LOCUS       sequence-374525-        8952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an shRNA and mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Kokotos AC, Antoniazzi AM, Unda SR, Ko MS, Park D, Eliezer D, 
            Kaplitt MG, De Camilli P, Ryan TA
  TITLE     Phosphoglycerate kinase is a central leverage point in Parkinson's 
            disease-driven neuronal metabolic deficits.
  JOURNAL   Sci Adv. 2024 Aug 23;10(34):eadn6016. doi: 10.1126/sciadv.adn6016. 
            Epub 2024 Aug 21.
  PUBMED    39167658
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-08-23"; volume: "10(34):eadn6016. doi"; pages: " 
            10.1126/sciadv.adn6016. Epub 2024 Aug 21"
COMMENT     Sequence Label: pLKO.1 - TRC mTagBFP2
FEATURES             Location/Qualifiers
     source          1..8952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     77..94
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    211..232
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(247..252,253..270,271..277)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    285..301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     290..312
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     309..325
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     309..325
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     344..364
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        346..364
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        392..618
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             619..799
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    846..971
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1464..1697
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1882..1926
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2224..2472
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2224..2244
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2395..2414
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    4386..4503
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4552..5062
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4981..4999
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      5071..5080
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5077..5787
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     LTR             5862..6095
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6167..6288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6204..6223)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6258..6277
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6328..6463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6483..6502)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6484..6502)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6512..6529)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6512..6528)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6521..6543)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6670..7125
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6757..6776)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6967..6988
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7151..7255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7256..7324,7325..8116)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7474..7493)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8287..8875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8776..8795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
       61 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
      121 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
      181 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      241 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      301 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
      361 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
      421 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
      481 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
      541 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
      601 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      661 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      721 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
      781 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
      841 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
      901 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
      961 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     1021 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     1081 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     1141 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     1201 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     1261 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     1321 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     1381 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     1441 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     1501 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     1561 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     1621 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     1681 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     1741 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     1801 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     1861 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     1921 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     1981 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     2041 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     2101 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     2161 ttagtgaacg gatctcgacg gtatcgatca cgagactagc ctcgagcggc cgcccccttc
     2221 accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     2281 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     2341 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     2401 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     2461 ggacgaaaca ccggtgtgtt gtaaatgagc acacaaaata cacatgctaa aatattatat
     2521 tctatgacct ttataaaatc aaccaaaatc ttctttttaa taactttagt atcaataatt
     2581 agaattttta tgttcctttt tgcaaacttt taataaaaat gagcaaaata aaaaaacgct
     2641 agttttagta actcgcgttg ttttcttcac ctttaataat agctactcca ccacttgttc
     2701 ctaagcggtc agctcctgct tcaatcattt tttgagcatc ttcaaatgtt ctaactccac
     2761 cagctgcttt aactaaagca ttgtctttaa caactgactt cattagttta acatcttcaa
     2821 atgttgcacc tgattttgaa aatcctgttg atgttttaac aaattctaat ccagcttcaa
     2881 cagctatttc acaagctttc atgatttctt cttttgttaa taaacaattt tccataatac
     2941 atttaacaac atgtgatcca gctgcttttt ttacagcttt catgtcttct aaaactaatt
     3001 cataattttt gtcttttaat gcaccaatat ttaataccat atcaatttct gttgcaccat
     3061 ctttaattgc ttcagaaact tcgaatgctt ttgtagctgt tgtgcatgca cctagaggaa
     3121 aacctacaac atttgttatt cctacatttg tgccttttaa taattcttta caatagcttg
     3181 ttcaatatga attaacacaa actgttgcaa aatcaaattc aattgcttca tcacataatt
     3241 gtttaatttc agctttcgta gcatcttgtt ttaataatgt gtgatctata tatttgttta
     3301 gtttcatttt ttctcctata tattcatttt taattttaat tctttaataa tttcgtctac
     3361 tttaacttta gcgttttgaa cagattcacc aacacctata aaataaattt ttagtttagg
     3421 ttcagttcca cttgggcgaa cagcaaatca tgacttatct tctaaataaa attttagtaa
     3481 gtcttgtcct ggcatattat acattccatc gatgtagtct tcaacattaa caactttaag
     3541 tccagcaatt tgagttaagg gtgttgctct caatgatttc attaatggtt caatttttaa
     3601 tttcttttct tctggtttaa aattcaagtt taaagtgaaa gtgtaatatg cacccatttc
     3661 tttaaataaa tcttctaaat agtctactaa tgttttattt tgttttttat aaaatcaagc
     3721 agcctctgct attaatatag aagcttgtat tccatcttta tctctagctg agtcatcaat
     3781 tacatatcca taactttctt cataagcaaa aacaaaattt aatccgttat cttcttcttt
     3841 agcaatttct ctacccattc atttaaatcc agttaaagtt tttacaatat taactccata
     3901 tttttcatga gcgattctat cacccaaatc acttgttaca aaacttgaat atagagccgg
     3961 attttttgga atgctattta agcgttttag atttgataat tttcaatcaa ttaaaattgg
     4021 tcctgtttga tttccatcta atcttacaaa atgaccatca tgttttattg ccattccaaa
     4081 tctgtcagca tctgggtcat tcataataat aatatctgca tcatgtttaa taccatattc
     4141 aagcggtatt tttcatgcag gatcaaattc tggatttgga tttacaacat ttttaaatgt
     4201 ttcatcttca aatgcatgct cttcaacctc aataacgtta tatcctgatt cacgtaatat
     4261 ttttggggta aatttagttc ctgttccatt aactgcgcta aaaataattt ttaaatcttt
     4321 tttagcttct tgctcttttt tgtagaattc tcgacctcga gacaaatggc agtattcatc
     4381 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     4441 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     4501 tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga gggggttggg
     4561 gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
     4621 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
     4681 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
     4741 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
     4801 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
     4861 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
     4921 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
     4981 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
     5041 aatcaccgac ctctctcccc agggggatcc gccaccatgg tgtctaaggg cgaagagctg
     5101 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
     5161 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     5221 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
     5281 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
     5341 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
     5401 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     5461 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc
     5521 gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
     5581 gtgggcggga gccatctgat cgcaaacgcc aagaccacat atagatccaa gaaacccgct
     5641 aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag
     5701 gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc
     5761 cctagcaaac tggggcacaa gcttaattaa ggtaccttta agaccaatga cttacaaggc
     5821 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5881 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5941 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6001 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6061 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     6121 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     6181 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6241 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     6301 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6361 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6421 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     6481 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     6541 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     6601 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6661 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6721 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6781 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6841 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6901 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6961 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     7021 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7081 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     7141 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7201 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7261 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7321 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7381 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7441 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7501 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7561 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7621 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7681 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7741 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7801 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7861 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7921 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7981 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8041 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8101 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8161 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8401 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8881 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8941 tcctgcgtta tc
//
