LOCUS       sequence-374623-       12489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12489)
  AUTHORS   Brehm JN, Sorg JA
  TITLE     Plasmid Sequence and Availability for an Improved Clostridioides 
            difficile CRISPR-Cas9 Mutagenesis System.
  JOURNAL   Microbiol Resour Announc. 2022 Dec 15;11(12):e0083322. doi: 
            10.1128/mra.00833-22. Epub 2022 Nov 7.
  PUBMED    36342279
REFERENCE   2  (bases 1 to 12489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Resour Announc."; date: "2022-12-15"; volume: "11(12):e0083322. 
            doi"; pages: " 10.1128/mra.00833-22. Epub 2022 Nov 7"
COMMENT     Sequence Label: pJB06
FEATURES             Location/Qualifiers
     source          1..12489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1480..5586
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1480..5583
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(5616..5633)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5616..5632)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5625..5647)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10158..10746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10647..10666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10900..10917
                     /label=L4440
                     /note="L4440 vector, forward primer"
     oriT            11842..11951
                     /note="incP origin of transfer"
     CDS             11984..12355
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccgccc cttatatttc attaattaaa gttaaatttt
      121 gtatattcaa aaagctcttg atatttacgg cagagccacc atatagagat gcctcagacc
      181 ctaatgatga aatttctata tttattcctt tgttaaaaga gctaactaac atatcttttg
      241 taatctgtaa tatctcaggg atatcactaa ttatttggct atttaaatat attatttcag
      301 gagcataagt tgtaatagca ttatttatag ctattgttaa gtaactacaa aactcatgaa
      361 taactttttt ggcattctgg ttatcttcat aatatagttg ctttactata tcagaatcta
      421 ttttaggaat attttctaaa gaagaaagct gttcaaatac ctttttttct gaacaatact
      481 gttctaaaca gccacgattt ccacaaggac atagtttacc attaggcatg atgatagtgt
      541 gaccaatttc tccactcata ccatttctgc cgctatataa tttattattt attataatgc
      601 ctgaaccaaa tccactgtga atactgagac taagtagtga gttatgtaca gttgaaaaag
      661 tattctcagc taaagctgtt aagtttgctt cattttctat atgaatagga aagtcatact
      721 ttttgctaag gatgctatat aagtcaatct cattaagatt ataatatgga gtaaacaata
      781 ctttattttc acaagtaatc ccatgtatag ccaaagtaag accaattacc ttataaggag
      841 tgtctatttt ggatatgtta taactattta taatttcatc tatcaattgt ataacattgt
      901 ctttacttac ttgtatatct gttaatttct tagaatttat aatagttcca tctaagtaag
      961 atagagaaga aaatatatag tcgtatccaa tgtccatact taaagaaatc cctgcacatt
     1021 tattaaatac taataatatg ggttttcttc cgccactatg agtactattt ccaattccta
     1081 tttcatgaac tagagattca tcaataagtt ttttggtaat agcggatata gttgctttat
     1141 tcaatcctat agtagaagct atacttgccc tagaaatagg accatttttt ataatttgtt
     1201 caagtaccaa tctttcattc atttctcgaa tagtgtattt atcagtaacc aatttgatat
     1261 tcctccttaa aataatattg taatactttt tacacaaaaa taaaaggtta ttttgcattg
     1321 acaaagataa ttaaatattt tattattagt tcataagtta gtttaatata ctaacaaaaa
     1381 taaagcaagt aaaatatacc taaaatataa aaaaattagg ataggaaaac gatagttatg
     1441 aagtggcatt caaggagggt aaagaggaga gttaattaaa tggataaaaa atatagtata
     1501 ggattagata taggaacaaa tagtgtagga tgggcagtaa taactgatga atataaagta
