LOCUS       sequence-374563-       13550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing PE_CO-Mini (Prime editor with 
            codon-optimized and truncated Reverse transcriptase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13550)
  AUTHORS   Gao Z, Ravendran S, Mikkelsen NS, Haldrup J, Cai H, Ding X, Paludan 
            SR, Thomsen MK, Mikkelsen JG, Bak RO
  TITLE     A truncated reverse transcriptase enhances prime editing by split 
            AAV vectors.
  JOURNAL   Mol Ther. 2022 Jul 8. pii: S1525-0016(22)00422-1. doi: 
            10.1016/j.ymthe.2022.07.001.
  PUBMED    35808824
REFERENCE   2  (bases 1 to 13550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2022 Jul 8. pii: S1525-0016(22)00422-1. doi: 
            10.1016/j.ymthe.2022.07.001."
COMMENT     Sequence Label: LV-PE_CO-Mini
FEATURES             Location/Qualifiers
     source          1..13550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        82..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             680..860
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    907..1032
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1525..1758
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1943..1987
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2254..2371
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(2539..2787)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(2597..2616)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(2767..2787)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3057..3132
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3154..3653
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3189..3212)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3586..3605
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3707..3727
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3728..6241,6242..6244,6245..7828)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3728..7828
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9380..9400
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9401..9424
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9434..9490
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9491..9889
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(9958..9978)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     LTR             10180..10413
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10439..10456)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10445..10669
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10698..10717)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10699..10717)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10727..10744)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10727..10743)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10736..10758)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10885..11340
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10972..10991)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11182..11203
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11366..11470
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11471..11539,11540..12331)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11689..11708)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12502..13090
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12991..13010
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13244..13261
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13378..13399
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13414..13419,13420..13437,13438..13444)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13452..13468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13457..13479
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13476..13492
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13476..13492
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13511..13531
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13513..13531
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc
      721 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
      781 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
      841 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
      901 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
      961 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     1021 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     1081 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     1141 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     1201 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     1261 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     1321 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     1381 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1441 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1501 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1561 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1621 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1681 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1741 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     1801 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     1861 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     1921 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     1981 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     2041 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     2101 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     2161 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     2221 attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
     2281 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2341 acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc tcgacgacgc
     2401 aaaaaacaga ctgagcacgc tttgatggca gagcaccgac tcggtgccac tttttcaagt
     2461 tgataacgga ctagccttat ttcaacttgc tatgctgttt ccagcatagc tctgaaactc
     2521 acgtgctcag tctgggccgg tgtttcgtcc tttccacaag atatataaag ccaagaaatc
     2581 gaaatacttt caagttacgg taagcatatg atagtccatt ttaaaacata attttaaaac
     2641 tgcaaactac ccaagaaatt attactttct acgtcacgta ttttgtacta atatctttgt
     2701 gtttacagtc aaattaattc caattatctc tctaacagcc ttgtatcgta tatgcaaata
     2761 tgaaggaatc atgggaaata ggccctcgcg agcctgcagt atttagcatg ccccacccat
     2821 ctgcaaggca ttctggatag tgtcaaaaca gccggaaatc aagtccgttt atctcaaact
     2881 ttagcatttt gggaataaat gatatttgct atgctggtta aattagattt tagttaaatt
     2941 tcctgctgaa gctctagtac gataagtaac ttgacctaag tgtaaagttg agatttcctt
     3001 caggtttata tagcttgtgc gccgcctggg tacctcgtca accagtatcc cggtgcgttt
     3061 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
     3121 ccgagtcggt gcttttttgc gtggtcgagt accgggtagg ggaggcgctt ttcccaaggc
     3181 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     3241 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     3301 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     3361 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     3421 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     3481 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     3541 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     3601 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctcta
     3661 gcgagccacc atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg
     3721 gaaagtcgac aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc
     3781 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
     3841 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
     3901 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
     3961 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
     4021 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
     4081 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
     4141 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
     4201 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag
     4261 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa
     4321 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag
     4381 cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg
     4441 aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc
     4501 cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct
     4561 ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc
     4621 catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc
     4681 ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt
     4741 gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta
     4801 cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat
     4861 cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct
     4921 gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct
     4981 gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa
     5041 gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa
     5101 cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga
     5161 ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga
     5221 taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac
     5281 cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt
     5341 cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt
