LOCUS       sequence-37466-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Valastyan JS, Lindquist S
  TITLE     TorsinA and the TorsinA-Interacting Protein Printor Have No Impact 
            on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast.
  JOURNAL   PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26.
  PUBMED    21818381
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22744. Epub 2011 Jul 26"
COMMENT     Sequence Label: 413-Met25 Kar2 TorsinA K108A GFP
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..412
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     413..429
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     413..429
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    437..461
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attacctcct aagggacaaa gctggagctc cggatgcaag ggttcgaatc ccttagctct
       61 cattattttt tgctttttct cttgaggtca catgatcgca aaatggcaaa tggcacgtga
      121 agctgtcgat attggggaac tgtggtggtt ggcaaatgac taattaagtt agtcaaggcg
      181 ccatcctcat gaaaactgtg taacataata accgaagtgt cgaaaaggtg gcaccttgtc
      241 caattgaaca cgctcgatga aaaaaataag atatatataa ggttaagtaa agcgtctgtt
      301 agaaaggaag tttttccttt ttcttgctct cttgtctttt catctactat ttccttcgtg
      361 taatacaggg tcgtcagata catagataca attctattac ccccatccat actctagaac
      421 tagtggatcc cccatcacaa gtttgtacaa aaaagcaggc ttcacaaaat gtttttcaac
      481 agactaagcg ctggcaagct gctggtacca ctctccgtgg tcctgtacgc ccttttcgtg
      541 gtaatattac ctttacagaa ttctttccac tcctccaatg ttttagttag aggtgtggag
      601 cccatcagcc tgggactggc cctggccggc gtcctcaccg gctacatcta cccgcgtctc
      661 tactgcctct tcgccgagtg ctgcgggcag aagcggagcc ttagccggga ggcactgcag
      721 aaggatctgg acgacaacct ctttggacag catcttgcaa agaaaatcat cttaaatgcc
      781 gtgtttggtt tcataaacaa cccaaagccc aagaaacctc tcacgctctc cctgcacggg
      841 tggacaggca ccggcgctaa tttcgtcagc aagatcatcg cagag
//
