LOCUS       sequence-374547-        1722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use 
            as the second 2A peptide in quad-cistronic construct assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Mukherjee M, Wang ZQ
  TITLE     A Well-Characterized Polycistronic-Like Gene Expression System in 
            Yeast.
  JOURNAL   Biotechnol Bioeng. 2022 Sep 27. doi: 10.1002/bit.28247.
  PUBMED    36168285
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2022 Sep 27. doi: 10.1002/bit.28247."
COMMENT     Sequence Label: pYTK001_O2Aq(2nd)
FEATURES             Location/Qualifiers
     source          1..1722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     336..355
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(623..657)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(728..1387)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1302..1321
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1569..1722,1..435)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
       61 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      121 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      181 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      241 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      301 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      361 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      421 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      481 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg
      541 ggtctcggtt ctggtaagtc caattacgac ctattgaagt tgtgtggaga tgttgaatcc
      601 aacccaggag gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
      661 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
      721 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
      781 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
      841 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
      901 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa
      961 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1021 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1081 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     1141 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     1201 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1261 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1321 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     1381 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     1441 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc
     1501 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1561 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1621 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1681 actggcttca gcagagcgca gataccaaat actgttcttc ta
//
