LOCUS       sequence-374520-        2012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty pSEVA26x vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Pathania A, Hopper C, Pandi A, Fugger M, Nowak T, Kushwaha M
  TITLE     A synthetic communication system uncovers extracellular immunity 
            that self-limits bacteriophage transmission
  JOURNAL   bioRxiv (2022)
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2022)"
COMMENT     Sequence Label: pSEVA26x
FEATURES             Location/Qualifiers
     source          1..2012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(95..114)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             106..921
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            1088..1196
                     /note="incP origin of transfer"
     rep_origin      1243..1788
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1689..1708
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaatttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
       61 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcag
      121 cgtgaaacga gctgtagccg tccgcgtctg aacagcaaca tggatgcgga tctgtatggc
      181 tataaatggg cgcgtgataa cgtgggtcag agcggcgcga ccatttatcg tctgtatggc
      241 aaaccggatg cgccggaact gtttctgaaa catggcaaag gcagcgtggc gaacgatgtg
      301 accgatgaaa tggtgcgtct gaactggctg accgaattta tgccgctgcc gaccattaaa
      361 cattttattc gcaccccgga tgatgcgtgg ctgctgacca ccgcgattcc gggcaaaacc
      421 gcgtttcagg tgctggaaga atatccggat agcggcgaaa acattgtgga tgcgctggcc
      481 gtgtttctgc gtcgtctgca tagcattccg gtgtgcaact gcccgtttaa cagcgatcgt
      541 gtgtttcgtc tggcccaggc gcagagccgt atgaacaacg gcctggtgga tgcgagcgat
      601 tttgatgatg aacgtaacgg ctggccggtg gaacaggtgt ggaaagaaat gcataaactg
      661 ctgccgttta gcccggatag cgtggtgacc cacggcgatt ttagcctgga taacctgatt
      721 ttcgatgaag gcaaactgat tggctgcatt gatgtgggcc gtgtgggcat tgcggatcgt
      781 tatcaggatc tggccattct gtggaactgc ctgggcgaat ttagcccgag cctgcaaaaa
      841 cgtctgtttc agaaatatgg cattgataat ccggatatga acaaactgca atttcatctg
      901 atgctggatg aatttttcta ataattaatt ggaccgcggt ccgcgcgttg tccttttccg
      961 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     1021 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     1081 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     1141 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg
     1201 ccggccctag aaatatttta tctgattaat aagatgatct tcttgagatc gttttggtct
     1261 gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt tttcgaaggt
     1321 tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc agtcaccaaa
     1381 acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact cctctaaatc
     1441 aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg gactcaagac
     1501 gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca
     1561 gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga caaacgcggc
     1621 cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag
     1681 ccgccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt
     1741 gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc
     1801 gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa tctccgcccc
     1861 gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg
     1921 aggaagcgga atatatccgg cgcgagattg atttaccccg gttgataatc agaaaagccc
     1981 caaaaacagg aagattgtat aagcaaatat tt
//
