LOCUS       sequence-37452-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Valastyan JS, Lindquist S
  TITLE     TorsinA and the TorsinA-Interacting Protein Printor Have No Impact 
            on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast.
  JOURNAL   PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26.
  PUBMED    21818381
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22744. Epub 2011 Jul 26"
COMMENT     Sequence Label: 413-Met25 Kar2 TorsinA GFP
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        65..414
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     415..431
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     415..431
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    439..463
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcattncctc ctaagggaca aagctggagc tccggatgca agggttcgaa tcccttagct
       61 ctcattattt tttgcttttt ctcttgaggt cacatgatcg caaaatggca aatggcacgt
      121 gaagctgtcg atattgggga actgtggtgg ttggcaaatg actaattaag ttagtcaagg
      181 cgccatcctc atgaaaactg tgtaacataa taaccgaagt gtcgaaaagg tggcaccttg
      241 tccaattgaa cacgctcgat gaaaaaaata agatatatat aaggttaagt aaagcgtctg
      301 ttagaaagga agtttttcct ttttcttgct ctcttgtctt ttcatctact atttccttcg
      361 tgtaatacag ggtcgtcaga tacatagata caattctatt acccccatcc atactctaga
      421 actagtggat cccccatcac aagtttgtac aaaaaagcag gcttcacaaa atgtttttca
      481 acagactaag cgctggcaag ctgctggtac cactctccgt ggtcctgtac gcccttttcg
      541 tggtaatatt acctttacag aattctttcc actcctccaa tgttttagtt agaggtgtgg
      601 agcccatcag cctgggactg gccctggccg gcgtcctcac cggctacatc tacccgcgtc
      661 tctactgcct cttcgccgag tgctgcgggc agaagcggag ccttagccgg gaggcactgc
      721 agaaggatct ggacgacaac ctctttggac agcatcttgc aaagaaaatc atcttaaatg
      781 ccgtgtttgg tttcataaac aacccaaagc ccaagaaacc tctcacgctc tccctgcacg
      841 ggtggacagg caccggc
//
