LOCUS       sequence-37455-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Valastyan JS, Lindquist S
  TITLE     TorsinA and the TorsinA-Interacting Protein Printor Have No Impact 
            on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast.
  JOURNAL   PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26.
  PUBMED    21818381
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22744. Epub 2011 Jul 26"
COMMENT     Sequence Label: 413-Met25 Kar2 TorsinADE K108A HDEL
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        67..416
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     417..433
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     417..433
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    441..465
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggcattacct cctaagggac aaaagctgga gctccggatg caagggttcg aatcccttag
       61 ctctcattat tttttgcttt ttctcttgag gtcacatgat cgcaaaatgg caaatggcac
      121 gtgaagctgt cgatattggg gaactgtggt ggttggcaaa tgactaatta agttagtcaa
      181 ggcgccatcc tcatgaaaac tgtgtaacat aataaccgaa gtgtcgaaaa ggtggcacct
      241 tgtccaattg aacacgctcg atgaaaaaaa taagatatat ataaggttaa gtaaagcgtc
      301 tgttagaaag gaagtttttc ctttttcttg ctctcttgtc ttttcatcta ctatttcctt
      361 cgtgtaatac agggtcgtca gatacataga tacaattcta ttacccccat ccatactcta
      421 gaactagtgg atcccccatc acaagtttgt acaaaaaagc aggcttcaca aaatgttttt
      481 caacagacta agcgctggca agctgctggt accactctcc gtggtcctgt acgccctttt
      541 cgtggtaata ttacctttac agaattcttt ccactcctcc aatgttttag ttagaggtgt
      601 ggagcccatc agcctgggac tggccctggc cggcgtcctc accggctaca tctacccgcg
      661 tctctactgc ctcttcgccg agtgctgcgg gcagaagcgg agccttagcc gggaggcact
      721 gcagaaggat ctggacgaca acctctttgg acagcatctt gcaaagaaaa tcatcttaaa
      781 tgccgtgttt ggtttcataa acaacccaaa gcccaagaaa cctctcacgc tctccctgca
      841 cgggtggaca ggcaccggcg ctaatttcgt c
//