     1561 ccaagtaaaa aatttaaagt attaggaaat acagatagac atagtataaa aaaaaattta
     1621 ataggtgcat tactttttga tagtggtgaa acagcagaag caacaagact taaaagaaca
     1681 gcaagaagaa gatatactag aagaaaaaat agaatatgtt atttacaaga aatatttagt
     1741 aatgaaatgg caaaagtaga tgatagtttt tttcatagat tagaagaaag ttttttagta
     1801 gaagaagata aaaaacacga aagacatcca atatttggaa atatagtaga tgaagtagca
     1861 tatcatgaaa aatatccaac aatatatcat ttaagaaaaa aacttgtaga tagtacagat
     1921 aaagcagatt taagacttat atatcttgca ttagcacata tgataaaatt tagaggacat
     1981 tttcttatag aaggtgattt aaatccagat aatagtgatg tagataaact ttttatacaa
     2041 ttagtacaaa catataatca attatttgaa gaaaatccaa taaatgcaag tggtgtagat
     2101 gcaaaagcaa tattaagtgc aagattaagt aaaagtagaa gattagaaaa tcttatagca
     2161 caattacctg gagaaaaaaa aaatggatta tttggtaatc ttatagcttt aagtttagga
     2221 ttaacaccaa attttaaatc aaattttgat cttgcagaag atgcaaaact tcaattaagt
     2281 aaagatacat atgatgatga tttagataat ttacttgcac aaataggtga tcaatatgca
     2341 gatttatttc ttgcagcaaa aaatctttct gatgcaatac ttttaagtga tatacttaga
     2401 gtaaatacag aaataacaaa agcaccatta tctgcaagta tgataaaaag atatgatgaa
     2461 catcatcaag atttaacatt acttaaagca ttagtaagac aacaattacc agaaaaatat
     2521 aaagaaattt tttttgatca aagtaaaaat ggatatgcag gatatataga tggtggtgca
     2581 agtcaagaag aattttataa atttattaaa ccaatattag aaaaaatgga tggaacagaa
     2641 gaattattag taaaacttaa tagagaagat ttacttagaa aacaaagaac atttgataat
     2701 ggaagtatac cacatcaaat acatttaggt gaattacatg caatattaag aagacaagaa
     2761 gatttttatc catttcttaa agataataga gaaaaaattg aaaaaatact tacatttaga
     2821 ataccatatt atgtaggacc attagcaaga ggaaatagta gatttgcttg gatgacaaga
     2881 aaaagtgaag aaacaataac accatggaat tttgaagaag tagtagataa aggagcatct
     2941 gcacaaagtt ttatagaaag aatgacaaat tttgataaaa atcttccaaa tgaaaaagta
     3001 ttaccaaaac atagtttatt atatgaatat tttacagtat ataatgaatt aacaaaagta
     3061 aaatatgtaa cagaaggtat gagaaaacca gcatttttaa gtggtgaaca aaaaaaagct
     3121 atagtagatt tattatttaa aacaaataga aaagtaacag taaaacaatt aaaagaagat
     3181 tattttaaaa aaattgaatg ttttgatagt gtagaaatat ctggtgtaga agatagattt
     3241 aatgcaagtt taggaacata tcatgattta cttaaaataa taaaggataa agatttttta
     3301 gataatgaag aaaatgaaga tattttagaa gatatagtat taacattaac tttatttgaa
     3361 gatagagaaa tgatagaaga aagattaaaa acttatgctc atttatttga tgataaagta
     3421 atgaaacaac ttaaaagaag aagatataca ggttggggaa gattaagtag aaaacttata
     3481 aatggaataa gagataaaca aagtggaaaa acaatattag attttcttaa aagtgatgga
     3541 tttgcaaata gaaattttat gcaattaata catgatgata gtttaacatt taaagaagat
     3601 attcaaaaag cacaagtaag tggacaaggt gatagtttac atgaacatat agcaaattta
     3661 gcaggatctc cagcaataaa aaaaggtata ttacaaacag taaaagtagt tgatgaatta
     3721 gtaaaagtaa tgggtagaca taaaccagaa aatatagtta tagaaatggc aagagaaaat
     3781 caaacaacac aaaaaggaca aaaaaatagt agagaaagaa tgaaaagaat agaagaaggt
     3841 ataaaagaat taggaagtca aatacttaaa gaacatccag tagaaaatac acaacttcaa
     3901 aatgaaaaac tttatttata ttatttacaa aatggaagag atatgtatgt agatcaagaa
     3961 ttagatataa atagattaag tgattatgat gtagatcata tagtaccaca aagttttctt
     4021 aaagatgatt ctatagataa taaagtatta acaagatctg ataaaaatag aggtaaaagt
     4081 gataatgtac caagtgaaga agttgtaaaa aaaatgaaaa attattggag acaattactt
     4141 aatgcaaaac ttataacaca aagaaaattt gataatctta caaaagcaga aagaggtgga
     4201 ttatctgaat tagataaagc tggatttata aaaagacaat tagtagaaac tagacaaata
