LOCUS       sequence-374580-       10326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid ensures constitutive expression of the C-terminal fragment 
            of split LYN kinase domain (aa 239-267), fused to the catalytically 
            inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS
            signal at the N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10326)
  AUTHORS   Rihtar E, Lebar T, Lainscek D, Kores K, Lesnik S, Bren U, Jerala R
  TITLE     Chemically inducible split protein regulators for mammalian cells.
  JOURNAL   Nat Chem Biol. 2022 Sep 26. doi: 10.1038/s41589-022-01136-x.
  PUBMED    36163385
REFERENCE   2  (bases 1 to 10326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 Sep 26. doi: 10.1038/s41589-022-01136-x."
COMMENT     Sequence Label: dCas9-NLS-gs10-cLYN268
FEATURES             Location/Qualifiers
     source          1..10326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(48..67)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        239..618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     773..793
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     867..886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        867..885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             957..974
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(975..998,999..1001,1002..3488,3489..3491,3492..5072)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGG"
     CDS             975..5072
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGG"
     CDS             5079..5099
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(5883..5901)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5884..5901)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5921..5938)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5927..6151
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6197..6625
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6284..6303)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6494..6515
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6634..6654)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6639..6968
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6819..6954
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6881..6900
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7035..7829
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRTAFYRLLDEFF"
     primer_bind     complement(7089..7108)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7699..7718
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8003..8124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8040..8059)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8094..8113
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8173..8189)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8173..8189)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8186..8208)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8197..8213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8221..8227,8228..8245,8246..8251))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8266..8287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8404..8421)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8575..9163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8655..8674)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9334..10125,10126..10194))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9957..9976
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10195..10299)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
       61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
      121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
      181 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
      241 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      301 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      361 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      421 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      481 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      541 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      601 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      661 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      721 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      781 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
      841 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg
      901 taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc accatgcacc
      961 accaccacca ccacgacaag aagtacagca tcggcctggc catcggcacc aactctgtgg
     1021 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
     1081 acaccgaccg gcacagcatc aagaagaacc tgatcggcgc cctgctgttc gacagcggag
     1141 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
     1201 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
     1261 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
     1321 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
     1381 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
     1441 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
     1501 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
     1561 aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga
     1621 gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc
     1681 tgttcggcaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
     1741 acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca
     1801 acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt
     1861 ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc
     1921 tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag
     1981 ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga
     2041 acggctacgc cggctacatc gatggcggag ccagccagga agagttctac aagttcatca
     2101 agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg
     2161 acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg
     2221 gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc
     2281 gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca
     2341 ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga
     2401 acttcgagga agtggtggac aagggcgcca gcgcccagag cttcatcgag cggatgacca
     2461 acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
     2521 acttcaccgt gtacaacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc
     2581 ccgccttcct gagcggcgag cagaaaaaag ccatcgtgga cctgctgttc aagaccaacc
     2641 ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact
     2701 ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc
     2761 tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg
     2821 aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga
     2881 aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca
     2941 ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca
     3001 agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga
     3061 tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg
     3121 gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca
     3181 tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg
     3241 agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca
     3301 gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga
     3361 aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc
     3421 agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg
     3481 atgtggacgc tatcgtgcct cagagctttc tgaaggacga ctccatcgat aacaaagtgc
     3541 tgactcggag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga
     3601 agaagatgaa gaactactgg cgccagctgc tgaatgccaa gctgattacc cagaggaagt
     3661 tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca
     3721 tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact
     3781 cccggatgaa cactaagtac gacgagaacg acaaactgat ccgggaagtg aaagtgatca
     3841 ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg
     3901 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc
     3961 tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg
     4021 acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact
     4081 tcttctacag caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga
     4141 tccggaagcg gcctctgatc gagacaaacg gcgaaacagg cgagatcgtg tgggataagg
     4201 gccgggactt tgccaccgtg cggaaagtgc tgtctatgcc ccaagtgaat atcgtgaaaa
     4261 agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg
     4321 acaagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc
     4381 ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac
     4441 tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga
     4501 atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca
     4561 agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg
     4621 ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt
     4681 acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc
     4741 tgtttgtgga acagcacaaa cactacctgg acgagatcat cgagcagatc agcgagttct
     4801 ccaagagagt gatcctggcc gacgctaatc tggacaaggt gctgagcgcc tacaacaagc
     4861 acagagacaa gcctatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
     4921 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca
     4981 ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
     5041 agacacggat cgacctgtct cagctgggag gctctgatcc aaaaaagaag agaaaggtag
     5101 gcggctctgg cggcggctcc ggaggctcta acagtaccaa ggtggctgtg aaaaccctga
     5161 agccaggaac tatgtctgtg caagccttcc tggaagaagc caacctcatg aagaccctgc
     5221 agcatgacaa gctcgtgagg ctctacgctg tggtcaccag ggaggagccc atttacatca
     5281 tcaccgagta catggccaag ggcagtttgc tggatttcct gaagagcgat gaaggtggca
     5341 aagtgctgct tccaaagctc attgactttt ctgctcagat tgcagaggga atggcataca
     5401 tcgagcggaa gaactacatt caccgggacc tgcgagcagc taatgttctg gtctccgagt
     5461 cactcatgtg caaaattgca gattttggcc ttgctagagt aattgaagat aatgagtaca
     5521 cagcaaggga aggtgctaag ttccctatta agtggacggc tccagaagca atcaactttg
     5581 gatgtttcac tattaagtct gatgtgtggt cctttggaat cctcctatac gaaattgtca
     5641 cctatgggaa aattccctac ccagggagaa ctaatgccga cgtgatgacc gccctgtccc
     5701 agggctacag gatgccccgt gtggagaact gcccagatga gctctatgac attatgaaaa
     5761 tgtgctggaa agaaaaggca gaagagagac caacgtttga ctacttacag agcgtcctgg
     5821 atgatttcta cacagccacg gaagggcaat accagcagca gccttaatct agagggccct
     5881 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
     5941 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     6001 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     6061 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     6121 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     6181 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6241 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6301 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6361 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6421 ggttcacgta gtgggccatc gccctgatag acggtttttc gccttttgac gttggagtcc
     6481 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6541 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6601 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     6661 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6721 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6781 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6841 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6901 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6961 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     7021 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     7081 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     7141 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     7201 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     7261 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     7321 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     7381 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     7441 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     7501 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     7561 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     7621 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     7681 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     7741 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcaccgcct
     7801 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     7861 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     7921 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     7981 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     8041 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8101 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     8161 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8221 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8281 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8341 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8401 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8461 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8521 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8581 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8641 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8701 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8761 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8821 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8881 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8941 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9001 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9061 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9121 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9181 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9241 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9301 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9361 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9421 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9481 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9541 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9601 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9661 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9721 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9781 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9841 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9901 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9961 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10021 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10081 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10141 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10201 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10261 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10321 acctga
//
