LOCUS       sequence-374585-        9835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock-in gene targeting in marmoset cells at FOXL2 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Yoshimatsu S, Kisu I, Qian E, Noce T
  TITLE     A New Horizon in Reproductive Research with Pluripotent Stem Cells: 
            Successful In Vitro Gametogenesis in Rodents, Its Application to 
            Large Animals, and Future In Vitro Reconstitution of Reproductive 
            Organs Such as ""Uteroid"" and ""Oviductoid"".
  JOURNAL   Biology (Basel). 2022 Jun 29;11(7):987. doi: 
            10.3390/biology11070987.
  PUBMED    36101367
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biology 
            (Basel)."; date: "2022-06-29"; volume: "11(7):987. doi"; pages: " 
            10.3390/biology11070987"
COMMENT     Sequence Label: pKI-cjFOXL2-EGFP-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..9835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(1819..2613)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1930..1949)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2540..2559
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(2957..3385)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3067..3088)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3279..3298
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3511..3533
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3525..3542
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3526..3542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3549..3568
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3549..3567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4176..4232
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4233..4235,4236..4238,4239..4952)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4233..4235,4236..4238,4239..4949)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4539..4558)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4886..4907
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4983..5016
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5112..5167
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     promoter        5538..6037
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5573..5596)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5970..5989
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6077..6676
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6077..6096)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6573..6593
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    7233..7266
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             8277..8849
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          8926..8991
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    9204..9259
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9615..9631)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9615..9631)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9628..9650)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9639..9655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9663..9669,9670..9687,9688..9693))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9708..9729
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaattc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     1861 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     1921 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     1981 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     2041 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     2101 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     2161 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     2221 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     2281 ctcggcagga gcaaggtggg atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     2341 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     2401 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
     2461 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     2521 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     2581 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     2641 atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     2701 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     2761 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     2821 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     2881 tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg
     2941 ccctgaattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3001 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3061 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3121 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     3181 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     3241 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     3301 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     3361 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     3421 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3481 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     3541 gtgaattgta atacgactca ctatagggcg aattgggccc tctagatgca tgctcgagcg
     3601 gctacctggc gccccccaag tacctgcaat ccggtttcct caacaactcg tggccgctgc
     3661 cgcagcctcc ctcacccatg ccctatgcct cctgccagat ggcggcggcc gcggcggctg
     3721 cggcggctgc ggctgcggcc gcgggcccgg gtagccctgg cgcggccgct gtggtcaagg
     3781 ggctggcggg cccggccgcc tcgtacgggc cgtacacacg cgtgcagagc atggcgctgc
     3841 cccccggcgt agtgaactcg tacaacggct tgggaggccc gccggccgcg cctccgcccc
     3901 cgccgcaccc ccacccgcat ccgcacgcac accatctgca cgcggccgcc gcaccgccgc
     3961 cggccccacc gcaccacggg gctgccgcac cgcccccggg ccagctcagc cccgccagcc
     4021 cagccaccgc cgcgcccccg gcgccggcgc ccagcagtgc gcccggcctg cagttcgctt
     4081 gcgcccggca gcccgagctc gccatgatgc actgctctta ctgggaccac gacagcaaga
     4141 cgggcgccct gcattcgcgc ctagatctcg ctagcgccac taacttctcc ctgttgaaac
     4201 aagcagggga tgtcgaagag aatcccgggc caatggtgag caagggcgag gagctgttca
