LOCUS       sequence-374590-       10900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock-in gene targeting in marmoset cells at OSR1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Yoshimatsu S, Kisu I, Qian E, Noce T
  TITLE     A New Horizon in Reproductive Research with Pluripotent Stem Cells: 
            Successful In Vitro Gametogenesis in Rodents, Its Application to 
            Large Animals, and Future In Vitro Reconstitution of Reproductive 
            Organs Such as ""Uteroid"" and ""Oviductoid"".
  JOURNAL   Biology (Basel). 2022 Jun 29;11(7):987. doi: 
            10.3390/biology11070987.
  PUBMED    36101367
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biology 
            (Basel)."; date: "2022-06-29"; volume: "11(7):987. doi"; pages: " 
            10.3390/biology11070987"
COMMENT     Sequence Label: pKI-cjOSR1-EGFP-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..10900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(1819..2613)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1930..1949)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2540..2559
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(2957..3385)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3067..3088)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3279..3298
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3511..3533
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3525..3542
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3526..3542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3549..3568
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3549..3567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4612..4668
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4669..4671,4672..4674,4675..5388)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4669..4671,4672..4674,4675..5385)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4975..4994)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5322..5343
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    5419..5452
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5548..5603
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     promoter        5974..6473
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6009..6032)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6406..6425
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6513..7112
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6513..6532)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7009..7029
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    7669..7702
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             9342..9914
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          9991..10056
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    10269..10324
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10680..10696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10680..10696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10693..10715)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10704..10720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10728..10734,10735..10752,10753..10758))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10773..10794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaattc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     1861 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     1921 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     1981 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     2041 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     2101 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     2161 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     2221 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     2281 ctcggcagga gcaaggtggg atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     2341 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     2401 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
     2461 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     2521 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     2581 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     2641 atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     2701 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     2761 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     2821 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     2881 tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg
     2941 ccctgaattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3001 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3061 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3121 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     3181 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     3241 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     3301 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     3361 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     3421 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3481 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     3541 gtgaattgta atacgactca ctatagggcg aattgggccc tctagatgca tgctcgagcg
     3601 gccgcgtcct acaacctact tatccatgag cggacccaca ccgacgagcg gccctacacc
     3661 tgtgacatct gccacaaagc cttccgaagg caagaccacc tgcgggacca caggtgcggc
     3721 aatgcgggtg gcccaagaga gagctctctg cctctggcct caccatcttt ctccctaacg
     3781 agattcttga gaccccctac tggctccaag cctctggggg ttcagaaaat tctttaaaat
     3841 caagaaggaa agcctaggag acccagggat aggttttgga gcatgggttc aggaaatgtg
     3901 ggaataaaca ggacccaaga ttaagtgagg tctccgtgag ctccagacaa gcacatagct
     3961 tcctccatcc tcagggctgt ccagtagaag gttttgtggt gaaggaaatg tgacatgaga
     4021 catatgtgac agttaagtat ttgaaatgta atagtgcacc tgaaaaactg gttttattta
     4081 attttaagta atttaataaa tatttaataa gcacacgtgc tagtgtgtgc ttagcaccat
     4141 cttggacagt gcagatcaag caaattgtct gactccggct cccttaactc atcttcagtg
     4201 ttgacccagg ccttataagg aggccaagct tgcccctccc gcccaccagg aaggcaggta
     4261 actgaatctg tgtaaggatc agactttgct aaagcatctc cttttcctcc ccactccatc
     4321 cccaatcaac attttgtctc atccacactt ggtacccgac aacgcccttt tcctttgaga
     4381 acaaagtgcc ccatagcagt gggaggggat cttgggcaat tgtttataat attgcatcat
     4441 tttgcaagga tgaactctgt ttctgccctc agatatattc actccaaaga gaagcccttc
     4501 aagtgtcaag agtgtgggaa aggattctgc cagtccagga ctctcgctgt ccacaagacg
     4561 ctacactcac aggtgaagga gctcaaaacc tccaagatca aatgcgctag cgccactaac
     4621 ttctccctgt tgaaacaagc aggggatgtc gaagagaatc ccgggccaat ggtgagcaag
