LOCUS       sequence-374519-        3619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  M13 packaging signal cloned into the pSB1K3 vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Pujar A, Pathania A, Hopper C, Pandi A, Calderon CR, Fugger M, Nowak
            T, Kushwaha M
  TITLE     Phage-mediated intercellular CRISPRi for biocomputation in bacterial
            consortia.
  JOURNAL   Nucleic Acids Res. 2024 Dec 27:gkae1256. doi: 10.1093/nar/gkae1256.
  PUBMED    39727169
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Dec 27:gkae1256. doi: 10.1093/nar/gkae1256."
COMMENT     Sequence Label: pSB1K3_M13ps
FEATURES             Location/Qualifiers
     source          1..3619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(213..234)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     421..440
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     729..748
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    846..866
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     protein_bind    complement(877..910)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(1380..1397)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1380..1396)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1495..1512)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1666..2254)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1746..1765)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2376..3185)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(3186..3277)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3344..3363
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    3570..3603
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    join(3614..3619,1..14)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 gcggccgctt ctagggaacc ccggttgata atcagaaaag ccccaaaaac aggaagattg
       61 tataagcaaa tatttaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt
      121 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
      181 aagaatagcc cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
      241 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
      301 gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
      361 accctaaagg gagcccccga tttagagctt gacggggaaa gcgaacgtgg cgagaaagga
      421 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
      481 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggccgggatc tcgacgctct
      541 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
      601 gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
      661 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
      721 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
      781 ccggccacga tgcgtccggc gtagaggatc gagatcgatc tcgatcccgc gaaattaata
      841 cgatgtacta gtagcggccg ctgcagaaaa tgaagtgaag ttcctatact ttctagagaa
      901 taggaacttc tatagtgagt cgaataaggg cgacacaaaa tttattctaa atgcataata
      961 aatactgata acatcttata gtttgtatta tattttgtat tatcgttgac atgtataatt
     1021 ttgatatcaa aaactgattt tccctttatt attttcgaga tttattttct taattctctt
     1081 taacaaacta gaaatattgt atatacaaaa aatcataaat aatagatgaa tagtttaatt
     1141 ataggtgttc atcaatcgaa aaagcaacgt atcttattta aagtgcgttg cttttttctc
     1201 atttataagg ttaaataatt ctcatatatc aagcaaagtg acaggcgccc ttaaatattc
     1261 tgacaaatgc tctttcccta aactcccccc ataaaaaaac ccgccgaagc gggtttttac
     1321 gttatttgcg gattaacgat tactcgttat cagaaccgcc cagggggccc gagcttaaga
     1381 ctggccgtcg ttttacaaca cagaaagagt ttgtagaaac gcaaaaaggc catccgtcag
     1441 gggccttctg cttagtttga tgcctggcag ttccctactc tcgccttccg cttcctcgct
     1501 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1561 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1621 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1681 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1741 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1801 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1861 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1921 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1981 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2041 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tgggctaact acggctacac
     2101 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2161 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2221 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2281 gtctgacgct cagtggaacg acgcgcgcgt aactcacgtt aagggatttt ggtcatgagc
     2341 ttgcgccgtc ccgtcaagtc agcgtaatgc tctgcttaga aaaactcatc gagcatcaaa
     2401 tgaaattgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     2461 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     2521 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     2581 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     2641 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     2701 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgaggcg aaatacgcga
     2761 tcgctgttaa aaggacaatt acaaacagga atcgagtgca accggcgcag gaacactgcc
     2821 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaacgctgtt
     2881 tttccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     2941 atggtcggaa gtggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     3001 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     3061 tacaagcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     3121 tataaatcag catccatgtt ggaatttaat cgcggcctcg acgtttcccg ttgaatatgg
     3181 ctcatattct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3241 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggtcagtgt tacaaccaat
     3301 taaccaattc tgaacattat cgcgagccca tttatacctg aatatggctc ataacacccc
     3361 ttgtttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
     3421 gaaacgccgt agcgccgatg gtagtgtggg gactccccat gcgagagtag ggaactgcca
     3481 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgcccg ggctaattag
     3541 ggggtgtcgc ccttattcga ctctatagtg aagttcctat tctctagaaa gtataggaac
     3601 ttctgaagtg ggggaattc
//