     5401 gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga
     5461 aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa
     5521 aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat
     5581 cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta
     5641 tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg
     5701 gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat
     5761 cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga
     5821 cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag
     5881 cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca
     5941 gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat
     6001 cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga
     6061 gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca
     6121 ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg
     6181 gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga
     6241 cgctatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag
     6301 aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat
     6361 gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa
     6421 tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag
     6481 acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat
     6541 gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa
     6601 gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa
     6661 caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa
     6721 aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg
     6781 gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta
     6841 cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa
     6901 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga
     6961 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga
     7021 ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct
     7081 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt
     7141 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag
     7201 tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat
     7261 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
     7321 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga
     7381 actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
     7441 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
     7501 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
     7561 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
     7621 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
     7681 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac
     7741 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
     7801 gatcgacctg tctcagctgg gaggtgactc tggaggatct agcggaggat cctctggcag
     7861 cgagacacca ggaacaagcg agtcagcaac accagagagc agtggcggca gcagcggcgg
     7921 cagcagcctg ggttctacat ggctgagcga cttccctcag gcctgggctg aaaccggcgg
     7981 catgggcctg gctgtgcggc aggctcctct gatcatccct ctgaaggcca cctccacccc
     8041 tgtgtccatc aagcagtacc ccatgagcca ggaggccaga ctgggcatca agccccacat
     8101 ccagcggctg ctcgaccagg gaatcctggt cccctgccag agcccctgga acaccccact
     8161 tttacctgtg aaaaagcccg gcactaacga ctaccggcct gtgcaggacc tgagagaagt
     8221 gaacaagcgg gttgaggaca tccaccctac agtgcctaac ccctacaacc tgctgtctgg
     8281 cctgccaccg agccaccagt ggtacaccgt gctggacctg aaagacgcct tcttctgtct
     8341 gagactccat cctaccagcc agccactgtt cgccttcgag tggcgggacc ctgagatggg
     8401 catcagcggc cagctgacat ggacccggct gccacagggc ttcaagaaca gccctacact
     8461 gttcaacgag gccctgcaca gagatctggc tgacttcaga atccagcatc ctgacctgat
     8521 cctgctgcag tacgtggacg acttgctgct tgccgctaca tctgaactgg attgccagca
     8581 aggcaccaga gctctgctcc agaccctggg caacctgggg tatagggcca gcgccaagaa
     8641 ggcccagatc tgccagaagc aagtgaagta cctgggatac ctgctgaagg aaggccagag
     8701 atggctgacc gaggcgcgga aggaaacagt gatgggccag cctaccccta agacccccag
     8761 acagctgcga gaattcctgg gcaaggctgg cttttgcaga ctgttcatcc ccggcttcgc
     8821 tgagatggcc gctcctctgt acccactgac caagcccggt accctgttca attggggccc
     8881 cgatcagcag aaagcctacc aggagattaa gcaggccctg ctgaccgccc ctgccctcgg
     8941 gctgcctgat ctgacaaaac ctttcgagct gtttgtcgac gagaagcagg gctacgccaa
     9001 gggcgtgctg acacagaaac tgggaccttg gagaagacct gtggcttatc tcagcaagaa
     9061 gctggacccc gtggccgccg gatggcctcc atgtctgagg atggtcgccg ctatcgccgt
     9121 gctgaccaag gacgccggca aactgaccat gggacaacct ctggtgatcc tggcccctca
     9181 cgccgtcgag gccctggtga agcagcctcc tgacagatgg ctgagcaacg ccagaatgac
     9241 ccactaccag gccctgctgc tggataccga tagagtgcag ttcggccctg tggtggctct
     9301 gaatcctgcc accctgctcc cacttcccga ggaaggctct ggcggctcaa aaagaaccgc
     9361 cgacggcagc gaattcgagc ccaagaagaa gaggaaagtc gattacaaag acgatgacga
     9421 taagggatcc ggcgcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa
     9481 tcctggaccg atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     9541 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     9601 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     9661 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     9721 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     9781 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     9841 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag aattccgatc
     9901 tgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     9961 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
    10021 ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga
    10081 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
    10141 ttccgtggtg tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
    10201 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
    10261 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    10321 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    10381 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
    10441 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
    10501 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
    10561 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
    10621 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggg cttttgcgtc
    10681 gagacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
    10741 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
    10801 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
    10861 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
    10921 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
    10981 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
    11041 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
    11101 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
    11161 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
    11221 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
    11281 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
    11341 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
    11401 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
    11461 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
    11521 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
    11581 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
    11641 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
    11701 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
    11761 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
    11821 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
    11881 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
    11941 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
    12001 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
    12061 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    12121 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    12181 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    12241 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    12301 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    12361 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    12421 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    12481 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    12541 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    12601 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    12661 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    12721 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    12781 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    12841 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    12901 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    12961 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    13021 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    13081 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    13141 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    13201 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    13261 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    13321 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    13381 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    13441 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    13501 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
//
