacgaaag aactcgcatt ataaaattga tagtggctcg tctatcaaaa caactaaaga
     2401 agacacctga acaattaaaa acctaaacat ttagataatt gattcctctt taaactaatg
     2461 tgaattttat aaaattataa tcactgtttc gatataaatt gattcgattt tgtataaaat
     2521 aagtagtttg ttaattcatt aatgttgata aaaacaaggg atctttgaat agttagaaca
     2581 tgaaaccttt ctacggtagc ccacggaccc ataaaattgt tagagtgtta gcagtcttga
     2641 tataacgact caccttttaa ataataccaa cgtcacttgt gacgggtgaa tagtggaaac
     2701 gtcttgttgc acgtgcactt gatagggatt cttttgtata tttttatcgt ctatcaatat
     2761 ccactgtgat taaatttaaa ttataatatt gatacttatt ttgtaatatt tatgaaatat
     2821 aattaataat caatatcttt ttaaatacat cgaaatttgt ctttcttata aatataaata
     2881 aaaagattaa ttatattctc atatttaggg attttaattc taattataaa atttgaatat
     2941 ataattgtgt aatcattgat tttttaatat gtaatgttta tcataacagt ttttaacact
     3001 tagcaaattt aacaattttt aaaaagttaa tcatagaact tgttttacta aatctacatt
     3061 atatttatat attttattta aacatagata taaaagtcta ggtaatcacg tacaacttta
     3121 ttaatttttg tagttatttt cttctctaga ttattattca atatggcaag tttgtacaaa
     3181 aaagcaggct taatggtttt tacattagaa gattttgttg gagattggag acaaacagct
     3241 ggatataatt tagatcaagt tttagaacaa ggaggagttt catcattgtt ccaaaatttg
     3301 ggagtttcag ttacaccaat tcaaagaatt gttttgagtg gagaaaatgg attaaagatt
     3361 gatattcatg ttattattcc atatgaggga ttatcaggag atcaaatggg acaaattgaa
     3421 aagattttca aagttgttta tccagttgat gatcatcatt tcaaagttat tttacattat
     3481 ggaacattag ttattgatgg agttacacca aatatgattg attattttgg aagaccatat
     3541 gagggaattg ctgtttttga tggaaagaag attacagtta caggaacatt atggaatgga
     3601 aacaaaatta ttgacgaaag attaattaat ccagatggat cattattatt tagagttaca
     3661 attaatggag ttacaggatg gagattatgt gaaagaattt tagcttaata cccagctttc
     3721 ttgtacaaag tggcttaaac tcttcgaaag ctttagtttg ggatagttta gatattttgg
     3781 acctgacttt aatatgttat aaatttaatg tggtttagtt aaataaattt ctttgtctcc
     3841 tatgatttat ttctttaatt aattttaata caattacatc atttttgtta attacattat
     3901 aaaatatatt aataattata tctagtaaat aacgagattg attagactaa tcataaatgt
     3961 acgtgcaaat ttttgatatt gcgcattttc ctgtttttaa aattaatttt gtattacttg
     4021 cactgaaatc aataaacaat tacttttaaa tcagttcaat ttggtattaa tttatcaaag
     4081 atttcaaata gtagataatt tatatattgt tttaccaata ttagccctag aaaacaattc
     4141 aaccagtcta gtaaaccttc ataaagtgtg cgctatgtat caatgacgca ttgtgctgtt
     4201 tgttactttt gcatcaacca ataaaaattc aggttcatta atttgcactt taatcaatta
     4261 aattttattt taaaatctgg attttttgtt ttttaaataa caattcgatc aaccatcaaa
     4321 aattcaggct catgcattaa atagataaaa ttcataaaac tgattaatta attttattta
     4381 ccctgttttt aaaatggctt gtgtatcaat caataaaaat tcaggtttat catttaacta
     4441 aagaaatagc taacttgctt gtatttgttc actgaatgtg ttatcatgga gccgatatgg
     4501 tgcctgcttg actggaagac tgaaatgctt ctgatgctca aatgttgtta tttcatagat
     4561 atcatgaagt cattcacatc atgtaggctt tggttaatgt actttcagct gattaatgta
     4621 ttttaaattc aagatgtaat ttctcaatgt cagcaccatt tttacggggg atgagcttga
     4681 tatgactcca gtctagagta tcaaagcttg ggtttcatta tgtctggccg agtcgacatc
     4741 attcagttat taattatttt accgaaatat agaatctagc ttaaatatat aggcaatagt
     4801 atataataat tttgaatttt ttaatcccca taaaataaaa tgtcaaaaga agcaacttta
     4861 ttatacatag ttgataattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4921 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4981 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     5041 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     5101 ctcagtgcaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     5161 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5221 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     5281 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5341 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5401 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5461 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5521 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5581 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5641 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5701 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5761 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5821 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5881 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5941 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6001 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6061 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6121 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6181 ggtgagcgtg gctctcgcgg tatcattgca gcactggggt cagatggtaa gccctcccgt
     6241 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6301 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6361 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6421 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6481 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6541 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6601 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6661 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6721 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6781 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6841 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6901 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6961 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7021 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7081 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt cttttgctgg
     7141 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7201 gcctttgagt gagctgatac cagcggataa caatttcaca
//
