LOCUS       sequence-374586-       10085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock-in gene targeting in marmoset cells at SF1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Yoshimatsu S, Kisu I, Qian E, Noce T
  TITLE     A New Horizon in Reproductive Research with Pluripotent Stem Cells: 
            Successful In Vitro Gametogenesis in Rodents, Its Application to 
            Large Animals, and Future In Vitro Reconstitution of Reproductive 
            Organs Such as ""Uteroid"" and ""Oviductoid"".
  JOURNAL   Biology (Basel). 2022 Jun 29;11(7):987. doi: 
            10.3390/biology11070987.
  PUBMED    36101367
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biology 
            (Basel)."; date: "2022-06-29"; volume: "11(7):987. doi"; pages: " 
            10.3390/biology11070987"
COMMENT     Sequence Label: pKI-cjSF1-hCD271-PGK-Neo
FEATURES             Location/Qualifiers
     source          1..10085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    80..101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(116..121,122..139,140..146)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    154..170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     159..181
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     178..194
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     178..194
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             806..862
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    2279..2400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2316..2335)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2370..2389
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2440..2473
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2517..3016
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2552..2575)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2949..2968
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3030..3833
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    4161..4194
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5164..5736
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          5813..5878
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(6026..6045)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6091..6146
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6145..6164)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6491..6510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6492..6510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6517..6534)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6517..6533)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6526..6548)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6674..7102
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6761..6780)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6971..6992
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             7446..8240
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(8258..8326,8327..9118)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8476..8495)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9289..9877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9778..9797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10031..10048
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
       61 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      121 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
      181 gaaacagcta tgaccatgat tacgccaagc ttcatgattc tcgttcattg tgaccacggt
      241 ggtgatgatg gcagtgatga tggtgacagg tgactgtgga tgggttgttg atggctggca
      301 ctggccacat ttatgaagag cacaggcacg tgggtgtcag tgacgatggt ggtgtttgtg
      361 aaccagtatg acaacaatgg tcatggtgac acggtcccag cagggagacc ctggttgcat
      421 ggttgatggt ggcagtggca agtcttcggt ggtcataggg aaggtggcat tgtgatgccg
      481 gtgacagtgt tggctgccca gccccaaagg ggatggatgg tgacccgatg gtgactgagc
      541 gcctcccttt gcagacgtga agttcctgaa taaccacagc ctggttaaag acgctcagga
      601 gaaggcgaac gctgccctgc tggactacac cctatgccac tacccacact gcggggacaa
      661 gtttcagcag ctgctgctgt gcctagtgga ggtgcgggcc ctgagcatgc aggccaagga
      721 gtacctgtac cacaagcacc tgggcaacga gatgccccgc aacaacctgc tcatcgagat
      781 gctgcaagct aagcagactg ctagcgccac taacttctcc ctgttgaaac aagcagggga
      841 tgtcgaagag aatcccgggc caatgggggc gggtgctact ggacgggcga tggatggccc
      901 gagacttctt cttttgcttc tcctcggggt tagtctggga ggcgcgaaag aagcgtgccc
      961 caccgggctg tacacacatt ccggcgaatg ctgtaaggcg tgtaatttgg gtgagggcgt
     1021 tgcacaacct tgcggcgcga accagactgt atgcgaacca tgcttggaca gtgtcacatt
     1081 ttcagacgtt gtgtcagcaa ccgaaccttg caaaccttgt acggagtgcg tcggtctgca
     1141 gtcaatgtcc gcgccctgcg ttgaagcgga tgatgctgtt tgcagatgcg cctacggata
     1201 ctatcaggac gagaccacgg gtcgctgtga ggcctgtagg gtatgcgaag cgggtagcgg
     1261 acttgtcttt tcatgtcaag ataagcaaaa caccgtgtgc gaagaatgcc cagacggtac
     1321 ctacagtgac gaagcaaatc acgttgatcc ctgcctcccc tgtactgtgt gcgaggatac
     1381 agagaggcag ttgagggaat gcacaaggtg ggctgatgcc gaatgcgagg agattcccgg
     1441 tcggtggatt actcgatcaa cccctcctga aggcagcgac tccaccgccc cgtcaaccca
     1501 agagccagag gccccgcctg agcaggattt gatagcgtcc acggtagcgg gtgttgttac
     1561 tacggtgatg gggtcaagcc aaccggtagt gacccgcggc acaactgata atctcattcc
     1621 tgtctattgt tcaatactgg cggctgtcgt cgtgggactt gttgcgtaca ttgcgttcaa
     1681 acgctggaac agttgcaagc aaaataagca aggagctaat tccaggccgg tcaaccaaac
     1741 tcctcctcct gaaggtgaaa aattgcattc cgatagtggt atatcagtag attcacagtc
     1801 tctccacgat cagcaaccgc atactcagac cgcgagtggt caagcattga agggtgacgg
     1861 gggactctac agctccctcc ctcccgcaaa gagagaagag gtcgagaagc tcctgaatgg
     1921 ctccgccgga gatacttggc gacacctcgc cggggaattg ggttaccaac cggaacatat
     1981 tgactctttt acgcatgaag cgtgcccagt cagagccctc cttgctagct gggcaactca
     2041 agactccgcg actcttgacg ccctcctcgc tgctttgaga cgaattcaaa gggctgattt
     2101 ggtagaatct ctttgttctg aatcaacagc cacgagcccc gtctgatgta caagtaggcg
     2161 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     2221 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     2281 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     2341 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     2401 aggcgttagg aattccgatc atattcaata acccttaata taacttcgta taatgtatgc
     2461 tatacgaagt tattaggtct gaagaggagt ttacgtccag ccaagctaat tctaccgggt
     2521 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     2581 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     2641 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     2701 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     2761 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     2821 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     2881 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     2941 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     3001 atctccgggc ctttcgacct gcagccaata tgggatcggc cattgaacaa gatggattgc
     3061 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     3121 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     3181 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     3241 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     3301 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     3361 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     3421 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     3481 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     3541 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     3601 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     3661 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     3721 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     3781 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaggggatc
     3841 cgctgtaagt ctgcagaaat tgatgatcta ttaaacaata aagatgtcca ctaaaatgga
     3901 agtttttcct gtcatacttt gttaagaagg gtgagaacag agtacctaca ttttgaatgg
     3961 aaggattgga gctacggggg tgggggtggg gtgggattag ataaatgcct gctctttact
     4021 gaaggctctt tactattgct ttatgataat gtttcatagt tggatatcat aatttaaaca
     4081 agcaaaacca aattaagggc cagctcattc ctcccactca tgatctatag atccctcgat
     4141 cgagatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc
     4201 cctcgtgagc ctgggcaggg ggcggggccg gggctggggg cggggccagg gcggggctgc
     4261 caccacccca ccggctccac gtgtttcatt ttccgaggcc cacccaggag ccccggcccc
     4321 gtccccgtcc cagaggccgc tgcccctgag ttctgacacg gtgtgtttgg gaaggtgggt
     4381 gaggctgggc agggcctggc agaggtgggg tggccctggc cagccaccag cacttgcctg
     4441 ctagttggag tgcccaaagg aggtggctgg taaccacccc cgccagcccc tgccctgctc
     4501 ccagatcttc agaatcgctc ctggattctg aagcctgcag gtcgggggag gaggttcggg
     4561 attccctagt gggcctcgat gtcccttgga tcagaggtca tcccttcctc ctctcctgaa
     4621 aacagacagg gagaagttga gcaggtatca actgggggag ggagagggtc tccagtgttc
     4681 ccctatggag aacaggaggg aggtcctctg ttttgtaaac taaggataac tgagtttgct
     4741 aaactgggag gggctactgg gccctagagg acagcaggag aactcgttag gacaaagagt
     4801 ccccctccct agcccttctg ctccacctga ctctgcaaga gggagctttg atcatcatca
     4861 ggaaagaact ttgctgcagt gtggttttca agaggaagca aaaagcctct ccacccaggc
     4921 ctggaatgtt tccacccaat gtcgagcagt gtggttttgc aagaggaagc aaaaagcctc
     4981 tccacccagg cctggaatgt ttccacccaa tgtcgagcaa accccgccca gcgtcttgtc
     5041 attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtcccaggt ccacttcgca
     5101 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcaggtcct cgccatggat
     5161 cctgatgatg ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac
     5221 gggactaaac ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt
     5281 acacaaggaa attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac
     5341 gctgcgggat actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc
     5401 aaagtgacgt atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact
     5461 attaagaaag agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa
     5521 gagtttatca aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct
     5581 gaggggagtt ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta
     5641 gaacttgaga ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat
     5701 atggctcaag cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt
     5761 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     5821 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     5881 tccaacctat ggaactgatg aatgggagca gtggtggaat gcagatccac tagagtcgaa
     5941 gcggcttcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
     6001 ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca
     6061 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     6121 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     6181 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     6241 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     6301 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     6361 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     6421 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tctctagagg
     6481 gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
     6541 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     6601 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     6661 gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6721 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6781 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6841 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6901 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6961 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     7021 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     7081 taacaaaaat ttaacgcgaa ttttaacaaa attcagggcg caagggctgc taaaggaagc
     7141 ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact
     7201 gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc
     7261 ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag
     7321 ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc
     7381 cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt
     7441 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     7501 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     7561 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     7621 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     7681 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     7741 ggcaggatct cctgtcatcc caccttgctc ctgccgagaa agtatccatc atggctgatg
     7801 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     7861 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     7921 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     7981 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     8041 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     8101 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     8161 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     8221 ttcttgacga gttcttctga attgaaaaag gaagagtatg agtattcaac atttccgtgt
     8281 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     8341 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     8401 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     8461 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     8521 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8581 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8641 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8701 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8761 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8821 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8881 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8941 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     9001 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     9061 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     9121 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     9181 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     9241 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     9301 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     9361 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9421 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9481 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9541 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9601 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9661 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9721 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9781 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9841 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9901 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9961 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    10021 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
    10081 tctcc
//