     4261 acaaaacatg tagcacaaat attagatagt agaatgaata caaaatatga tgaaaatgat
     4321 aagcttataa gagaagtaaa agttataaca ttaaaaagta aattagtaag tgattttaga
     4381 aaagattttc aattttataa agtaagagaa ataaataatt atcatcatgc acatgatgca
     4441 tatcttaatg cagtagtagg aacagcatta ataaaaaaat atcctaaatt agaatctgaa
     4501 tttgtatatg gagattataa agtatatgat gtaagaaaaa tgatagcaaa aagtgaacaa
     4561 gaaataggaa aagcaacagc aaagtacttt ttttattcta atataatgaa tttttttaag
     4621 acagaaataa ctttagcaaa tggtgaaata agaaaaagac ctttaataga aactaatggt
     4681 gaaacaggtg aaatagtatg ggataaaggt agagattttg caacagtaag aaaagtatta
     4741 agtatgccac aagtaaatat agtaaaaaaa acagaagtac aaactggagg atttagtaaa
     4801 gaaagtatac ttccaaaaag aaattcagat aaacttattg caagaaaaaa agattgggat
     4861 ccaaaaaaat atggtggatt tgatagtcca acagtagcat atagtgtatt agtagtagca
     4921 aaagtagaaa aaggtaaatc taaaaaactt aaaagtgtaa aagaattact tggaataaca
     4981 ataatggaaa gaagtagttt tgaaaaaaat cctattgatt ttttagaagc aaaaggatat
     5041 aaagaagtta aaaaagattt aataataaaa cttccaaaat attctttatt tgaattagaa
     5101 aatggaagaa aaagaatgtt agcaagtgct ggtgaattac aaaaaggtaa tgaattagca
     5161 ttaccatcta aatatgtaaa ttttctttat ttagcaagtc attatgaaaa acttaaagga
     5221 tctcctgaag ataatgaaca aaaacaatta tttgtagaac aacataaaca ttatttagat
     5281 gaaataattg aacaaataag cgaattttct aaaagagtaa tattagcaga cgcaaattta
     5341 gataaagtat tatctgctta taataaacat agagataaac caataagaga acaagcagaa
     5401 aatataatac atctttttac attaacaaat ttaggtgcac cagcagcatt taaatatttt
     5461 gatacaacaa tagatagaaa aagatataca agtacaaaag aagtattaga cgcaacatta
     5521 atacatcaaa gtataacagg attatatgaa acaagaatag atttatctca attaggtggt
     5581 gattaactcg aggcctgcag acatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt
     5641 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     5701 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     5761 aatggcgaat ggcgctagca taaaaataag aagcctgcat ttgcaggctt cttattttta
     5821 tggcgcgccc gcccttaagt ctaaaaatta ggggagatgt aaggatttgg gaaaaataga
     5881 agatgttata atcataaata tggtattcgt aggcttaaag tcaaaaagga ggtgaaatat
     5941 aaatagattt ttagctaaat taagtaagaa ataggaggag atttattgaa caaaaaatta
     6001 gaaaaaccat ttgtatataa gagagagtac gatttgactg gatatgatgt tgaaatttta
     6061 caaaaatatg agttagaaca agcaatatat gtttatgttg ggagtagttg tgcatataac
     6121 atgagagcta gaagtagtaa atggagatac catataagaa caaataataa gtctatatgt
     6181 tgtaacatta aaaattttat acataacttg gaattgtttt ataaaatgga attaaagttg
     6241 tcagataata ttattaatga taagctatac tatagcaata tagcagagtt tgaagaattt
     6301 gaaacactag aaaaagctag agaggtagaa agtactatta taagtcaata tcaattttta
     6361 gattctataa atcacatgtt aaaacaaaaa ataattttat tgagtaataa ggatagtgtg
     6421 ttaaacataa ctaaaaatgg aaatacaaat tatttgaaag taaaaaataa atacatagaa
     6481 aaacataaga acaagccaat aatgagatac catatcaact gtcaattcaa tacagatgga
     6541 agtgtcaaaa gtattacaca ggagtttgaa ccaatattgg aattaaacaa aaaaaatacc
     6601 ctaagccgac caagcagagt atttttaaaa taatatttta agataacaac aaaatgagat
     6661 aatactacta gacaatgaca actcaactac caattgagtt tatggagcta ccaactccaa
     6721 tatcggtcta actgattaag tatctgtagt tatataataa tattgctatc aattttagca
     6781 tcttaacaat attattatac atactaagct aaaattattc aatagttgta aaagttgatt
     6841 agtcaataag tatatattta atgtagtgtt atctcttaaa aaaactagat aaggagataa
     6901 taaatatatg gaacaattag attcaaaata taagttgaaa aaatttctaa tggcagtatt
