LOCUS       sequence-374681-        3865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  400 nt l31 promoter region, l31 5'UTR with nt. 47-54.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Rasmussen RA, Wang S, Camarillo JM, Sosnowski V, Cho BK, Goo YA, 
            Lucks JB, O'Halloran TV
  TITLE     Zur and zinc increase expression of E. coli ribosomal protein L31 
            through RNA-mediated repression of the repressor L31p.
  JOURNAL   Nucleic Acids Res. 2022 Dec 9;50(22):12739-12753. doi: 
            10.1093/nar/gkac1086.
  PUBMED    36533433
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-12-9- 9"; volume: "50"; issue: 
            "22"; pages: "12739-12753"
COMMENT     Sequence Label: pRAR041
FEATURES             Location/Qualifiers
     source          1..3865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(623..625,626..1339)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             623..1336
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(653..681)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1276..1296
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1359..1388
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1404..1421
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1477..1494)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1647..1693
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1846..2505
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVNPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1912..1931)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2526..2620
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(3027..3044)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3161..3706)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3241..3260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acactttatg cttccggctg aattctaaag atctcggcaa ggcatgaaac agcacatcgc
       61 caatcggatg atgatagtaa tctgccgccc atagcagcaa tcgccagacg gagtgagtaa
      121 acaccggctc actatccagc acttcgacta ccgcttttag ctcattgagc ggcagttcgc
      181 tggcatcgct aactgatacc acaatcccga tgcgctcctg ctgtttgcca aacggcacgc
      241 gcacgcgaca cccagcttta accgtcatgc cttctggcag cagatagtca aaggtacgag
      301 gaagcggaac gggcaaggca acgtgggcaa cgggcatagc atcatcctga cttgaaattc
      361 ggtgggttag tatacacatt gccgtagaag agtgcggatc agtttgcata cgctggttaa
      421 tttctgtatg atttcgcgcc ttcgtacgaa atgatcgtat tgaagctata cttttaacat
      481 gtctggcgtt agggctggaa gcttaaaccg aggttttccc atgaaaaaag atattcaccc
      541 gaaatacgaa gaaattactg ctagctgctc ttgcggtaac gtaatgaaaa tccgctccac
      601 cgttggtcat ggtggtggtg gtatgagcaa aggagaagaa cttttcactg gagttgtccc
      661 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtcc gtggagaggg
      721 tgaaggtgat gctacaaacg gaaaactcac ccttaaattt atttgcacta ctggaaaact
      781 acctgttccg tggccaacac ttgtcactac tctgacctat ggtgttcaat gcttttcccg
      841 ttatccggat cacatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt
      901 acaggaacgc actatatctt tcaaagatga cgggacctac aagacgcgtg ctgaagtcaa
      961 gtttgaaggt gatacccttg ttaatcgtat cgagttaaag ggtattgatt ttaaagaaga
     1021 tggaaacatt cttggacaca aactcgagta caactttaac tcacacaatg tatacatcac
     1081 ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa attcgccaca acgttgaaga
     1141 tggttccgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
     1201 ccttttacca gacaaccatt acctgtcgac acaatctgtc ctttcgaaag atcccaacga
     1261 aaagcgtgac cacatggtcc ttcttgagtt tgtaactgct gctgggatta cacatggcat
     1321 ggatgagctc tacaaataag gatctgaagc ttgggcccga acaaaaactc atctcagaag
     1381 aggatctgaa tagcgccgtc gaccatcatc atcatcatca ttgagtttaa acggtctcca
     1441 gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg
     1501 cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga
     1561 ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca
     1621 tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1681 cctttcgttt tatctgttgt ttgtcggtga actggatcct tactcgagtc tagactgcag
     1741 ttgatcgggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     1801 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     1861 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     1921 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     1981 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     2041 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg
     2101 gatagtgtta acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     2161 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     2221 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     2281 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     2341 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     2401 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     2461 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaatttga tatcgagctc
     2521 gcttggactc ctgttgatag atccagtaat gacctaagaa ctccatctgg atttgttcag
     2581 aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaagcctcc gatcaacgtc
     2641 tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac caggatttat
     2701 ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga
     2761 tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg
     2821 tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca
     2881 ccgccggaca tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg
     2941 tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat
     3001 atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
     3061 ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag
     3121 atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc
     3181 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat
     3241 aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc
     3301 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
     3361 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
     3421 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa
     3481 gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc
     3541 cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc
     3601 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt
     3661 ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat
     3721 taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg tagcacctga
     3781 agtcagcccc atacgatata agttgtaatt ctcatgtttg acagcttatc atcgataagc
     3841 ttccgatggc gcgccgagag gcttt
//
