LOCUS       sequence-37460-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Valastyan JS, Lindquist S
  TITLE     TorsinA and the TorsinA-Interacting Protein Printor Have No Impact 
            on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast.
  JOURNAL   PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26.
  PUBMED    21818381
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22744. Epub 2011 Jul 26"
COMMENT     Sequence Label: 413-Met25 Kar2 TorsinA E171Q HDEL
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        61..410
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     411..427
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     411..427
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    435..459
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acctcctaag ggacaaaagc tggagctccg gatgcaaggg ttcgaatccc ttagctctca
       61 ttattttttg ctttttctct tgaggtcaca tgatcgcaaa atggcaaatg gcacgtgaag
      121 ctgtcgatat tggggaactg tggtggttgg caaatgacta attaagttag tcaaggcgcc
      181 atcctcatga aaactgtgta acataataac cgaagtgtcg aaaaggtggc accttgtcca
      241 attgaacacg ctcgatgaaa aaaataagat atatataagg ttaagtaaag cgtctgttag
      301 aaaggaagtt tttccttttt cttgctctct tgtcttttca tctactattt ccttcgtgta
      361 atacagggtc gtcagataca tagatacaat tctattaccc ccatccatac tctagaacta
      421 gtggatcccc catcacaagt ttgtacaaaa aagcaggctt cacaaaatgt ttttcaacag
      481 actaagcgct ggcaagctgc tggtaccact ctccgtggtc ctgtacgccc ttttcgtggt
      541 aatattacct ttacagaatt ctttccactc ctccaatgtt ttagttagag gtgtggagcc
      601 catcagcctg ggactggccc tggccggcgt cctcaccggc tacatctacc cgcgtctcta
      661 ctgcctcttc gccgagtgct gcgggcagaa gcggagcctt agccgggagg cactgcagaa
      721 ggatctggac gacaacctct ttggacagca tcttgcaaag aaaatcatct taaatgccgt
      781 gtttggtttc ataaacaacc caaagcccaa gaaacctctc acgctctccc tgcacgggtg
      841 gacaggcacc gg
//