     6961 tagagatggt ataggacaag gaaataatct tattgataat gaatatgtta gagtatttca
     7021 aaataataaa agtaatagta aacaattaga actcggagaa gaatttaaag aatatagtaa
     7081 aacaactttt tttaaaaata tagatgatat agtagaattt accttcgcaa aaaatattta
     7141 ttatgaaaat acatttttta acctatgtac tactgatgga aaagcaggaa ccaatgaaaa
     7201 cttaataaat agatatgcat taggatttga ttttgacaaa aaagaattag gacaaggttt
     7261 taattataaa gatataatta atttatttac taagatagga ttacattatc atatcctagt
     7321 tgatagtgga aatggattcc atgtttatgt gctaattaat aaaactaata acattaagtt
     7381 agtatcagaa gttacaaata cattaataaa taaattgggt gcagataaac aagcaaattt
     7441 atctactcaa gtattaagag taccttatac atataatatt aaaaatacta ctaaacaagt
     7501 aaaaataata caccaagaca aaaatatata tagatatgac atagaaaagt tagctaaaaa
     7561 atattgcaaa gatgtaaaaa cagtaggtaa tactaataca aaatatatat tagatagtaa
     7621 gctaccaaat tgtatagtag atattttaaa aaatggtagt aaagatggac ataaaaacct
     7681 agatttgcaa aaaatagttg tgactttaag attgaggaat aaaagtttaa gtcaagtaat
     7741 atccgttgct agagaatgga actatatatc acaaaatagt ctttcaaata gtgagctaga
     7801 atatcaagtc aagtatatgt atgagaaact taaaacggtt aattttggtt gtactggttg
     7861 tgagtttaat agtgattgtt ggaataaaat agaatcagat tttatatata gtgatgaaga
     7921 tactttgttc aatatgccac ataagcactc aaaggatttg aaatataaga ataggaaagg
     7981 ggttaaaata atgactggta atcaattgtt tatctataat gtgttactta acaataaaga
     8041 tagagaatta aacatagacg atataatgga gctgataacc tataaacgta agaagaaagt
     8101 taaaaacatt gttatgagtg aaaagacatt aagagaaaca ttaaaagaac ttcaacataa
     8161 tgattatatt acaaaaacaa aaggtgttac aaagctagga ataaaagata catacaatgt
     8221 aaaagaagtt agatgtaata tagataaaca atatactatt agttactttg ttaccatggc
     8281 agtaatttgg ggaataattt caactgaaga attaagatta tatactcaca tgagatataa
     8341 gcaagattta ttggtcaaag atgataaaat aaaaggaaat atattaagaa ttaatcaaga
     8401 ggaattagca aaagatttag gagtaacaca gcaaagaatt tcaaatatga tagaatcttt
     8461 attagatact aaaattttag atgtatggga aactaaaata aatgatagag gatttatgta
     8521 ctatacatat agattaaaca agtagatttt tgataggatt agaattgatt ttctagtcct
     8581 atttttatgc aaaaaaacta attaataaaa atttcttttg gtaaaataat tgtacgagaa
     8641 ttgcaaaaaa aaaatggcat caaagtattg aaattaagcc gttttaaaaa tttcttttgg
     8701 taaaataatt ctacatatat atgtagtata tatatatatg ttttttagag aatgtataac
     8761 tagaatatag agctagaata tagagaatgt ataactagaa tatagagcta gaatatagag
     8821 aatgtataac tagaatatag agctagaata tagagaatgt ataactagaa tatagagcta
     8881 gaatatagag aatgtataac tagaatatag agctagaata tagagaatgt ataactagaa
     8941 tatagagcta gaatatagag aatgtataac tagaatatag agctagaatc ctaattagta
     9001 ggtgcttttt taaaacaagt taaaaatcaa aaatagtatt agtaagcatt ggaaatgcta
     9061 gattctaaaa tagaaaagta aaaaattggt gcactatcta aacttatcta tatcgctttt
     9121 tccgtcgttt ggttctctag ttacgataca ggggatatgc ttatattgag ttatagtact
     9181 aatcagtgct taatatagtt aataaaatta tagttaccat agtttagtaa ctatgatgta
     9241 tgttagttag aaacttgcat ttcggccggc cagtgggcaa gttgaaaaat tcacaaaaat
     9301 gtggtataat atctttgttc attagagcga taaacttgaa tttgagaggg aacttagatg
     9361 gtatttgaaa aaattgataa aaatagttgg aacagaaaag agtattttga ccactacttt
     9421 gcaagtgtac cttgtaccta cagcatgacc gttaaagtgg atatcacaca aataaaggaa
     9481 aagggaatga aactatatcc tgcaatgctt tattatattg caatgattgt aaaccgccat
     9541 tcagagttta ggacggcaat caatcaagat ggtgaattgg ggatatatga tgagatgata
     9601 ccaagctata caatatttca caatgatact gaaacatttt ccagcctttg gactgagtgt