     4261 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     4321 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     4381 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     4441 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4501 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4561 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4621 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4681 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     4741 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4801 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     4861 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4921 tcactctcgg catggacgag ctgtacaagt aagaattccg atcatattca ataaccctta
     4981 atataacttc gtataatgta tgctatacga agttattagg tctgaagagg agtttacgtc
     5041 cagccatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     5101 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     5161 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     5221 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     5281 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     5341 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     5401 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     5461 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagaagc ttaggatctc
     5521 gacctcgaaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     5581 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     5641 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     5701 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     5761 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     5821 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     5881 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     5941 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     6001 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatccatc tagatctcga
     6061 gcagctgaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     6121 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     6181 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     6241 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     6301 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     6361 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     6421 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     6481 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6541 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6601 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6661 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     6721 accccatgca tcgatgatat cagatccccg ggatgcagaa attgatgatc tattaaacaa
     6781 taaagatgtc cactaaaatg gaagtttttc ctgtcatact ttgttaagaa gggtgagaac
     6841 agagtaccta cattttgaat ggaaggattg gagctacggg ggtgggggtg gggtgggatt
     6901 agataaatgc ctgctcttta ctgaaggctc tttactattg ctttatgata atgtttcata
     6961 gttggatatc ataatttaaa caagcaaaac caaattaagg gccagctcat tcctcccact
     7021 catgatctat agatctatag atctctcgtg ggatcattgt ttttctcttg attcccactt
     7081 tgtggttcta agtactgtgg tttccaaatg tgtcagtttc atagcctgaa gaacgagatc
     7141 agcagcctct gttccacata cacttcattc tcagtattgt tttgccaagt tctaattcca
     7201 tcagaagctg gtcgagatcc ggaaccctta atataacttc gtataatgta tgctatacga
     7261 agttattagg tccctcgaag aggttcacta ggcgcggagc ccaccgcatg acagttcgga
     7321 cggatgcgag gatgcaggaa cgcgcggcgc cggccctggt cgcagccgca gccgccagcc
     7381 gaacctcgca ccctctgggg ccgcctctgg agccagtgcc cagggtccct ctgtgctttt
     7441 tcgctctcct cagctcctct cgcccctctt tgtccctctg cttgtcctgt gctcacctcc
     7501 gtgaccttta tgcccttgca ctcccccggc ctgaagccgc ctctctgcgc gctttctgct
     7561 gggctcttct ctttccggag ccccagcgtc tcctgcccaa attgaccgcg gaaagcgccc
     7621 aggaccgagg tgcgacccgg cgtcggcggc gcagacctct tggccttctc tcacaggtcg
     7681 gtgcgctagc gctcggcgtt ccccgcccga ctgccgtgca gtctttggct acacgcgccg
     7741 gactggattg gcggcgggac ccgcgccgcg ggagaaaggg acggaccaat ttgtgtgttt
     7801 gctccgcgaa ccctcttgaa ggtgttcaga agctgcttgc cgcggggccc actaggcgtt
     7861 gcgggggtta ggaaccagcg ggagccaggg cagcccggtc cgagctgtat tgtttcctgt
     7921 tcccaggggt cccaacactg ggctgcagat acgcaatcac tgctgcctcc ccaggagctc
     7981 ctggtcgagc agtgtggttt tcaagaggaa gcaaaaagcc tctccaccca ggcctggaat
     8041 gtttccaccc aatgtcgagc agtgtggttt tgcaagagga agcaaaaagc ctctccaccc
     8101 aggcctggaa tgtttccacc caatgtcgag caaaccccgc ccagcgtctt gtcattggcg
     8161 aattcgaaca cgcagatgca gtcggggcgg cgcggtccca ggtccacttc gcatattaag
     8221 gtgacgcgtg tggcctcgaa caccgagcga ccctgcaggt cctcgccatg gatcctgatg
     8281 atgttgttga ttcttctaaa tcttttgtga tggaaaactt ttcttcgtac cacgggacta
     8341 aacctggtta tgtagattcc attcaaaaag gtatacaaaa gccaaaatct ggtacacaag
     8401 gaaattatga cgatgattgg aaagggtttt atagtaccga caataaatac gacgctgcgg
     8461 gatactctgt agataatgaa aacccgctct ctggaaaagc tggaggcgtg gtcaaagtga
     8521 cgtatccagg actgacgaag gttctcgcac taaaagtgga taatgccgaa actattaaga
     8581 aagagttagg tttaagtctc actgaaccgt tgatggagca agtcggaacg gaagagttta
     8641 tcaaaaggtt cggtgatggt gcttcgcgtg tagtgctcag ccttcccttc gctgagggga
     8701 gttctagcgt tgaatatatt aataactggg aacaggcgaa agcgttaagc gtagaacttg
     8761 agattaattt tgaaacccgt ggaaaacgtg gccaagatgc gatgtatgag tatatggctc
     8821 aagcctgtgc aggaaatcgt gtcaggcgat ctctttgtga aggaacctta cttctgtggt
     8881 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     8941 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     9001 tatggaactg atgaatggga gcagtggtgg aatgcagatc cactagagtc gaagcggctt
     9061 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     9121 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     9181 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     9241 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     9301 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     9361 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     9421 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     9481 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     9541 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatcagctc ggtaccaagc
     9601 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9661 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9721 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9781 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgct
//
