LOCUS       sequence-37463-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Valastyan JS, Lindquist S
  TITLE     TorsinA and the TorsinA-Interacting Protein Printor Have No Impact 
            on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast.
  JOURNAL   PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26.
  PUBMED    21818381
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22744. Epub 2011 Jul 26"
COMMENT     Sequence Label: 413-Met25 Kar2 TorsinADE E171Q HDEL
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        66..415
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     416..432
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     416..432
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    440..464
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cattaccctc ctaagggaca aaagctggag ctccggatgc aagggttcga atcccttagc
       61 tctcattatt ttttgctttt tctcttgagg tcacatgatc gcaaaatggc aaatggcacg
      121 tgaagctgtc gatattgggg aactgtggtg gttggcaaat gactaattaa gttagtcaag
      181 gcgccatcct catgaaaact gtgtaacata ataaccgaag tgtcgaaaag gtggcacctt
      241 gtccaattga acacgctcga tgaaaaaaat aagatatata taaggttaag taaagcgtct
      301 gttagaaagg aagtttttcc tttttcttgc tctcttgtct tttcatctac tatttccttc
      361 gtgtaataca gggtcgtcag atacatagat acaattctat tacccccatc catactctag
      421 aactagtgga tcccccatca caagtttgta caaaaaagca ggcttcacaa aatgtttttc
      481 aacagactaa gcgctggcaa gctgctggta ccactctccg tggtcctgta cgcccttttc
      541 gtggtaatat tacctttaca gaattctttc cactcctcca atgttttagt tagaggtgtg
      601 gagcccatca gcctgggact ggccctggcc ggcgtcctca ccggctacat ctacccgcgt
      661 ctctactgcc tcttcgccga gtgctgcggg cagaagcgga gccttagccg ggaggcactg
      721 cagaaggatc tggacgacaa cctctttgga cagcatcttg caaagaaaat catcttaaat
      781 gccgtgtttg gtttcataaa caacccaaag cccaagaaac ctctcacgct ctccctgcac
      841 gggtggacag gcaccggcaa aatttcgtca gcagaatcat cgcag
//