     9661 aagtctgact ttaaatcatt tttagcagat tatgaaagtg atacgcaacg gtatggaaac
     9721 aatcatagaa tggaaggaaa gccaaatgct ccggaaaaca tttttaatgt atctatgata
     9781 ccgtggtcaa ccttcgatgg ctttaatctg aatttgcaga aaggatatga ttatttgatt
     9841 cctattttta ctatggggaa atattataaa gaagataaca aaattatact tcctttggca
     9901 attcaagttc atcacgcagt atgtgacgga tttcacattt gccgttttgt aaacgaattg
     9961 caggaattga taaatagtta acttcaggtt tgtctgtaac taaaaacaag tatttaagca
    10021 aaaacatcgt agaaatacgg tgttttttgt taccctaagt ttaaactcct ttttgataat
    10081 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10141 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10201 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10261 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    10321 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10381 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    10441 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10501 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    10561 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    10621 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    10681 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    10741 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    10801 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    10861 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    10921 gcggaagagc gcccaatacg cagggcccta acatcttcta tttttcccaa atccttacat
    10981 ctcccctaat ttttagactt aagggcgaat tccaaaggac gaatatgtcg cctaaagcat
    11041 aatgctctga ataagaatag aaaccatagt cctcatgaag aaaataggac agtttcagtt
    11101 gtaagatgtt ttcgaccacc tgctgtacgt ctgttgtcgg aaagcgaatc cttacataat
    11161 caaacagcat ttcaagggga gcgtcgggat tgaagcgttc cagagcttcc caaagggact
    11221 gctgtaaatc ctctgatggc ttgacttttc ctgtttcaat atcgcttaga tactgccttg
    11281 taataccagt cgcaacagct aaacggtttt gagatagtcc ataagccaag cgtttttctt
    11341 ttaaatgctg taaccaagtt tgttcattca gtaaaaatcc ctccaatcaa aaaggcgtat
    11401 gtcaactttt aaagcccatt tgacatacgc tgaaattttg taaatccctt gtaaccaaag
    11461 gattttctaa tgtttttttg actgtttcct gtcgatttgt acccccctgt tagatacggg
    11521 gggttaagtg ctggcgtggc tattgccaca ccagccagca agatcagtcc acacctgcga
    11581 cttccgcttc gcacgtcgcc tgcgtggact gtctgctgtt ggataacttt ttaatttcct
    11641 ccaagaaatc atatcctttt ggtacaaggg gagtataaaa gaattctgca gattacctaa
    11701 taatttatct acattccctt tagcctgctt cggggtcatt atagcgattt tttcggtata
    11761 tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg
    11821 gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg
    11881 ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg
    11941 aggctggccg gctaccgccg gcgtaacaga tgagggcaag cggatggctg atgaaaccaa
    12001 gccaaccagg aagggcagcc cacctatcaa ggtgtactgc cttccagacg aacgaagagc
    12061 gattgaggaa aaggcggcgg cggccggcat gagcctgtcg gcctacctgc tggccgtcgg
    12121 ccagggctac aaaatcacgg gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat
    12181 caatggcgac ctgggccgcc tgggcggcct gctgaaactc tggctcaccg acgacccgcg
    12241 cacggcgcgg ttcggtgatg ccacgatcct cgccctgctg gcgaagatcg aagagaagca
    12301 ggacgagctt ggcaaggtca tgatgggcgt ggtccgcccg agggcagagc catgactttt
    12361 ttagccgcta aaacggccgg ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca
    12421 tcaagaagag cgacttcgcg gagctggtga agtacatcac cgacgagcaa ggcaagaccg
    12481 atcgggccc
//