     4681 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     4741 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     4801 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     4861 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     4921 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     4981 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     5041 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5101 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     5161 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     5221 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     5281 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     5341 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaga attccgatca
     5401 tattcaataa cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtctg
     5461 aagaggagtt tacgtccagc catctttttc cctctgccaa aaattatggg gacatcatga
     5521 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     5581 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     5641 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     5701 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     5761 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     5821 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     5881 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     5941 agaagcttag gatctcgacc tcgaaattct accgggtagg ggaggcgctt ttcccaaggc
     6001 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     6061 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     6121 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     6181 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     6241 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     6301 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     6361 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     6421 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     6481 tccatctaga tctcgagcag ctgaagctta ccatgaccga gtacaagccc acggtgcgcc
     6541 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     6601 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     6661 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6721 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6781 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6841 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     6901 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6961 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     7021 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     7081 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     7141 ccgaaaggag cgcacgaccc catgcatcga tgatatcaga tccccgggat gcagaaattg
     7201 atgatctatt aaacaataaa gatgtccact aaaatggaag tttttcctgt catactttgt
     7261 taagaagggt gagaacagag tacctacatt ttgaatggaa ggattggagc tacgggggtg
     7321 ggggtggggt gggattagat aaatgcctgc tctttactga aggctcttta ctattgcttt
     7381 atgataatgt ttcatagttg gatatcataa tttaaacaag caaaaccaaa ttaagggcca
     7441 gctcattcct cccactcatg atctatagat ctatagatct ctcgtgggat cattgttttt
     7501 ctcttgattc ccactttgtg gttctaagta ctgtggtttc caaatgtgtc agtttcatag
     7561 cctgaagaac gagatcagca gcctctgttc cacatacact tcattctcag tattgttttg
     7621 ccaagttcta attccatcag aagctggtcg agatccggaa cccttaatat aacttcgtat
     7681 aatgtatgct atacgaagtt attaggtccc tcgaagaggt tcactaggcg cgccatagag
     7741 cctccgggtt acaaggaccc tgggcacagc ggccctctcc tccagaggga ccagaagcct
     7801 aactctggcg ggcaggggag aggcgctcgg cttgttcagg accttccacc tctccaacat
     7861 tgtcccctgg gtccctggcg cacgcggcac ttcagagccc cgcccggggc ctagacccag
     7921 accgacccgg ctgctcccct aggacgcggc taaactcttg gccaaaaggc ggtactcacg
     7981 tggcggaagg gaaactgcat taaaaaaaga acgaacgctc cgtagagctg cagcggcgcc
     8041 tctcccagaa gtattacttt ttctattgtt attttatacg ttttcatttt tatttttgtc
     8101 tttgaccata taagcttgta actctgactg cggagagtga ggggagaagg gagaagcaac
     8161 gaagtccttc gcactggcac ctcccagctc ctcctcctgc ccctgccccc accccggagg
     8221 ctgcaaaagt gtaatcccgg tatctacttc agccgtctgc cccgggaccg cggggccagg
     8281 agcgccctct cccagctctt gcagctcaac cgggctccga gactcagaca gccgtgcgag
     8341 gacctccggc ctgggggctg caggggaccc ggcctctgcc gcctagacac cacgagggag
     8401 cagccagcca ggagccgggc gttatattac gattggcact ttatgctgac catcggtaac
     8461 ggacatttat cactggagtt tttttttttt tttaactatt aaataaacgg ttattttaca
     8521 ggcattgcag gatgggtaat ttccctccta aacgtgggaa accgggtggg tcttctcttt
     8581 gagatgacca ggtttctttg caggacgcca gtgtgtcccg ctccttccac cctgtcttat
     8641 taggacgaca gggctgaaat ggatagagaa atgccccctc tcccgggtag gtctgcctct
     8701 gccccaccat gtattttctt acttttttcc tccacctcca gggtagggtc tcagctgtcc
     8761 cactcctcac ctctaaaggg ctgtgagttt aggaagaacc taagcctgaa agcagaacac
     8821 tggccgtgag aaatcccagc gtctctgggt gctgaggtcg cttagggtgc aagaagagaa
     8881 gagaatgtcg ttccccaccc taaccgaaga cttccctcct ggcctctggc tttggggtgg
     8941 gaaagaacca ccctcacaga gcctcagcgc tgtattgttt cctgttccca ggggtcccaa
     9001 cactgggctg cagatacgca atcactgctg cctccccagg agctcctggt cgagcagtgt
     9061 ggttttcaag aggaagcaaa aagcctctcc acccaggcct ggaatgtttc cacccaatgt
     9121 cgagcagtgt ggttttgcaa gaggaagcaa aaagcctctc cacccaggcc tggaatgttt
     9181 ccacccaatg tcgagcaaac cccgcccagc gtcttgtcat tggcgaattc gaacacgcag
     9241 atgcagtcgg ggcggcgcgg tcccaggtcc acttcgcata ttaaggtgac gcgtgtggcc
     9301 tcgaacaccg agcgaccctg caggtcctcg ccatggatcc tgatgatgtt gttgattctt
     9361 ctaaatcttt tgtgatggaa aacttttctt cgtaccacgg gactaaacct ggttatgtag
     9421 attccattca aaaaggtata caaaagccaa aatctggtac acaaggaaat tatgacgatg
     9481 attggaaagg gttttatagt accgacaata aatacgacgc tgcgggatac tctgtagata
     9541 atgaaaaccc gctctctgga aaagctggag gcgtggtcaa agtgacgtat ccaggactga
     9601 cgaaggttct cgcactaaaa gtggataatg ccgaaactat taagaaagag ttaggtttaa
     9661 gtctcactga accgttgatg gagcaagtcg gaacggaaga gtttatcaaa aggttcggtg
     9721 atggtgcttc gcgtgtagtg ctcagccttc ccttcgctga ggggagttct agcgttgaat
     9781 atattaataa ctgggaacag gcgaaagcgt taagcgtaga acttgagatt aattttgaaa
     9841 cccgtggaaa acgtggccaa gatgcgatgt atgagtatat ggctcaagcc tgtgcaggaa
     9901 atcgtgtcag gcgatctctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     9961 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
    10021 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
    10081 tgggagcagt ggtggaatgc agatccacta gagtcgaagc ggcttcactc ctcaggtgca
    10141 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
    10201 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
    10261 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
    10321 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
    10381 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
    10441 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
    10501 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
    10561 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
    10621 catagctgtc cctcttctct tatggagatc agctcggtac caagcttggc gtaatcatgg
    10681 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    10741 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    10801 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    10861 ggccaacgcg cggggagagg cggtttgcgt attgggcgct
//
